FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11766928
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG9605468.163099153831824No Hit
CCCCCCACTGCTAAATTTGACTG6036835.130336482045271No Hit
AACATTCAACGCTGTCGGTGAGT4802864.081660056048614No Hit
AAGCTGCCAGTTGAAGAACTGT4309333.6622387763399247No Hit
TTCAAGTAATCCAGGATAGGCT3966663.3710242809338173No Hit
TAGCAGCACGTAAATATTGGCG3618883.0754671057730616No Hit
AACATTCAACGCTGTCGGTGAG2888772.454990801337443No Hit
TCGTACGACTCTTAGCGG2315361.9676843437811466No Hit
CCCCCACTGCTAAATTTGACTG2111961.7948269930775478No Hit
TATTGCACTTGTCCCGGCCTGTAT2110371.793475748300661No Hit
TATTGCACTTGTCCCGGCCTGTAA2052801.7445504893035801No Hit
TATTGCACTTGTCCCGGCCTGT1855451.5768346674680085No Hit
TAGCTTATCAGACTGATGTTGAC1515851.2882291792726188No Hit
TAGCTTATCAGACTGATGTTGACT1477311.2554763656240608No Hit
CATAAAGTAGAAAGCACTACT1319251.121150737048786No Hit
TGAGGTAGTAGGTTGTATAGTT1208001.0266060946408442No Hit
TAGCTTATCAGACTGATGTTGA1121780.9533329344753363No Hit
ATCCCGGACGAGCCCCCA996430.8468055553667024No Hit
TATTGCACTTGTCCCGGCCTGTA992430.8434061974374281No Hit
TGTAAACATCCTTGACTGGAAGCT868010.7376691690473504No Hit
TGAGGTAGTAGATTGTATAGTT862210.7327401000499026No Hit
CGCCTGTCTGAGCGTCGCT786460.6683647592642702No Hit
CCCATAAAGTAGAAAGCACTAC759120.6451301478176802No Hit
AGCAGCATTGTACAGGGCTATGA754900.6415438252022958No Hit
ACTGGACTTGGAGTCAGAAGGC721630.6132696656255566No Hit
GCCTGTCTGAGCGTCGCT702810.597275686568321No Hit
TTCAAGTAATTCAGGATAGGTT693280.5891767163018249No Hit
CCCATAAAGTAGAAAGCACT642410.5459453818362787No Hit
TGTAAACATCCCCGACTGGAAGCT619500.5264755592963601No Hit
CAACGGAATCCCAAAAGCAGCTGT612740.5207306443958866No Hit
CATTGCACTTGTCTCGGTCTGA583740.4960852994086477No Hit
CACCCGGCTGTGTGCACATGTGC550330.46769216230438393No Hit
CAACGGAATCCCAAAAGCAGCT395440.33606052488805915No Hit
CAACACCAGTCGATGGGCTGT395210.33586506180712583No Hit
TGAGGTAGTAGTTTGTACAGTT370620.314967508936912No Hit
TGTAAACATCCTACACTCTCAGCT354080.3009111638993627No Hit
TTAATGCTAATCGTGATAGGGGTT322690.2742347025493824No Hit
AACATTCAACGCTGTCGGTGAGTT318280.2704869104323575No Hit
CACTAGATTGTGAGCTCCTGGA315940.26849828604373205No Hit
TATTGCACTTGTCCCGGCCTGTT313650.2665521536292225No Hit
AACCGGGCGGAAACACCA311860.26503094095587226No Hit
TTCTCACTACTGCACTTGACTAG284100.2414393969267085No Hit
TGTCAGTTTGTCAAATACCCCAA283110.2405980558392131No Hit
CTACGCCTGTCTGAGCGTCGCT277010.23541403499706975No Hit
AACGGAATCCCAAAAGCAGCTG268390.2280884186594836No Hit
AGCTCGGTCTGAGGCCCCTCAGT252860.21489041149907606No Hit
TACGCCTGTCTGAGCGTCGCT251500.21373462980312277No Hit
CACTAGATTGTGAGCTCCTGGAA249850.21233239465729714No Hit
ATCCCACCGCTGCCACCA247250.21012281200326882No Hit
TATTGCACTTGTCCCGGCCTGTAG244380.2076837726890145No Hit
ACCGGGTGCTGTAGGCTT243750.2071483738151538No Hit
ACCGGGTGCTGTAGGCTTA234320.1991343874968896No Hit
TGAGGTAGTAGTTTGTGCTGTT225240.1914178449974369No Hit
CTCCCACTGCTTCACTTGACTAG221300.18806947743710167No Hit
TCGTTTCCCGGCCAATGCACCA221080.1878825127509916No Hit
CTTCTCACTACTGCACTTGACTAG218110.18535848948850542No Hit
TTCAAGTAATCCAGGATAGGCTT217520.18485708419393745No Hit
ACGCCTGTCTGAGCGTCGCT208210.17694507861355147No Hit
TCGTTTCCCGGGCGGCGCACCA206960.17588277926065324No Hit
TCCTGTCTGAGCGTCGCT198220.16845518218518887No Hit
TATTGCACTTGTCCCGGCCTGTTT198150.16839569342142655No Hit
CAAAGAATTCTCCTTTTGGGCT196420.16692547111701542No Hit
TTCAAGTAATCCAGGATAGGC193730.16463940290957843No Hit
ACCGGGTGCTGTAGGCTTT192340.1634581260291556No Hit
TAGCAGCACGTAAATATTGGC189040.16065365573750431No Hit
TTATAAAGCAATGAGACTGATT187570.159404391698496No Hit
CCCATAAAGTAGAAAGCACTA186240.15827410518701227No Hit
TGAGAACTGAATTCCATGGGTT185940.1580191533423167No Hit
TGTCAGTTTGTCAAATACCCCA185760.15786618223549936No Hit
ACCACAGGGTAGAACCACGGAC184140.15648944227414327No Hit
TGTAAACATCCCCGACTGGAAGC183530.1559710401899289No Hit
TTCACAGTGGCTAAGTTCCG182800.15535065736783635No Hit
ACTGGACTTGGAGTCAGAAGGCT182760.1553166637885436No Hit
ACTGGACTTGGAGTCAGAAGGCA182540.15512969910243352No Hit
TTCACCACCTTCTCCACCCAGC180160.15310708113451532No Hit
TAGCTTATCAGACTGATGTTGACA174040.1479060635027256No Hit
TATTGCACTTGTCCCGGCCTGA173680.14760012128909092No Hit
AGCAGCATTGTACAGGGCTATGT168380.14309597203280244No Hit
CCCCCACTGCTAAATTTGACTGGCTT162690.13826038537840973No Hit
ACTGGACTTGGAGTCAGAAGG162010.1376824945304331No Hit
TTCACAGTGGCTAAGTTCCGC161580.13731706355303608No Hit
CAAAGAATTCTCCTTTTGGGCTT161260.13704511491869417No Hit
CAAAGTGCTGTTCGTGCAGGT158000.1342746382063356No Hit
TTCACAGTGGCTAAGTTCTGC156920.13335681156543153No Hit
TGAGGTAGTAGGTTGTGTGGTT152000.12917560131242412No Hit
AACATTCAACGCTGTCGGTGA148610.12629464546736413No Hit
CCCCCCACTGCTAAATTTGACTGGCT147020.12494340069047757No Hit
TGTAAACATCCTACACTCAGCT144100.12246186940210732No Hit
CCCCCCACTGCTAAATTTGACTGG143160.12166302028872786No Hit
TATTGCACTTGTCCCGGCCTGTAAT141180.11998033811373707No Hit
CAAAGTGCTGTTCGTGCAGGTAG133710.1136320371808173No Hit
AGCAGCATTGTACAGGGCTATG133670.11359804360152453No Hit
TTCACAGTGGCTAAGTTCTG129630.11016469209295747No Hit
AATGACACGATCACTCCCGTTG125930.10702028600837873No Hit
TGAGAACTGAATTCCATAGGCTGT119780.10179377319211946No Hit
TGAGGTAGTAGGTTGTATAGT119540.101589811716363No Hit
AACATTCAACGCTGTCGGTGAGA118460.10067198507545895No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)