FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8211401
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG110173913.417186665223147No Hit
CCCCCCACTGCTAAATTTGACTG97991411.93357869138287No Hit
CCCCCACTGCTAAATTTGACTG5284526.435588762502283No Hit
GCCTGTCTGAGCGTCGCT2878863.505930352201774No Hit
TTCAAGTAATCCAGGATAGGCT2742733.3401486542917587No Hit
TCGTACGACTCTTAGCGG2206002.6865086725152016No Hit
TAGCAGCACGTAAATATTGGCG2057452.5056016628587496No Hit
AACATTCAACGCTGTCGGTGAG1421411.7310200780597613No Hit
TAGCTTATCAGACTGATGTTGAC1300831.584175465307321No Hit
AAGCTGCCAGTTGAAGAACTGT1283971.5636430372843804No Hit
TGAGGTAGTAGATTGTATAGTT1074831.30894837555735No Hit
TAGCTTATCAGACTGATGTTGA1000141.2179894758519283No Hit
TGAGGTAGTAGGTTGTATAGTT843931.027753972799526No Hit
TATTGCACTTGTCCCGGCCTGT818150.9963585994643303No Hit
TAGCTTATCAGACTGATGTTGACT689010.8390894562328645No Hit
CATAAAGTAGAAAGCACTACT567580.691209697346409No Hit
AGCAGCATTGTACAGGGCTATGA458870.5588205959007483No Hit
TATTGCACTTGTCCCGGCCTGTAA456800.5562997106096754No Hit
CAACGGAATCCCAAAAGCAGCTGT455510.5547287241239345No Hit
AACATTCAACGCTGTCGGTGAGT454470.5534621923834921No Hit
TTCTCACTACTGCACTTGACTAG446620.5439023133811149No Hit
TATTGCACTTGTCCCGGCCTGTA422610.5146624796426335No Hit
TGTAAACATCCTTGACTGGAAGCT412820.5027400318167387No Hit
TATTGCACTTGTCCCGGCCTGTAT407600.4963830167349031No Hit
CATTGCACTTGTCTCGGTCTGA393990.4798085004008451No Hit
TTCAAGTAATTCAGGATAGGTT380840.4637941807981366No Hit
CCCATAAAGTAGAAAGCACT380760.46369675527964105No Hit
CAACGGAATCCCAAAAGCAGCT365690.445344223233039No Hit
TGAGGTAGTAGTTTGTACAGTT359070.43728226157753103No Hit
TGAGAACTGAATTCCATGGGTT356580.4342498923143566No Hit
CTCCCACTGCTTCACTTGACTAG324520.39520661577725896No Hit
CCCCCACTGCTAAATTTGACTGGCTT280710.34185396621112524No Hit
AACGGAATCCCAAAAGCAGCTG268500.3269843964507396No Hit
CAACACCAGTCGATGGGCTGT259180.31563432354600635No Hit
CCCATAAAGTAGAAAGCACTAC255380.311006611417467No Hit
TCCTGTCTGAGCGTCGCT251960.3068416705017816No Hit
AACCGGGCGGAAACACCA250690.3052950403956645No Hit
CTTCTCACTACTGCACTTGACTAG246010.29959564756367396No Hit
CACCCGGCTGTGTGCACATGTGC241880.29456605517134044No Hit
CCCCCCACTGCTAAATTTGACTGG224040.27284016454682947No Hit
TGTAAACATCCCCGACTGGAAGCT217000.26426671891921977No Hit
ACTGGACTTGGAGTCAGAAGGC211740.25786099107813637No Hit
ACCGGGTGCTGTAGGCTT196600.23942321170285072No Hit
CCCATAAAGTAGAAAGCACTA196560.23937449894360294No Hit
TAGCAGCACGTAAATATTGGC194890.23734074124500804No Hit
TCGTTTCCCGGCCAATGCACCA176900.21543217777331786No Hit
TAGCTTATCAGACTGATGTTGACA172940.21060961460778738No Hit
TGAGAACTGAATTCCATAGGCTGT169340.20622546627548696No Hit
GGAATACCGGGTGCTGTAGGCTTT166490.2027546821790825No Hit
ACCCCCCACTGCTAAATTTGACTG164880.2007939936193592No Hit
TGTCAGTTTGTCAAATACCCCA164400.20020944050838582No Hit
TTCACAGTGGCTAAGTTCTG152370.18555907816461528No Hit
ACCGGGTGCTGTAGGCTTT152220.1853764053174361No Hit
TGAGGTAGTAGATTGTATAGT146130.17795988772196122No Hit
GGAATACCGGGTGCTGTAGGCTT145680.17741186918042365No Hit
CAAAGAATTCTCCTTTTGGGCT137850.16787634655767025No Hit
TTAATGCTAATCGTGATAGGGGTT136300.16598872713681867No Hit
TGTAAACATCCTACACTCTCAGCT131760.16045982896219535No Hit
TATTGCACTTGTCCCGGCCTGTT126890.15452905052377786No Hit
TTCAAGTAATCCAGGATAGGC122230.1488540140714112No Hit
TGATGTAGTAGATTGTATAGTT121610.14809896630307057No Hit
CCCCCCACTGCTAAATTTGACTGGCT120010.14615045593315928No Hit
TTCACAGTGGCTAAGTTCCG119790.14588253575729648No Hit
TGGCTCAGTTCAGCAGGAAC116680.14209511872578137No Hit
TTCACAGTGGCTAAGTTCCGC116420.1417784857906708No Hit
CACTAGATTGTGAGCTCCTGGA116280.14160799113330358No Hit
TGTAAACATCCCCGACTGGA113780.13856344368031714No Hit
CTCCCACTGCTTCACTTGACTAGCCT112150.13657839874097003No Hit
TGAGGTAGTAGTTTGTGCTGTT110970.13514137234316045No Hit
TCCCACTGCTTCACTTGACTAG106980.13028227460819414No Hit
ACCGGGTGCTGTAGGCTTA102880.12528921678529645No Hit
AGCAGCATTGTACAGGGCTATG102430.12474119824375889No Hit
TTCACAGTGGCTAAGTTCTGC101640.12377912124861519No Hit
TGATGTAGTAGGTTGTATAGTT98010.11935843834687894No Hit
CAAAGTGCTGTTCGTGCAGGTAG94760.1154005266579966No Hit
TCGTTTCCCGGCCAACGCACCA92670.11285528498729998No Hit
TGTCAGTTTGTCAAATACCCCAA91670.1116374660061054No Hit
TGAGGTAGTAAGTTGTATTGTT90020.10962806468713439No Hit
AGCAGCATTGTACAGGGCTATGT89970.10956717373807466No Hit
CACTAGATTGTGAGCTCCTGGAA89170.108592918553119No Hit
CCCCCACTGCTAAATTTGACTGG87530.10659569542395993No Hit
AGCTCGGTCTGAGGCCCCTCAGT86730.1056214402390043No Hit
CTCGTACGACTCTTAGCGG86460.10529262911408174No Hit
AATGCACCCGGGCAAGGATTCT85430.10403827556345135No Hit
ACTGGACTTGGAGTCAGAAGGCT84450.10284481296188068No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)