FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8871820
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG4558565.13824671826074No Hit
CCCCCCACTGCTAAATTTGACTG4510605.084187911837707No Hit
AAGCTGCCAGTTGAAGAACTGT2531162.8530335376506737No Hit
AACATTCAACGCTGTCGGTGAGT2131132.402133947713096No Hit
CGCCTGTCTGAGCGTCGCT2057362.3189830271579No Hit
TAGCTTATCAGACTGATGTTGAC1924012.168675649415791No Hit
CCCCCACTGCTAAATTTGACTG1791632.019461621178067No Hit
TAGCAGCACGTAAATATTGGCG1771531.9968056159840935No Hit
TTCAAGTAATCCAGGATAGGCT1504821.6961795888554998No Hit
TAGCTTATCAGACTGATGTTGACT1169151.3178243021161384No Hit
AACATTCAACGCTGTCGGTGAG1168401.3169789287880052No Hit
TATTGCACTTGTCCCGGCCTGTAA895571.0094546553018435No Hit
TAGCTTATCAGACTGATGTTGA890051.003232707606782No Hit
GCCTGTCTGAGCGTCGCT861720.9713001390920916No Hit
TGAGGTAGTAGGTTGTATAGTT847950.9557790847875632No Hit
TATTGCACTTGTCCCGGCCTGT836170.9425010877136822No Hit
ACCGGGTGCTGTAGGCTT831750.937519020899883No Hit
TATTGCACTTGTCCCGGCCTGTAT693540.7817336239914697No Hit
AGCAGCATTGTACAGGGCTATGA676700.7627521748637822No Hit
TGTAAACATCCTTGACTGGAAGCT610470.6881000741674199No Hit
ACCGGGTGCTGTAGGCTTT565760.6377045521662974No Hit
ACTGGACTTGGAGTCAGAAGGC516220.5818648259319958No Hit
ACCGGGTGCTGTAGGCTTA503610.5676512823749805No Hit
TATTGCACTTGTCCCGGCCTGTA490170.552502192334831No Hit
TACGCCTGTCTGAGCGTCGCT486030.5478357315635349No Hit
TGAGGTAGTAGATTGTATAGTT463300.5222152838989069No Hit
GCGTTCGCGCTTTCCCCTG428190.48264054049789107No Hit
CATAAAGTAGAAAGCACTACT418320.4715154274996562No Hit
TCGTACGACTCTTAGCGG345170.389063348895717No Hit
AACCGGGCGGAAACACCA343110.3867413901544441No Hit
CTACGCCTGTCTGAGCGTCGCT341110.3844870612794218No Hit
CAACACCAGTCGATGGGCTGT335260.3778931493199817No Hit
TTCAAGTAATTCAGGATAGGTT325650.3670610990754997No Hit
TGTAAACATCCCCGACTGGAAGCT324160.3653816240636082No Hit
ACGCCTGTCTGAGCGTCGCT317550.35793106713165956No Hit
CATTGCACTTGTCTCGGTCTGA278150.313520788293721No Hit
TCCTGTCTGAGCGTCGCT258860.2917777862941313No Hit
CACCCGGCTGTGTGCACATGTGC256120.2886893557353508No Hit
CAACGGAATCCCAAAAGCAGCTGT255780.28830611982659704No Hit
TTAATGCTAATCGTGATAGGGGTT229570.2587631399194303No Hit
CGCGACCTCAGATCAGACGTGG220250.24825796736182654No Hit
AACATTCAACGCTGTCGGTGAGTT211890.2388348726642335No Hit
CCTCGACACAAGGGTTTGT210640.23742591711734456No Hit
CCCATAAAGTAGAAAGCACTAC209390.23601696157045568No Hit
TGAGGTAGTAGTTTGTACAGTT206810.23310887732167695No Hit
TGTCAGTTTGTCAAATACCCCAA194600.21934619953966603No Hit
CAACGGAATCCCAAAAGCAGCT192850.21737366177402156No Hit
TAGCTTATCAGACTGATGTTGACA186470.21018235266270055No Hit
TGAGGTAGTAGTTTGTGCTGTT166140.18726709964809926No Hit
ATCCCGGACGAGCCCCCA165260.18627519494308947No Hit
TGTAAACATCCTACACTCTCAGCT164510.18542982161495614No Hit
ACCACAGGGTAGAACCACGGAC155030.1747443027473506No Hit
TTATAAAGCAATGAGACTGATT152930.17237725742857724No Hit
TCGTTCGCGCTTTCCCCTG151420.17067523912793542No Hit
CCCCCACTGCTAAATTTGACTGGCTT149630.1686576147847905No Hit
CCCATAAAGTAGAAAGCACT146940.16562554244788555No Hit
TACCTGGTTGATCCTGCC142400.16050821590158504No Hit
TGAGGTAGTAGGTTGTGTGGTT139510.15725071067717786No Hit
ACTGGACTTGGAGTCAGAAGGCA139460.15719435245530228No Hit
CGCGACCTCAGATCAGACGTGGCG132450.14929292974834926No Hit
AACGGAATCCCAAAAGCAGCTG131210.14789524584583547No Hit
CTGCGTTCGCGCTTTCCCCTG130060.14659900674269766No Hit
CCAAGTCCTTCTGATCGAGGCCC129950.14647501865457144No Hit
TTCTCACTACTGCACTTGACTAG129560.1460354245239421No Hit
TCGTTTCCCGGCCAATGCACCA123910.13966694545200423No Hit
AGCAGCATTGTACAGGGCTATGT122010.13752533302073305No Hit
GGAATACCGGGTGCTGTAGGCTTT121520.13697302244635262No Hit
CTCGACACAAGGGTTTGT119400.134583433838829No Hit
TAGCAGCACGTAAATATTGGC117580.13253199456255876No Hit
AGCAGCATTGTACAGGGCTATG115790.13051437021941384No Hit
TATTGCACTTGTCCCGGCCTGTT115640.13034529555378715No Hit
CAAGTCCTTCTGATCGAGGCCC113540.12797825023501377No Hit
TGTCAGTTTGTCAAATACCCCA113180.12757247103750977No Hit
ACTGGACTTGGAGTCAGAAGG111520.12570137807124132No Hit
CAAAGTGCTGTTCGTGCAGGTAG109720.12367248208372127No Hit
GGAATACCGGGTGCTGTAGGCTT109280.12317652973121637No Hit
CCCCCCACTGCTAAATTTGACTGG107890.1216097711630759No Hit
TATTGCACTTGTCCCGGCCTGTAG107180.12080948441244299No Hit
CTCCCACTGCTTCACTTGACTAG105960.11943434379867943No Hit
ATAGGCGATAGAAATTGAAACCT102870.11595140568677001No Hit
ATACCGGGTGCTGTAGGCTT102220.11521874880238779No Hit
GGGCTACGCCTGTCTGAGCGTCGCT100680.11348291556862065No Hit
TGAAAAGAACTTTGAAGAGAG100680.11348291556862065No Hit
ACTGGACTTGGAGTCAGAAGGCT99590.1122543063317335No Hit
CTTCTCACTACTGCACTTGACTAG97760.11019159541108814No Hit
CACTAGATTGTGAGCTCCTGGA96410.10866992342044811No Hit
TTCACAGTGGCTAAGTTCCGC96300.1085459353323219No Hit
TTCAAGTAATCCAGGATAGGCTT93290.10515317037541339No Hit
TAGCAGCACGTAAATATTGGCGT93170.10501791064291205No Hit
ATCCCACCGCTGCCACCA92890.10470230460040894No Hit
TTCACAGTGGCTAAGTTCCG92810.10461213144540805No Hit
CCCCCCACTGCTAAATTTGACTGGCT89730.10114046497787375No Hit
ATACCGGGTGCTGTAGGCTTT89590.1009826619566222No Hit
TGTAAACATCCTACACTCAGCT88880.10018237520598931No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)