FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6955235
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5110767.348076664555547No Hit
CCCCCCACTGCTAAATTTGACTG3359524.830203436691931No Hit
AAGCTGCCAGTTGAAGAACTGT2374423.4138602074552478No Hit
TAGCAGCACGTAAATATTGGCG2165383.1133096149878474No Hit
TTCAAGTAATCCAGGATAGGCT2033222.923294468123651No Hit
AACATTCAACGCTGTCGGTGAGT2017152.9001895694394224No Hit
CCCCCACTGCTAAATTTGACTG1317821.8947167133820786No Hit
TAGCTTATCAGACTGATGTTGAC1183551.7016678803807492No Hit
AACATTCAACGCTGTCGGTGAG991381.4253723993509926No Hit
TAGCTTATCAGACTGATGTTGA851671.2245021196264396No Hit
TAGCTTATCAGACTGATGTTGACT835011.2005489390365676No Hit
TATTGCACTTGTCCCGGCCTGT663920.9545615640593021No Hit
TGAGGTAGTAGGTTGTATAGTT651770.9370927078668082No Hit
TATTGCACTTGTCCCGGCCTGTAA616620.8865552350136264No Hit
TGTAAACATCCTTGACTGGAAGCT534010.7677813905640859No Hit
CATAAAGTAGAAAGCACTACT523850.7531736885957124No Hit
CGCCTGTCTGAGCGTCGCT518320.7452228429377297No Hit
TATTGCACTTGTCCCGGCCTGTAT457730.6581086045259434No Hit
AGCAGCATTGTACAGGGCTATGA434400.6245655251044716No Hit
TGAGGTAGTAGATTGTATAGTT346930.49880413817793356No Hit
TATTGCACTTGTCCCGGCCTGTA339970.4887972872232211No Hit
GCCTGTCTGAGCGTCGCT321440.46215548432224074No Hit
ACTGGACTTGGAGTCAGAAGGC310870.4469582983177419No Hit
CAACGGAATCCCAAAAGCAGCTGT309790.44540551110063137No Hit
TTCAAGTAATTCAGGATAGGTT308260.4432057292097248No Hit
ACCGGGTGCTGTAGGCTT303560.43644822928341026No Hit
CCCATAAAGTAGAAAGCACTAC300890.4326093942188869No Hit
TGTAAACATCCCCGACTGGAAGCT298480.42914437829922353No Hit
CCCCCACTGCTAAATTTGACTGGCTT295150.4243566177131326No Hit
TCGTACGACTCTTAGCGG289300.41594568695378376No Hit
TTCTCACTACTGCACTTGACTAG279010.40115107541298034No Hit
CTACGCCTGTCTGAGCGTCGCT211390.3039293424305577No Hit
CATTGCACTTGTCTCGGTCTGA210130.3021177573439287No Hit
TGATGTAGTAGGTTGTATAGTT202710.29144953405600244No Hit
CTCCCACTGCTTCACTTGACTAG191020.27464205019672233No Hit
ACCGGGTGCTGTAGGCTTT190420.2737793906316609No Hit
AACATTCAACGCTGTCGGTGAGTT189070.2718384066102727No Hit
TGTCAGTTTGTCAAATACCCCAA188210.2706019279003513No Hit
ACCGGGTGCTGTAGGCTTA180710.2598186833370835No Hit
TGTAAACATCCTACACTCTCAGCT175630.25251483235289673No Hit
CACCCGGCTGTGTGCACATGTGC169990.24440583244131936No Hit
CCCATAAAGTAGAAAGCACT166660.2396180718552285No Hit
CCCCCCACTGCTAAATTTGACTGGCT165280.2376339548555872No Hit
TACGCCTGTCTGAGCGTCGCT159390.22916551345856756No Hit
ACGCCTGTCTGAGCGTCGCT157080.22584427413308103No Hit
CAACACCAGTCGATGGGCTGT156300.22472281669850122No Hit
CTTCTCACTACTGCACTTGACTAG154610.22229299225691151No Hit
TGAGGTAGTAGTTTGTACAGTT148660.21373828490338573No Hit
TTCACAGTGGCTAAGTTCCG132460.19044647664672726No Hit
TAGCTTATCAGACTGATGTTGACA131780.1894687958063243No Hit
TGTCAGTTTGTCAAATACCCCA128790.18516987564043486No Hit
CTCCCACTGCTTCACTTGACTAGCCT128060.18412030650294348No Hit
GTCAGGATGGCCGAGCGGTCTAAGG122890.17668705658399753No Hit
TAGCAGCACGTAAATATTGGC121560.1747748278814447No Hit
CAACGGAATCCCAAAAGCAGCT118650.17059092899089678No Hit
TGAGGTAGTAGTTTGTGCTGTT116430.16739908860016953No Hit
CCCCCCACTGCTAAATTTGACTGG110710.1591750674132506No Hit
TGATGTAGTAGATTGTATAGTT108420.1558825834065995No Hit
AACGGAATCCCAAAAGCAGCTG108060.15536498766756263No Hit
ACTTGACTTGGAGTCAGAAGGC105190.15123859941468548No Hit
GCGTTCGCGCTTTCCCCTG104960.15090791324807862No Hit
TTCAAGTAATCCAGGATAGGC100640.14469676437963633No Hit
CAAAGAATTCTCCTTTTGGGCT99630.14324462077844963No Hit
TCCTGTCTGAGCGTCGCT99440.1429714452495135No Hit
TATTGCACTTGTCCCGGCCTGTT97910.14077166335860686No Hit
TGTAAACATCCCCGACTGGAAGC97200.13975084953995084No Hit
AGCTCGGTCTGAGGCCCCTCAGT97000.13946329635159704No Hit
TCGTTTCCCGGCCAATGCACCA96810.1391901208226609No Hit
AACCGGGCGGAAACACCA95670.1375510676490442No Hit
TTCAAGTAATCCAGGATAGGCTT95440.13722038148243734No Hit
TAGCAGCACGTAAATATTGGCGT94450.13579699320008598No Hit
TGAGGTAGTAGGTTGTGTGGTT93210.13401416343229236No Hit
ACCCCCCACTGCTAAATTTGACTG92630.13318025918606632No Hit
TTCACAGTGGCTAAGTTCCGC92400.13284957301945946No Hit
ACCACAGGGTAGAACCACGGAC91770.13194378047614494No Hit
TGTAAACATCCTACACTCAGCT90620.13029034964311054No Hit
TTCACAGTGGCTAAGTTCTGC89930.1292982911432899No Hit
GGGCTACGCCTGTCTGAGCGTCGCT89440.12859378583182307No Hit
AGCAGCATTGTACAGGGCTATG86520.12439550928185748No Hit
CCCCCACTGCTAAATTTGACTGGCT86240.12399293481816215No Hit
ACCTGGTGCTGTAGGCTT84810.12193692952143242No Hit
TTATAAAGCAATGAGACTGATT83770.1204416529419926No Hit
CAAAGTGCTGTTCGTGCAGGT81730.11750861042078378No Hit
CAAAGAATTCTCCTTTTGGGCTT78400.11272084983469285No Hit
TTCACCACCTTCTCCACCCAGC76900.1105642009220393No Hit
CAAAGTGCTGTTCGTGCAGGTAG76760.11036291369019163No Hit
AGCAGCATTGTACAGGGCTATGT75280.10823502009637345No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC74310.10684038713285747No Hit
ATCACATTGCCAGGGATTTCCA72680.10449682864777393No Hit
ACTGGACTTGGAGTCAGAAGG71980.10349039248853563No Hit
TCCCACTGCTTCACTTGACTAG71750.10315970632192874No Hit
ATCCCACCGCTGCCACCA71060.1021676478221081No Hit
CACTAGATTGTGAGCTCCTGGA70790.10177945101783045No Hit
ACTGGACTTGGAGTCAGAAGGCA69620.10009726486596068No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)