FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5914326
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG4874828.242393131525045No Hit
CCCCCCACTGCTAAATTTGACTG3035605.132622043492361No Hit
AACATTCAACGCTGTCGGTGAGT2688134.54511638350676No Hit
AAGCTGCCAGTTGAAGAACTGT2101323.5529323206059322No Hit
TTCAAGTAATCCAGGATAGGCT1978933.3459941166584324No Hit
TAGCAGCACGTAAATATTGGCG1825153.0859813950059567No Hit
GCCTGTCTGAGCGTCGCT1638372.7701719519688295No Hit
AACATTCAACGCTGTCGGTGAG1272912.152248624779899No Hit
CCCCCACTGCTAAATTTGACTG1089571.842255567244687No Hit
CGCCTGTCTGAGCGTCGCT834131.4103551275327062No Hit
TATTGCACTTGTCCCGGCCTGT685331.158762638380096No Hit
TGAGGTAGTAGGTTGTATAGTT624891.0565700977592374No Hit
TATTGCACTTGTCCCGGCCTGTAA621661.0511087823024974No Hit
TAGCTTATCAGACTGATGTTGAC610901.032915669511623No Hit
TATTGCACTTGTCCCGGCCTGTAT570470.9645562317667306No Hit
TCCTGTCTGAGCGTCGCT566580.9579789818822972No Hit
TAGCTTATCAGACTGATGTTGA493220.8339411794344782No Hit
CATAAAGTAGAAAGCACTACT490550.8294267174315383No Hit
AGCAGCATTGTACAGGGCTATGA482980.816627287707847No Hit
TAGCTTATCAGACTGATGTTGACT449990.7608474744205849No Hit
TGTAAACATCCTTGACTGGAAGCT397680.6724012169772177No Hit
ACTGGACTTGGAGTCAGAAGGC343920.5815032854124037No Hit
TATTGCACTTGTCCCGGCCTGTA343540.5808607777116108No Hit
TGAGGTAGTAGATTGTATAGTT315860.5340591641380608No Hit
TTCAAGTAATTCAGGATAGGTT291110.49221162309957217No Hit
TGTAAACATCCCCGACTGGAAGCT282690.4779750050977914No Hit
CCCATAAAGTAGAAAGCACTAC273920.4631466036873855No Hit
CAACGGAATCCCAAAAGCAGCTGT273810.4629606146161033No Hit
CCCATAAAGTAGAAAGCACT237080.40085717290524736No Hit
CATTGCACTTGTCTCGGTCTGA236280.3995045251141043No Hit
AACCGGGCGGAAACACCA224070.37885973820178326No Hit
AACATTCAACGCTGTCGGTGAGTT222310.3758839130612685No Hit
TACGCCTGTCTGAGCGTCGCT221890.3751737729709184No Hit
ACGCCTGTCTGAGCGTCGCT203200.34357253895033857No Hit
CTACGCCTGTCTGAGCGTCGCT196930.3329711618872548No Hit
TCGTACGACTCTTAGCGG195550.33063784444753297No Hit
GCGTTCGCGCTTTCCCCTG186320.31503167055721987No Hit
TGTAAACATCCTACACTCTCAGCT184240.31151478630024787No Hit
TGTCAGTTTGTCAAATACCCCAA183410.310111414216937No Hit
CACCCGGCTGTGTGCACATGTGC178530.3018602626909643No Hit
TTCTCACTACTGCACTTGACTAG178300.30147137645101063No Hit
TGAGGTAGTAGTTTGTACAGTT154970.262024785241801No Hit
CAACACCAGTCGATGGGCTGT151700.25649583739550375No Hit
ATCCCGGACGAGCCCCCA147010.24856593971992752No Hit
ATCCCACCGCTGCCACCA142300.24060222584957272No Hit
CTCCCACTGCTTCACTTGACTAG135300.22876655767707088No Hit
CCCCCACTGCTAAATTTGACTGGCTT132930.22475933859580957No Hit
CTTCTCACTACTGCACTTGACTAG130540.22071830331976966No Hit
TTCACAGTGGCTAAGTTCCG129710.21931493123645873No Hit
ACCGGGTGCTGTAGGCTT126260.21348163763715428No Hit
AACGGAATCCCAAAAGCAGCTG120610.20392856261220635No Hit
TGAGGTAGTAGTTTGTGCTGTT118210.1998706192387772No Hit
CAACGGAATCCCAAAAGCAGCT111130.18789968628716106No Hit
TATTGCACTTGTCCCGGCCTGTT109100.1844673425171355No Hit
TGTCAGTTTGTCAAATACCCCA108490.18343594857638892No Hit
TGTAAACATCCTACACTCAGCT104770.17714613634757367No Hit
ACCACAGGGTAGAACCACGGAC101720.17198916664384073No Hit
TTCAAGTAATCCAGGATAGGC101500.17161718850127639No Hit
AGCAGCATTGTACAGGGCTATG99580.16837083380253304No Hit
AGCAGCATTGTACAGGGCTATGT99520.16826938521819731No Hit
TTCAAGTAATCCAGGATAGGCTT97910.16554718153852188No Hit
CCCCCCACTGCTAAATTTGACTGGCT97790.16534428436985044No Hit
TAGCAGCACGTAAATATTGGC95620.16167522723637487No Hit
TGAGGTAGTAGGTTGTGTGGTT95540.16153996245726057No Hit
AACATTCAACGCTGTCGGTGA93780.15856413731674582No Hit
TAGCAGCACGTAAATATTGGCGT91780.15518251783888815No Hit
CACTAGATTGTGAGCTCCTGGA89480.15129365543935183No Hit
AGCTCGGTCTGAGGCCCCTCAGT88360.14939994853175156No Hit
TGTAAACATCCCCGACTGGAAGC87670.14823328981189066No Hit
TTATAAAGCAATGAGACTGATT86820.14679610153380115No Hit
ACTGGACTTGGAGTCAGAAGGCA86630.14647484768340469No Hit
TGATGTAGTAGGTTGTATAGTT86210.14576470759305454No Hit
TCGTTTCCCGGCCAATGCACCA84180.14233236382302902No Hit
CCCCCCACTGCTAAATTTGACTGG83440.1410811646162217No Hit
ACTGGACTTGGAGTCAGAAGG83120.14054010549976448No Hit
CAAAGTGCTGTTCGTGCAGGT80030.13531550340647439No Hit
TACCTGGTTGATCCTGCC79560.13452082282917782No Hit
TTCACCACCTTCTCCACCCAGC78210.1322382296816239No Hit
GTCAGGATGGCCGAGCGGTCTAAGG76550.12943148551500205No Hit
CAAAGAATTCTCCTTTTGGGCT75740.1280619296264697No Hit
AACATTCAACGCTGTCGGTGAGA72570.1227020627540653No Hit
ACTGGACTTGGAGTCAGAAGGCT70730.11959097283443625No Hit
ACCGGGTGCTGTAGGCTTT69790.11800161167984315No Hit
TAGCTTATCAGACTGATGTTGACA69450.11742673636860736No Hit
TCGTTCGCGCTTTCCCCTG69410.1173591039790502No Hit
CACTAGATTGTGAGCTCCTGGAA68480.11578665092184638No Hit
TGAGGTAGTAGGTTGTATAGT67950.1148905217602141No Hit
TTCACAGTGGCTAAGTTCTG67800.11463690029937477No Hit
AATGACACGATCACTCCCGTTG67580.11426492215681043No Hit
CAAAGTGCTGTTCGTGCAGGTAG66790.11292918246305665No Hit
GGAATACCGGGTGCTGTAGGCTTT66770.11289536626827809No Hit
TATTGCACTTGTCCCGGCCTGTAG66590.11259102051527088No Hit
CCTCGACACAAGGGTTTGT66570.11255720432049232No Hit
TTCACAGTGGCTAAGTTCTGC66510.11245575573615657No Hit
CCCTGTCTGAGCGTCGCT66330.11215140998314939No Hit
ACCCCCCACTGCTAAATTTGACTG66180.11189778852231007No Hit
CCCATAAAGTAGAAAGCACTA63450.10728187793503435No Hit
ACCGGGTGCTGTAGGCTTA62260.10526981434570905No Hit
TATTGCACTTGTCCCGGCCTGA61570.10410315562584815No Hit
CAAAGAATTCTCCTTTTGGGCTT59420.10046791468715116No Hit
GGAATACCGGGTGCTGTAGGCTT59290.10024810942109041No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)