FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7362027
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG4889666.641730599466696No Hit
CCCCCCACTGCTAAATTTGACTG3949085.36412050648551No Hit
AAGCTGCCAGTTGAAGAACTGT2491373.3840815851395276No Hit
TTCAAGTAATCCAGGATAGGCT2007582.7269391975878383No Hit
AACATTCAACGCTGTCGGTGAGT1963982.6677163775682975No Hit
TAGCAGCACGTAAATATTGGCG1860402.5270214303750858No Hit
TAGCTTATCAGACTGATGTTGAC1495822.0318045560006777No Hit
TAGCTTATCAGACTGATGTTGACT1208391.641382189986535No Hit
CCCCCACTGCTAAATTTGACTG1153601.5669597517096856No Hit
TATTGCACTTGTCCCGGCCTGTAA937721.2737252933193535No Hit
AACATTCAACGCTGTCGGTGAG874031.187213793157781No Hit
TGAGGTAGTAGGTTGTATAGTT832111.1302729533591767No Hit
TAGCTTATCAGACTGATGTTGA824051.1193248815849222No Hit
TGTAAACATCCTTGACTGGAAGCT773911.0512186385624502No Hit
TATTGCACTTGTCCCGGCCTGTAT734350.9974834376456375No Hit
TATTGCACTTGTCCCGGCCTGT617210.8383696500977246No Hit
GCCTGTCTGAGCGTCGCT606650.824025774423267No Hit
AGCAGCATTGTACAGGGCTATGA586600.7967914271436385No Hit
CGCCTGTCTGAGCGTCGCT554670.7534202197302455No Hit
TGAGGTAGTAGATTGTATAGTT491520.6676422132111169No Hit
TGTAAACATCCCCGACTGGAAGCT441340.5994816373262418No Hit
ACTGGACTTGGAGTCAGAAGGC402030.5460860167994493No Hit
CATAAAGTAGAAAGCACTACT382950.5201692414331No Hit
TTCAAGTAATTCAGGATAGGTT374680.5089359221312283No Hit
CAACGGAATCCCAAAAGCAGCTGT359570.4884116833584012No Hit
TATTGCACTTGTCCCGGCCTGTA356630.48441821797176243No Hit
CCCCCACTGCTAAATTTGACTGGCTT344840.4684036067784049No Hit
CCCATAAAGTAGAAAGCACTAC295120.4008678588111671No Hit
TTCTCACTACTGCACTTGACTAG263200.35751023461337483No Hit
TGTAAACATCCTACACTCTCAGCT243410.3306290509393677No Hit
ACCGGGTGCTGTAGGCTT241110.32750491135118087No Hit
TTAATGCTAATCGTGATAGGGGTT230150.3126177070526908No Hit
CTACGCCTGTCTGAGCGTCGCT221390.3007188101863794No Hit
TGTCAGTTTGTCAAATACCCCAA216410.29395436881717496No Hit
TGAGGTAGTAGTTTGTACAGTT215220.2923379661606783No Hit
CCCCCCACTGCTAAATTTGACTGGCT205840.2795969099271165No Hit
TAGCTTATCAGACTGATGTTGACA203890.27694818288495815No Hit
CGCGACCTCAGATCAGACGTGGCG199380.2708221526489919No Hit
TACGCCTGTCTGAGCGTCGCT192820.26191156321485914No Hit
CTCCCACTGCTTCACTTGACTAG189030.2567635245021514No Hit
CACCCGGCTGTGTGCACATGTGC188750.25638319446532865No Hit
AACATTCAACGCTGTCGGTGAGTT185720.25226748013828254No Hit
CTTCTCACTACTGCACTTGACTAG185680.2522131472758793No Hit
ACCGGGTGCTGTAGGCTTA173540.2357231235364934No Hit
ACCGGGTGCTGTAGGCTTT171750.23329172794394806No Hit
GTCAGGATGGCCGAGCGGTCTAAGG166700.22643220406553793No Hit
CGCGACCTCAGATCAGACGTGG166190.2257394600698965No Hit
CAAAGAATTCTCCTTTTGGGCT164180.22300923373413328No Hit
CATTGCACTTGTCTCGGTCTGA161990.22003450951755543No Hit
GCGTTCGCGCTTTCCCCTG160960.21863543831067178No Hit
GGAATACCGGGTGCTGTAGGCTTT160810.21843169007665958No Hit
CCCCCCACTGCTAAATTTGACTGG150860.20491639055385152No Hit
ACGCCTGTCTGAGCGTCGCT148730.20202316563087855No Hit
TGAGGTAGTAGTTTGTGCTGTT147680.2005969279927933No Hit
GGGCTACGCCTGTCTGAGCGTCGCT144040.1956526375140977No Hit
ACCCCCCACTGCTAAATTTGACTG142720.19385965305479047No Hit
TGTCAGTTTGTCAAATACCCCA137840.18723104384159417No Hit
CAAAGAATTCTCCTTTTGGGCTT137100.18622588588713407No Hit
CAACACCAGTCGATGGGCTGT130130.17675838461336804No Hit
GGAATACCGGGTGCTGTAGGCTT128540.1745986533328389No Hit
CCCATAAAGTAGAAAGCACT124530.16915178387691326No Hit
AACCGGGCGGAAACACCA123920.1683232077252637No Hit
TGTAAACATCCCCGACTGGAAGC123790.16814662592245316No Hit
TGATGTAGTAGGTTGTATAGTT122290.16610914358233134No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC115180.1564514772901539No Hit
AGCTCGGTCTGAGGCCCCTCAGT115180.1564514772901539No Hit
CTCCCACTGCTTCACTTGACTAGCCT114390.15537840325768976No Hit
TTCAAGTAATCCAGGATAGGCTT114340.1553104871796857No Hit
ACTGGACTTGGAGTCAGAAGGCA112550.15287909158714033No Hit
TGAGGTAGTAGGTTGTGTGGTT111870.1519554329262851No Hit
AACGGAATCCCAAAAGCAGCTG111360.15126268893064368No Hit
ATCCCGGACGAGCCCCCA109150.14826079828286423No Hit
TAGCAGCACGTAAATATTGGC103940.1411839429548411No Hit
TATTGCACTTGTCCCGGCCTGTAG103120.1400701192755745No Hit
TATTGCACTTGTCCCGGCCTGTT101470.13782888870144053No Hit
CAACGGAATCCCAAAAGCAGCT98590.13391692260840662No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT98580.1339033393928058No Hit
TCGTACGACTCTTAGCGG97740.1327623492823376No Hit
TGTAAACATCCTACACTCAGCT94800.12876888389569882No Hit
CAAAGTGCTGTTCGTGCAGGTAG94320.12811688954685985No Hit
AGCAGCATTGTACAGGGCTATGT93550.12707098194559732No Hit
TAGCAGCACGTAAATATTGGCGT92150.12516933176148362No Hit
GTGCGGTAACGCGACCGATCCCGGA92110.12511499889908037No Hit
ATCACATTGCCAGGGATTTCCA90920.12349859624258372No Hit
TTATAAAGCAATGAGACTGATT89270.12125736566844973No Hit
TTCACAGTGGCTAAGTTCCG88970.12084986920042537No Hit
ACCACAGGGTAGAACCACGGAC84870.1152807508040924No Hit
CCCCCACTGCTAAATTTGACTGGCT84580.11488683755166887No Hit
TTCACAGTGGCTAAGTTCCGC84290.1144929242992453No Hit
TTCAAGTAATCCAGGATAGGC83750.11375943065680144No Hit
CGCGACCTCAGATCAGACGTGGC83450.11335193418877708No Hit
CACTAGATTGTGAGCTCCTGGA82800.1124690251747243No Hit
GACCCGCGGGCGCTCTCCAGTCCT81070.1101191288757838No Hit
CACTAGATTGTGAGCTCCTGGAA80710.10963013311415457No Hit
TATTGCACTTGTCCCGGCCTGTAAT79860.10847555978808554No Hit
AGCAGCATTGTACAGGGCTATG79850.10846197657248473No Hit
TTCACAGTGGCTAAGTTCTGC79550.10805448010446037No Hit
ATCCCACCGCTGCCACCA76550.10397951542421673No Hit
ACTGGACTTGGAGTCAGAAGG75320.10230877990531685No Hit
GGGAATACCGGGTGCTGTAGGCTT74920.10176545128128436No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)