FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7337324
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG6127368.35094647585414No Hit
CAACGGAATCCCAAAAGCAGCTG5293097.213924313550827No Hit
AACATTCAACGCTGTCGGTGAGT2560983.4903460716740873No Hit
AAGCTGCCAGTTGAAGAACTGT2119682.8889006400698674No Hit
TTCAAGTAATCCAGGATAGGCT1987292.7084670105886013No Hit
TAGCAGCACGTAAATATTGGCG1982362.7017479397120803No Hit
TAGCTTATCAGACTGATGTTGAC1554952.119233115506416No Hit
CCCCCACTGCTAAATTTGACTG1546672.1079483473811433No Hit
AACATTCAACGCTGTCGGTGAG1496262.0392448254976885No Hit
TATTGCACTTGTCCCGGCCTGTAA1238161.6874817031386373No Hit
TAGCTTATCAGACTGATGTTGACT1083551.476764553398487No Hit
TATTGCACTTGTCCCGGCCTGTAT999381.3620497064052235No Hit
TTAATGCTAATCGTGATAGGGGTT947831.2917924845624917No Hit
TGTAAACATCCTTGACTGGAAGCT741421.0104773893043295No Hit
AGCAGCATTGTACAGGGCTATGA722380.9845278741950063No Hit
TGAGGTAGTAGGTTGTATAGTT598250.8153517549449908No Hit
TATTGCACTTGTCCCGGCCTGT558760.7615310431977652No Hit
TATTGCACTTGTCCCGGCCTGTA548050.7469344409487709No Hit
ACTGGACTTGGAGTCAGAAGGC509180.6939587239162398No Hit
TAGCTTATCAGACTGATGTTGA481270.6559203328079829No Hit
CGCCTGTCTGAGCGTCGCT457380.623360778398228No Hit
TGTAAACATCCCCGACTGGAAGCT455810.6212210337174697No Hit
TTCAAGTAATTCAGGATAGGTT399490.544462804150396No Hit
TGAGGTAGTAGATTGTATAGTT356340.4856538977970715No Hit
CAACGGAATCCCAAAAGCAGCTGT349530.4763725848824449No Hit
TGTAAACATCCTACACTCTCAGCT333700.4547979617637166No Hit
AACATTCAACGCTGTCGGTGAGTT283140.3858900056750935No Hit
CAACGGAATCCCAAAAGCAGCT274620.3742781428215518No Hit
TAGCTTATCAGACTGATGTTGACA228120.31090353921947567No Hit
GCGTTCGCGCTTTCCCCTG227420.3099495129286917No Hit
CAAAGAATTCTCCTTTTGGGCT216380.2949031554283278No Hit
CATTGCACTTGTCTCGGTCTGA212070.28902907926650095No Hit
CATAAAGTAGAAAGCACTACT202320.2757408559305818No Hit
CCCCCACTGCTAAATTTGACTGGCTT202260.27565908224851454No Hit
CTACGCCTGTCTGAGCGTCGCT202080.275413761202313No Hit
TCGTACGACTCTTAGCGG201020.2739690928191259No Hit
CCCATAAAGTAGAAAGCACTAC199730.2722109586546812No Hit
TACGCCTGTCTGAGCGTCGCT190420.25952240898725476No Hit
CACCCGGCTGTGTGCACATGTGC184070.25086802763514326No Hit
CACTAGATTGTGAGCTCCTGGAA165170.2251093177839768No Hit
TCGTTTCCCGGCCAATGCACCA154090.21000844449556816No Hit
CCCCCCACTGCTAAATTTGACTGGCT152900.20838659980123544No Hit
AGCAGCATTGTACAGGGCTATGT145310.19804222901973526No Hit
TATTGCACTTGTCCCGGCCTGTAG144710.19722449219906332No Hit
CACTAGATTGTGAGCTCCTGGA144530.1969791711528617No Hit
TGAGGTAGTAGTTTGTACAGTT143180.1951392633063498No Hit
AGCTCGGTCTGAGGCCCCTCAGT140600.19162299497746044No Hit
GGAATACCGGGTGCTGTAGGCTTT131290.178934445310034No Hit
TGTCAGTTTGTCAAATACCCCAA130420.1777487269200597No Hit
CTTCTCACTACTGCACTTGACTAG129560.17657663747709657No Hit
TAGCAGCACGTAAATATTGGC129240.17614051117273818No Hit
TTCAAGTAATCCAGGATAGGCTT129210.1760996243317046No Hit
CCCCCCACTGCTAAATTTGACTGG126870.172910450731084No Hit
CAAAGAATTCTCCTTTTGGGCTT126640.1725969849498264No Hit
TTCTCACTACTGCACTTGACTAG124700.16995296922965375No Hit
TTCACAGTGGCTAAGTTCCG124670.16991208238862016No Hit
TATTGCACTTGTCCCGGCCTGTT121190.16516920882872285No Hit
ATCACATTGCCAGGGATTTCCA120660.1644468746371293No Hit
TTAATGCTAATCGTGATAGGGGTTT119670.16309760888302058No Hit
AACGGAATCCCAAAAGCAGCTG119010.16219809838028143No Hit
CAAAGTGCTGTTCGTGCAGGTAG116570.15887263530954882No Hit
CTCCCACTGCTTCACTTGACTAG116490.15876360373345924No Hit
ACCCCCCACTGCTAAATTTGACTG115410.15729167745624972No Hit
TATTGCACTTGTCCCGGCCTGTAAA113230.1543205670078083No Hit
CAACACCAGTCGATGGGCTGT112520.15335291177001317No Hit
GGGCTACGCCTGTCTGAGCGTCGCT110640.15079066973190772No Hit
CAACGGAATCCCAAAAGCAGCTGA110340.15038180132157172No Hit
CCCATAAAGTAGAAAGCACT109220.14885535925631743No Hit
TCGTTTCCCGGGCGGCGCACCA108990.14854189347505983No Hit
TGTCAGTTTGTCAAATACCCCA108870.14837834611092546No Hit
TATTGCACTTGTCCCGGCCTGTAAT106680.14539360671547283No Hit
TGTAAACATCCTACACTCAGCT105180.14334926466379297No Hit
GAATACCGGGTGCTGTAGGCTTT104660.1426405594192106No Hit
ACCACAGGGTAGAACCACGGAC103750.14140032524119148No Hit
TGAGGTAGTAGTTTGTGCTGTT103570.14115500419498989No Hit
CCTCGACACAAGGGTTTGT103070.14047355684442991No Hit
AGCAGCATTGTACAGGGCTATG101740.13866090689194044No Hit
TGAGGTAGTAGGTTGTGTGGTT100970.13761147797207812No Hit
ACTGGACTTGGAGTCAGAAGGCA98320.13399980701411032No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT95240.12980209133466097No Hit
ACTGGACTTGGAGTCAGAAGGCT94400.12865725978572023No Hit
CCCCCCACTGCTAAATTTGACTGTA92430.125972357224514No Hit
GCCTGTCTGAGCGTCGCT91560.12478663883453966No Hit
ATCACATTGCCAGGGATTTCC90150.12286495730596059No Hit
CGCGACCTCAGATCAGACGTGGCG90060.1227422967828598No Hit
ACGCCTGTCTGAGCGTCGCT89520.12200633364425505No Hit
CTGCGTTCGCGCTTTCCCCTG89370.12180189943908706No Hit
TTATAAAGCAATGAGACTGATT88430.12052077842003434No Hit
GGAATACCGGGTGCTGTAGGCTT86640.11808119690502968No Hit
TTCAAGTAATCCAGGATAGGC86600.11802668111698489No Hit
AACATTCATTGCTGTCGGTGGGTT84330.11493291014544266No Hit
TGTAAACATCCCCGACTGGAAGC82750.1127795365176732No Hit
TGAGGGGCAGAGAGCGAGACTTT79640.10854093399719025No Hit
TGATGTAGTAGGTTGTATAGTT79040.10772319717651829No Hit
ACCGGGTGCTGTAGGCTT78350.10678279983274555No Hit
ATGCACCTGGGCAAGGATTCTGA78310.10672828404470076No Hit
CTCCCACTGCTTCACTTGACTAGCCT76760.1046157972579649No Hit
TATTGCACTTGTCCCGGCCTGTTT74780.10191726574974745No Hit
TTAATGCTAATCGTGATAGGGGT74230.1011676736641315No Hit
CCCGCCACTGCTAAATTTGACTG73580.10028179210840354No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)