FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9703670
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG8710588.976583086605377No Hit
CCCCCCACTGCTAAATTTGACTG6055516.240432743487774No Hit
TTCAAGTAATCCAGGATAGGCT3086113.180353412677884No Hit
AAGCTGCCAGTTGAAGAACTGT2673432.75507101952148No Hit
AACATTCAACGCTGTCGGTGAGT2426072.5001571570344003No Hit
TAGCAGCACGTAAATATTGGCG2349602.4213519214894985No Hit
CCCCCACTGCTAAATTTGACTG1901201.959258713455837No Hit
TAGCTTATCAGACTGATGTTGAC1797871.852773229097857No Hit
TAGCTTATCAGACTGATGTTGACT1469791.514674344861274No Hit
TATTGCACTTGTCCCGGCCTGTAA1285041.3242824622024452No Hit
AACATTCAACGCTGTCGGTGAG1207851.244735239347587No Hit
TAGCTTATCAGACTGATGTTGA1205231.242035229969692No Hit
TATTGCACTTGTCCCGGCCTGTAT981011.0109680151942513No Hit
TGAGGTAGTAGGTTGTATAGTT867320.89380615787635No Hit
AGCAGCATTGTACAGGGCTATGA715990.7378548528546416No Hit
ACTGGACTTGGAGTCAGAAGGC700670.7220670117594683No Hit
TGTAAACATCCTTGACTGGAAGCT696680.7179551654167959No Hit
TATTGCACTTGTCCCGGCCTGT687460.708453605697638No Hit
CATAAAGTAGAAAGCACTACT641680.6612755792396073No Hit
CAACGGAATCCCAAAAGCAGCTGT625400.6444984217311595No Hit
CCCATAAAGTAGAAAGCACTAC580230.5979490234107302No Hit
TGAGGTAGTAGATTGTATAGTT553360.5702584692183473No Hit
TTCAAGTAATTCAGGATAGGTT530540.5467415936444665No Hit
CCCCCACTGCTAAATTTGACTGGCTT499790.5150525522817656No Hit
TATTGCACTTGTCCCGGCCTGTA462860.47699478650860966No Hit
TGTAAACATCCCCGACTGGAAGCT416770.4294972932921255No Hit
CACCCGGCTGTGTGCACATGTGC407460.41990298515922325No Hit
TGTAAACATCCTACACTCTCAGCT350790.3615024006381091No Hit
CGCCTGTCTGAGCGTCGCT321320.33113244782644097No Hit
CATTGCACTTGTCTCGGTCTGA318580.32830877389688645No Hit
CGCGACCTCAGATCAGACGTGGCG314660.3242690652093486No Hit
CCCCCCACTGCTAAATTTGACTGGCT309340.31878660341911874No Hit
AACCGGGCGGAAACACCA283450.2921059763986203No Hit
CTCCCACTGCTTCACTTGACTAG282870.2915082643989336No Hit
CAAAGAATTCTCCTTTTGGGCT272270.28058456233569357No Hit
TTCTCACTACTGCACTTGACTAG265790.2739066765460903No Hit
GCCTGTCTGAGCGTCGCT253790.2615402213801582No Hit
CTTCTCACTACTGCACTTGACTAG249220.2568306630377991No Hit
CGCGACCTCAGATCAGACGTGG243320.2507504892478825No Hit
CAACACCAGTCGATGGGCTGT239730.24705085807740784No Hit
CCCATAAAGTAGAAAGCACT237900.24516497366460316No Hit
CACTAGATTGTGAGCTCCTGGAA232960.24007411628796113No Hit
CACTAGATTGTGAGCTCCTGGA232730.23983709256394747No Hit
AGCTCGGTCTGAGGCCCCTCAGT229790.2368073110482941No Hit
CAAAGAATTCTCCTTTTGGGCTT225920.232819129257281No Hit
AACATTCAACGCTGTCGGTGAGTT222730.22953171325900407No Hit
CTACGCCTGTCTGAGCGTCGCT220800.22754277505315No Hit
TAGCTTATCAGACTGATGTTGACA219980.22669773395014464No Hit
AACGGAATCCCAAAAGCAGCTG209420.21581525340412444No Hit
CCCCCCACTGCTAAATTTGACTGG209080.21546487050775634No Hit
CAACGGAATCCCAAAAGCAGCT202120.20829232651151577No Hit
ACCGGGTGCTGTAGGCTTA202020.20818927271846632No Hit
TACGCCTGTCTGAGCGTCGCT194310.20024382527435497No Hit
ACCGGGTGCTGTAGGCTT188680.19444189672567183No Hit
TGAGGTAGTAGTTTGTACAGTT186610.19230868320954855No Hit
TTCAAGTAATCCAGGATAGGCTT183400.1890006564526617No Hit
ACTGGACTTGGAGTCAGAAGGCA182820.188402944452975No Hit
TCGTACGACTCTTAGCGG178260.18370369148992083No Hit
ACCCCCCACTGCTAAATTTGACTG173900.1792105461129655No Hit
ACCGGGTGCTGTAGGCTTT158470.16330934584543785No Hit
CGCGACCTCAGATCAGACGTGGC154770.1594963555026088No Hit
ACTGGACTTGGAGTCAGAAGGCT153410.1580948239171365No Hit
CTCCCACTGCTTCACTTGACTAGCCT152190.1568375676419334No Hit
TGAGGTAGTAGTTTGTGCTGTT137450.14164743854644685No Hit
TATTGCACTTGTCCCGGCCTGTAG136400.14056537371942782No Hit
AGCAGCATTGTACAGGGCTATGT133070.13713368241088164No Hit
TAGCAGCACGTAAATATTGGC131490.1355054324807006No Hit
ACTGGACTTGGAGTCAGAAGG125350.12917792958746535No Hit
TTCACAGTGGCTAAGTTCCGC124780.1285905229670836No Hit
ACCACAGGGTAGAACCACGGAC123640.12741570972632005No Hit
CAAAGTGCTGTTCGTGCAGGTAG123630.1274054043470151No Hit
TGTAAACATCCCCGACTGGAAGC122070.12579776517544392No Hit
ATCACATTGCCAGGGATTTCCA120610.1242931797969222No Hit
TATTGCACTTGTCCCGGCCTGTT118830.12245882228064228No Hit
CCCCCACTGCTAAATTTGACTGGCT118130.12173744572929623No Hit
TCGTTTCCCGGGCGGCGCACCA116170.11971759138552733No Hit
TTCAAGTAATCCAGGATAGGC114250.1177389585589782No Hit
TATTGCACTTGTCCCGGCCTGTAAT113600.11706910890415688No Hit
TGTCAGTTTGTCAAATACCCCA113240.11669811524917892No Hit
TTCACAGTGGCTAAGTTCCG106930.11019542090775965No Hit
TGTAAACATCCTACACTCAGCT106870.11013358863192999No Hit
ATCACATTGCCAGGGATTTCC105620.10884541621881205No Hit
GGAATACCGGGTGCTGTAGGCTTT102590.10572288628941422No Hit
ATGCACCTGGGCAAGGATTCTGA102570.10570227553080433No Hit
ACGCCTGTCTGAGCGTCGCT101190.10428013318672213No Hit
CAGTGCAATAGTATTGTCAAAGC100750.10382669649730462No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)