FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11545934
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG10683299.252859058435636No Hit
CAACGGAATCCCAAAAGCAGCTG9766488.45880463200292No Hit
TTCAAGTAATCCAGGATAGGCT4039183.498357083974324No Hit
AACATTCAACGCTGTCGGTGAGT3993433.458732745224423No Hit
AAGCTGCCAGTTGAAGAACTGT3956353.42661754345729No Hit
TAGCAGCACGTAAATATTGGCG3551203.0757147927573465No Hit
CCCCCACTGCTAAATTTGACTG2687992.3280836353299783No Hit
AACATTCAACGCTGTCGGTGAG2147691.8601266904868847No Hit
GCCTGTCTGAGCGTCGCT1933051.6742257490818848No Hit
TCCACCACGTTCCCGTGG1836451.590559932180454No Hit
TAGCTTATCAGACTGATGTTGAC1519731.3162469142816857No Hit
TATTGCACTTGTCCCGGCCTGTAA1505561.3039741955912791No Hit
TATTGCACTTGTCCCGGCCTGTAT1348311.1677790640410728No Hit
TGAGGTAGTAGGTTGTATAGTT1309081.1338017348791358No Hit
TAGCTTATCAGACTGATGTTGACT1227981.0635605573355955No Hit
AGCAGCATTGTACAGGGCTATGA932610.807738897520114No Hit
TGTAAACATCCTTGACTGGAAGCT917480.7946347172952833No Hit
TATTGCACTTGTCCCGGCCTGT893260.7736576356663739No Hit
TAGCTTATCAGACTGATGTTGA781480.6768443332518617No Hit
CGCCTGTCTGAGCGTCGCT751630.6509910761658607No Hit
TGAGGTAGTAGATTGTATAGTT724530.6275196099336788No Hit
ACTGGACTTGGAGTCAGAAGGC663250.574444648652937No Hit
CAACGGAATCCCAAAAGCAGCTGT647560.5608554491996923No Hit
CCCATAAAGTAGAAAGCACTAC639850.5541777737513482No Hit
CCCCCACTGCTAAATTTGACTGGCTT625110.5414113747748774No Hit
TATTGCACTTGTCCCGGCCTGTA616580.5340234925992129No Hit
TAGCAGCACATCATGGTTTACA616290.5337723219273556No Hit
CATAAAGTAGAAAGCACTACT603750.5229113556339401No Hit
TGTAAACATCCCCGACTGGAAGCT566220.49040640627254584No Hit
TTCACAGTGGCTAAGTTCCGC523000.4529733151081584No Hit
CTACGCCTGTCTGAGCGTCGCT462730.4007731206500921No Hit
TTCAAGTAATTCAGGATAGGTT447780.3878248394629659No Hit
TCGTTTCCCGGCCAATGCACCA434860.37663475297884086No Hit
TTAATGCTAATCGTGATAGGGGTT427030.36985314483869386No Hit
TGTAAACATCCTACACTCTCAGCT397640.34439829640460445No Hit
TACGCCTGTCTGAGCGTCGCT394190.3414102315152676No Hit
TCAGGCTCAGTCCCCTCCCGAT383190.3318830680999909No Hit
CCCCCCACTGCTAAATTTGACTGGCT355500.3079005994664442No Hit
TGAGGTAGTAGTTTGTACAGTT341520.2957924408713925No Hit
CCCCCCACTGCTAAATTTGACTGG334070.289339952921955No Hit
AACATTCAACGCTGTCGGTGAGTT330460.2862133111102142No Hit
TCCTGTCTGAGCGTCGCT327130.28332917891268045No Hit
TTCTCACTACTGCACTTGACTAG320610.2776821693247164No Hit
CATTGCACTTGTCTCGGTCTGA316320.27396657559275844No Hit
CACCCGGCTGTGTGCACATGTGC314590.2724682126192649No Hit
TGTCAGTTTGTCAAATACCCCAA305880.2649244314058958No Hit
ATCACATTGCCAGGGATTTCC294050.25467840020564814No Hit
GTCAGGATGGCCGAGCGGTCTAAGG272090.23565871760569565No Hit
AACCGGGCGGAAACACCA259410.22467649650517665No Hit
TCGTACGACTCTTAGCGG258920.22425210468031428No Hit
CTTCTCACTACTGCACTTGACTAG254740.22063178258250912No Hit
ACGCCTGTCTGAGCGTCGCT233020.20181996536616265No Hit
AACGGAATCCCAAAAGCAGCTG228440.19785320096234743No Hit
TGAGGTAGTAGTTTGTGCTGTT227490.19703040048557352No Hit
CCCATAAAGTAGAAAGCACT226360.1960517009710951No Hit
TAGCAGCACGTAAATATTGGC225410.1952289004943212No Hit
TAGCTTATCAGACTGATGTTGACA225160.19501237405306493No Hit
CAAAGAATTCTCCTTTTGGGCT224260.19423287886454227No Hit
CTCCCACTGCTTCACTTGACTAG221870.19216288608613213No Hit
TGAGGTAGTAGGTTGTGTGGTT213550.18495688612112282No Hit
TAGCAGCACGTAAATATTGGCGT212700.18422069622085144No Hit
ATCACATTGCCAGGGATTTCCA207730.17991615056867638No Hit
TCAGGCTCAGTCCCCTCCCGA206860.1791626385531045No Hit
ACTGGACTTGGAGTCAGAAGGCA206370.17873824672824218No Hit
TGTCAGTTTGTCAAATACCCCA201300.17434709049956462No Hit
ACCCCCCACTGCTAAATTTGACTG192650.16685527563209698No Hit
CAACGGAATCCCAAAAGCAGCT188330.1631136987271883No Hit
AGCAGCATTGTACAGGGCTATGT186960.16192713382910381No Hit
TGTAAACATCCTACACTCAGCT184900.160142955953152No Hit
AGCTCGGTCTGAGGCCCCTCAGT179200.15520615309250857No Hit
TATTGCACTTGTCCCGGCCTGTT176330.15272042954688636No Hit
TATTGCACTTGTCCCGGCCTGTAG174290.15095357378623503No Hit
TCGTTTCCCGGCCAACGCACCA174220.15089294638268327No Hit
TTCAAGTAATCCAGGATAGGCTT170550.14771433822504096No Hit
TTCACAGTGGCTAAGTTCCG170240.14744584543788317No Hit
TTCAAGTAATCCAGGATAGGC168530.14596480457969013No Hit
GGGCTACGCCTGTCTGAGCGTCGCT164150.14217126132887994No Hit
TCGTTTCCCGGGCGGCGCACCA162410.14066423729773614No Hit
TGTAAACATCCCCGACTGGAAGC158260.13706989837288175No Hit
GCGTTCGCGCTTTCCCCTG156350.1354156363616837No Hit
TTCACAGTGGCTAAGTTCTGC153920.13331099935267257No Hit
CAAAGAATTCTCCTTTTGGGCTT153920.13331099935267257No Hit
CAACACCAGTCGATGGGCTGT153440.13289526858546047No Hit
ACTGGACTTGGAGTCAGAAGGCT153210.1326960642595047No Hit
TCAGGCTCAGTCCCCTCCCGATA152940.1324622157029479No Hit
GGAATACCGGGTGCTGTAGGCTTT145500.1260183888111607No Hit
CAAAGTGCTGTTCGTGCAGGTAG145480.1260010666958602No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT140230.12145401142947812No Hit
AGCAGCATTGTACAGGGCTATG138880.12028476864669416No Hit
CCCCCACTGCTAAATTTGACTGGCTA136490.11821477586828402No Hit
AACATTCAACGCTGTCGGTGAGA134990.11691561722074628No Hit
ACTGGACTTGGAGTCAGAAGG132300.1145857927128286No Hit
CTCCCACTGCTTCACTTGACTAGCCT132070.11438658838687282No Hit
TGAGAACTGAATTCCATAGGCTGT127700.11060170619371287No Hit
TCAGGCTCAGTCCCCTCCCGAAA126510.10957104033333294No Hit
CCAAGTCCTTCTGATCGAGGCCC123550.10700736726885844No Hit
ACCACAGGGTAGAACCACGGAC123530.10699004515355795No Hit
CACTAGATTGTGAGCTCCTGGA121310.10506729035520211No Hit
AGAGGTAGTAGGTTGCATAGTT119140.10318784084509751No Hit
TGAGAACTGAATTCCATGGGTT118250.10241700671422511No Hit
TTCACCACCTTCTCCACCCAGC118210.1023823624836241No Hit
TGAGGTAGTAGGTTGTATAGT117960.10216583604236783No Hit
TATTGCACTTGTCCCGGCCTGTAAT117130.10144696825739694No Hit
CACTAGATTGTGAGCTCCTGGAA115790.10028638653226322No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)