FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9178133
Sequences flagged as poor quality0
Sequence length18-26
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG7693308.38220583641575No Hit
CCCCCCACTGCTAAATTTGACTG7300977.954744172916213No Hit
CCCCCACTGCTAAATTTGACTG2488552.711390214109994No Hit
TTCAAGTAATCCAGGATAGGCT2175602.3704167285438116No Hit
AACATTCAACGCTGTCGGTGAGT1634351.7806998438571329No Hit
TAGCAGCACGTAAATATTGGCG1343791.4641212978718003No Hit
AAGCTGCCAGTTGAAGAACTGT1321791.4401512813117876No Hit
ACCGGGTGCTGTAGGCTTA1152321.2555058855651797No Hit
AACATTCAACGCTGTCGGTGAG997501.0868223417551262No Hit
ACCGGGTGCTGTAGGCTT905130.9861809585892904No Hit
ACCGGGTGCTGTAGGCTTT779080.8488436591624898No Hit
TCGTACGACTCTTAGCGG660870.720048402000712No Hit
TAGCTTATCAGACTGATGTTGA575180.626685187499462No Hit
TAGCTTATCAGACTGATGTTGAC551920.6013423427182849No Hit
GCCTGTCTGAGCGTCGCT548990.5981499723309741No Hit
ACTGGACTTGGAGTCAGAAGGC490320.534226296350249No Hit
CATAAAGTAGAAAGCACTACT487820.5315024308320657No Hit
CAACGGAATCCCAAAAGCAGCTGT459020.5001235000625944No Hit
TATTGCACTTGTCCCGGCCTGTAA443240.4829304609118216No Hit
TATTGCACTTGTCCCGGCCTGT440370.47980346329694723No Hit
ATCCCGGACGAGCCCCCA439170.4784960078482193No Hit
AGCAGCATTGTACAGGGCTATGA407210.44367411106376425No Hit
AACCGGGCGGAAACACCA396830.4323646214322673No Hit
TGAGGTAGTAGGTTGTATAGTT395280.4306758248109937No Hit
TAGCTTATCAGACTGATGTTGACT382990.41728530192360475No Hit
CACCCGGCTGTGTGCACATGTGC376270.4099635514107281No Hit
CGCCTGTCTGAGCGTCGCT356390.3883033728101347No Hit
TATTGCACTTGTCCCGGCCTGTAT354200.3859172666162061No Hit
CCCATAAAGTAGAAAGCACTAC321910.350735819583351No Hit
TTCAAGTAATTCAGGATAGGTT317660.34610524820243943No Hit
CTCCCACTGCTTCACTTGACTAG306360.333793376060251No Hit
CTACGCCTGTCTGAGCGTCGCT300600.32751758990635677No Hit
CAACACCAGTCGATGGGCTGT298440.3251641700986464No Hit
CCCCCACTGCTAAATTTGACTGGCTT297960.32464118791915525No Hit
CATTGCACTTGTCTCGGTCTGA288140.31394184416373133No Hit
CAACGGAATCCCAAAAGCAGCT279730.3047787605605628No Hit
TCCTGTCTGAGCGTCGCT271660.2959861226678672No Hit
CGCGACCTCAGATCAGACGTGG271380.29568104972983067No Hit
CACTAGATTGTGAGCTCCTGGA253970.2767120502612024No Hit
TATTGCACTTGTCCCGGCCTGTA240320.2618397445319217No Hit
TGAGGTAGTAGATTGTATAGTT232490.2533085977289717No Hit
TTAATGCTAATCGTGATAGGGGTT224770.24489730100882173No Hit
AGCTCGGTCTGAGGCCCCTCAGT223930.24398208219471215No Hit
CCCATAAAGTAGAAAGCACT223800.2438404411877666No Hit
CCCCCCACTGCTAAATTTGACTGG215750.2350695942192165No Hit
CCCCCCACTGCTAAATTTGACTGGCT200720.21869371472389865No Hit
CACTAGATTGTGAGCTCCTGGAA195710.21323508822545933No Hit
CGCGACCTCAGATCAGACGTGGC187410.2041918547050909No Hit
ACCTGGTGCTGTAGGCTTA184660.2011956026350893No Hit
TTCTCACTACTGCACTTGACTAG180440.19659771764039594No Hit
TACGCCTGTCTGAGCGTCGCT166200.181082579648824No Hit
TGTAAACATCCTTGACTGGAAGCT160790.1751881346674754No Hit
TTCACAGTGGCTAAGTTCCG158150.17231173268027386No Hit
CGCGACCTCAGATCAGACGTGGCG154210.168018920623617No Hit
AACGGAATCCCAAAAGCAGCTG151970.16557833711932482No Hit
CTTCTCACTACTGCACTTGACTAG150380.16384595864976026No Hit
AACATTCAACGCTGTCGGTGAGTT146650.1597819512966308No Hit
ACCCCCCACTGCTAAATTTGACTG145630.15867061416521203No Hit
ACCTGGTGCTGTAGGCTT143340.15617555335055616No Hit
CAAAGAATTCTCCTTTTGGGCT142340.15508600714328286No Hit
ATACCGGGTGCTGTAGGCTTA139000.15144692281098998No Hit
ATACCGGGTGCTGTAGGCTT137090.14936588955509797No Hit
TGAGAACTGAATTCCATGGGTT129000.1405514607382569No Hit
ACTGGACTTGGAGTCAGAAGGCA128700.14022459687607491No Hit
ACTGGACTTGGAGTCAGAAGG128650.14017011956571124No Hit
TGAGAACTGAATTCCATAGGCTGT125700.13695595825425497No Hit
ACCTGGTGCTGTAGGCTTT124590.1357465619641816No Hit
TGTAAACATCCCCGACTGGAAGCT124420.13556133910894516No Hit
ACGCCTGTCTGAGCGTCGCT122470.1334367240047622No Hit
TGTAAACATCCTACACTCTCAGCT122120.13305538283221655No Hit
CTGGACGCGAGCCGGGCCCT121110.1319549411628705No Hit
TCGTTTCCCGGGCGGCGCACCA121040.13187867292836136No Hit
ACCACAGGGTAGAACCACGGAC112310.12236693453886535No Hit
TCGTTTCCCGGCCAATGCACCA108110.11779084046831746No Hit
ACTGGACTTGGAGTCAGAAGGCT106910.11648338501958949No Hit
CGCGACCTCAGATCAGACGT106060.11555727074340719No Hit
ACCGGGTGCTGTAGGCTA105840.11531757057780705No Hit
CCCCCCACTGCTAAATTTGACTGT103230.11247385497682372No Hit
TTCAAGTAATCCAGGATAGGCTT102270.11142789061784134No Hit
ACTTGACTTGGAGTCAGAAGGC101450.11053446272787722No Hit
AGCTACATCTGGCTACTGGGTCTC101280.11034923987264075No Hit
ATCCCACCGCTGCCACCA100340.10932506643780385No Hit
ATACCGGGTGCTGTAGGCTTT97870.10663388730583878No Hit
CCCCCACTGCTAAATTTGACTGGCT96480.10511941807772889No Hit
GACCCGCGGGCGCTCTCCAGTCCT96380.10501046345700155No Hit
GGAATACCGGGTGCTGTAGGCTT96380.10501046345700155No Hit
GGAATACCGGGTGCTGTAGGCTTT96160.10477076329140143No Hit
CAAAGAATTCTCCTTTTGGGCTT95110.10362673977376445No Hit
TTCAAGTAATCCAGGATAGGC93860.10226480701467282No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)