FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10129298
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG196259119.375390081326465No Hit
CAACGGAATCCCAAAAGCAGCTG103137110.182058026133696No Hit
CCCCCACTGCTAAATTTGACTG6001335.924724497196153No Hit
TTCAAGTAATCCAGGATAGGCT3242073.2006857730910867No Hit
TAGCAGCACGTAAATATTGGCG2658172.6242391131152427No Hit
AAGCTGCCAGTTGAAGAACTGT2208832.1806348278034666No Hit
AACATTCAACGCTGTCGGTGAGT2118162.091122208074044No Hit
AACATTCAACGCTGTCGGTGAG1529841.510311968312118No Hit
TAGCTTATCAGACTGATGTTGAC1004420.9915988255059729No Hit
TATTGCACTTGTCCCGGCCTGTAA918690.9069631478904066No Hit
TATTGCACTTGTCCCGGCCTGTAT865630.8545804457525091No Hit
TAGCTTATCAGACTGATGTTGACT859930.8489532048519057No Hit
TAGCTTATCAGACTGATGTTGA763760.7540107912710239No Hit
CATAAAGTAGAAAGCACTACT677410.6687630278031114No Hit
AGCAGCATTGTACAGGGCTATGA658730.6503214734130638No Hit
CAACGGAATCCCAAAAGCAGCTGT634160.626065103425726No Hit
TATTGCACTTGTCCCGGCCTGT627900.6198850107875196No Hit
CCCATAAAGTAGAAAGCACTAC545250.5382900177287706No Hit
TGAGGTAGTAGGTTGTATAGTT516650.5100550897011817No Hit
TATTGCACTTGTCCCGGCCTGTA484200.47801930597757114No Hit
CTCCCACTGCTTCACTTGACTAG479860.4737347050111469No Hit
TGTAAACATCCTTGACTGGAAGCT459850.45398012774429186No Hit
CAACGGAATCCCAAAAGCAGCT458800.452943530736286No Hit
ACTGGACTTGGAGTCAGAAGGC452330.44655611869647827No Hit
CCCATAAAGTAGAAAGCACT434200.42865754369157666No Hit
TTCAAGTAATTCAGGATAGGTT426930.42148034345519303No Hit
CCCCCACTGCTAAATTTGACTGGCTT399320.39422277832086683No Hit
CTTCTCACTACTGCACTTGACTAG397570.392495116640857No Hit
CCCCCCACTGCTAAATTTGACTGG387220.38227723184765616No Hit
TCGTACGACTCTTAGCGG366900.362216611654628No Hit
CCCCCCACTGCTAAATTTGACTGGCT365260.3605975458516474No Hit
TGAGGTAGTAGATTGTATAGTT349120.3446635689857283No Hit
CATTGCACTTGTCTCGGTCTGA348700.34424893018252595No Hit
TTCTCACTACTGCACTTGACTAG343660.33927326454409773No Hit
TGTAAACATCCCCGACTGGAAGCT321940.3178305150070617No Hit
CACCCGGCTGTGTGCACATGTGC301920.2980660653877495No Hit
ACCCCCCACTGCTAAATTTGACTG293970.29021754518427634No Hit
TGTAAACATCCTACACTCTCAGCT275140.2716279055073708No Hit
CAAAGAATTCTCCTTTTGGGCT264670.2612915524846835No Hit
CCCCCCACTGCTAAATTTGACTGA256120.25285069113377845No Hit
CAACACCAGTCGATGGGCTGT249330.24614736381534044No Hit
CACTAGATTGTGAGCTCCTGGA246420.24327450925029553No Hit
CACTAGATTGTGAGCTCCTGGAA241230.2381507583250093No Hit
CCCCCCACTGCTAAATTTGACTGT225690.2228091226065222No Hit
AACGGAATCCCAAAAGCAGCTG205470.202847225938066No Hit
TTCACAGTGGCTAAGTTCCG198780.1962426221441999No Hit
CCCATAAAGTAGAAAGCACTA194920.19243189409572115No Hit
CGCCTGTCTGAGCGTCGCT185910.18353690453178492No Hit
ACCGGGTGCTGTAGGCTTA179030.17674472604123206No Hit
AGCTCGGTCTGAGGCCCCTCAGT175000.17276616800098094No Hit
ATCCCGGACGAGCCCCCA172220.17002165401787964No Hit
CCCCCCACTGCTAAATTTGACTGTA169480.16731662944460712No Hit
TCGTTTCCCGGGCGGCGCACCA163040.16095883446217102No Hit
TAGCAGCACGTAAATATTGGC160610.15855985281507168No Hit
CTCCCACTGCTTCACTTGACTAGCCT158120.15610163705322916No Hit
AACATTCAACGCTGTCGGTGAGTT157750.1557363600123128No Hit
TGTCAGTTTGTCAAATACCCCAA153640.15167882315240405No Hit
ACTGGACTTGGAGTCAGAAGGCA147990.14610094401408666No Hit
TTCAAGTAATCCAGGATAGGC147470.14558758168631233No Hit
TCGTTTCCCGGCCAATGCACCA144450.14260613124423824No Hit
CTACGCCTGTCTGAGCGTCGCT142850.14102655485108642No Hit
TTCAAGTAATCCAGGATAGGCTT141400.13959506374479258No Hit
ACTGGACTTGGAGTCAGAAGGCT141130.1393285102284482No Hit
AGCAGCATTGTACAGGGCTATGT135520.1337901204999596No Hit
CTCGTACGACTCTTAGCGG135440.13371114168030201No Hit
TGTCAGTTTGTCAAATACCCCA132550.13085803182017153No Hit
TATTGCACTTGTCCCGGCCTGTT131650.12996952009902363No Hit
CAAAGAATTCTCCTTTTGGGCTT129970.12831096488621424No Hit
ACCGGGTGCTGTAGGCTT123320.12174585050217694No Hit
ACCACAGGGTAGAACCACGGAC122150.12059078526468468No Hit
TAGCTTATCAGACTGATGTTGACA121460.11990959294513796No Hit
CCCCCACTGCTAAATTTGACTGGCT121210.11966278413370798No Hit
CAACGGAATCCCAAAAGCAGCTGA120270.11873478300273128No Hit
CCCCCCACTGCTAAATTTGACTGGC119080.11755997306032462No Hit
AGCAGCATTGTACAGGGCTATG117370.1158718007901436No Hit
ACTGGACTTGGAGTCAGAAGG116790.11529920434762606No Hit
ACCGGGTGCTGTAGGCTTT116390.11490431024933812No Hit
TGAGGTAGTAGTTTGTACAGTT116140.11465750143790814No Hit
TATTGCACTTGTCCCGGCCTGTAG112470.11103434808611615No Hit
GGAATACCGGGTGCTGTAGGCTTT109600.10820098293090005No Hit
GACCCGCGGGCGCTCTCCAGTCCT109370.10797391882438447No Hit
TTCACAGTGGCTAAGTTCTG109330.10793442941455568No Hit
GCCTGTCTGAGCGTCGCT107920.10654242771809064No Hit
TGTAAACATCCTACACTCAGCT106330.10497272367739602No Hit
TGTAAACATCCCCGACTGGAAGC103020.10170497501406316No Hit
CCCCCACTGCTAAATTTGACTGG102970.10165561325177717No Hit
CCCCCCACTGCTAAATTTGAC101680.1003820797847985No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)