FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8962624
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG183089920.428158092987054No Hit
CCCCCACTGCTAAATTTGACTG5772686.440836969173313No Hit
CAACGGAATCCCAAAAGCAGCTG5608296.257419702087246No Hit
TAGCAGCACGTAAATATTGGCG2359082.6321309473654146No Hit
AAGCTGCCAGTTGAAGAACTGT2111082.355426268021508No Hit
TTCAAGTAATCCAGGATAGGCT2104772.348385919123685No Hit
TAGCTTATCAGACTGATGTTGAC1918482.140533843660071No Hit
AACATTCAACGCTGTCGGTGAGT1600581.7858386115494749No Hit
TAGCTTATCAGACTGATGTTGACT1236341.37943977120986No Hit
AACATTCAACGCTGTCGGTGAG1213561.3540231075185123No Hit
TATTGCACTTGTCCCGGCCTGTAA932811.0407777900757635No Hit
TGTAAACATCCTTGACTGGAAGCT918681.0250123178212094No Hit
TATTGCACTTGTCCCGGCCTGTAT786010.8769864718189673No Hit
AGCAGCATTGTACAGGGCTATGA782280.8728247441820609No Hit
TAGCTTATCAGACTGATGTTGA715060.797824387143765No Hit
TGAGGTAGTAGGTTGTATAGTT665910.742985536378632No Hit
TATTGCACTTGTCCCGGCCTGTA502120.5602377160974287No Hit
TATTGCACTTGTCCCGGCCTGT496920.5544358437886048No Hit
CGCCTGTCTGAGCGTCGCT473070.5278253332952492No Hit
TGTAAACATCCCCGACTGGAAGCT465930.5198589163173643No Hit
TGAGGTAGTAGATTGTATAGTT425850.47513986975242967No Hit
ACTGGACTTGGAGTCAGAAGGC407400.45455438050285274No Hit
CAACGGAATCCCAAAAGCAGCTGT332510.37099626180904166No Hit
CATAAAGTAGAAAGCACTACT331940.37036028734442056No Hit
TTCAAGTAATTCAGGATAGGTT323340.36076488314136573No Hit
CCCCCACTGCTAAATTTGACTGGCTT310000.34588084917988304No Hit
CCCCCCACTGCTAAATTTGACTGG297480.33191172585171486No Hit
TGTCAGTTTGTCAAATACCCCAA290620.3242577173827665No Hit
TGTAAACATCCTACACTCTCAGCT269060.300202262194643No Hit
CAACGGAATCCCAAAAGCAGCT263320.2937978877614413No Hit
TAGCTTATCAGACTGATGTTGACA261170.2913990367106776No Hit
CCCATAAAGTAGAAAGCACTAC253380.2827073856941896No Hit
TACGCCTGTCTGAGCGTCGCT244500.27279957298219804No Hit
TTAATGCTAATCGTGATAGGGGTT228540.25499228797280793No Hit
CATTGCACTTGTCTCGGTCTGA217950.24317655186695325No Hit
CCCCCCACTGCTAAATTTGACTGGCT217210.24235090080762062No Hit
TTCTCACTACTGCACTTGACTAG214960.23984047528937952No Hit
CTTCTCACTACTGCACTTGACTAG213480.23818917317071428No Hit
ACCCCCCACTGCTAAATTTGACTG210250.23458531787119485No Hit
CTACGCCTGTCTGAGCGTCGCT208190.2322868838411608No Hit
GCGTTCGCGCTTTCCCCTG186790.2084099478010011No Hit
TGTCAGTTTGTCAAATACCCCA183220.20442673931205863No Hit
TAGCAGCACGTAAATATTGGC176500.19692893509757858No Hit
GGAATACCGGGTGCTGTAGGCTTT176430.19685083297034442No Hit
CACCCGGCTGTGTGCACATGTGC169460.1890740925871709No Hit
AACCGGGCGGAAACACCA168530.18803645003963126No Hit
CCCATAAAGTAGAAAGCACT167630.1870322798323348No Hit
AACATTCAACGCTGTCGGTGAGTT166660.18595000749780422No Hit
CAAAGAATTCTCCTTTTGGGCT161500.18019276497597134No Hit
GCCTGTCTGAGCGTCGCT161230.17989151391378239No Hit
TCGTTTCCCGGCCAATGCACCA160030.1785526203040538No Hit
TGAGGTAGTAGTTTGTACAGTT158830.17721372669432522No Hit
CTCCCACTGCTTCACTTGACTAG156610.17473677351632738No Hit
CCCCCCACTGCTAAATTTGACTGTA155190.17315241607814855No Hit
AGCAGCATTGTACAGGGCTATGT150850.1683100841896302No Hit
ACTGGACTTGGAGTCAGAAGGCA144260.16095732678287072No Hit
AACGGAATCCCAAAAGCAGCTG140050.15626004170207297No Hit
CCCCCCACTGCTAAATTTGACTGT139720.1558918459593976No Hit
CAACACCAGTCGATGGGCTGT137040.15290165023100377No Hit
TGAGGTAGTAGTTTGTGCTGTT129510.14450009282995696No Hit
GGAATACCGGGTGCTGTAGGCTT128410.14327277368770575No Hit
CCCCCCACTGCTAAATTTGACTGA124720.13915567583779037No Hit
TTCACAGTGGCTAAGTTCCG123110.13735932691140454No Hit
AGCAGCATTGTACAGGGCTATG121380.13542908862404582No Hit
TAGCAGCACGTAAATATTGGCGT120890.13488237373340664No Hit
GGGCTACGCCTGTCTGAGCGTCGCT117270.13084337801072543No Hit
TGTAAACATCCTACACTCAGCT115510.1288796673831235No Hit
TATTGCACTTGTCCCGGCCTGTT112030.12499687591491064No Hit
ACTGGACTTGGAGTCAGAAGGCT110630.1234348333702273No Hit
TTCAAGTAATCCAGGATAGGCTT110590.12339020358323634No Hit
TATTGCACTTGTCCCGGCCTGTAG108940.12154922486985954No Hit
CACTAGATTGTGAGCTCCTGGAA107920.12041116530159025No Hit
ACTGGACTTGGAGTCAGAAGG107270.11968593126298727No Hit
ACGCCTGTCTGAGCGTCGCT106300.11860365892845666No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT105620.11784495254961047No Hit
TGTAAACATCCCCGACTGGAAGC103560.11554651851957641No Hit
TCGTACGACTCTTAGCGG101950.11375016959319056No Hit
CCAAGTCCTTCTGATCGAGGCCC101830.1136162802322177No Hit
CAAAGAATTCTCCTTTTGGGCTT101570.11332618661677651No Hit
CTCCCACTGCTTCACTTGACTAGCCT99770.11131784620218364No Hit
CAACGGAATCCCAAAAGCAGCTGA97030.1082607057933034No Hit
CACTAGATTGTGAGCTCCTGGA95480.10653130154740398No Hit
TCGTTTCCCGGGCGGCGCACCA94220.10512546325718895No Hit
TTATAAAGCAATGAGACTGATT92560.10327332709706442No Hit
AGCTCGGTCTGAGGCCCCTCAGT92100.10276008454666848No Hit
ACCACAGGGTAGAACCACGGAC91870.1025034632714705No Hit
ATCACATTGCCAGGGATTTCCA90530.10100836540727358No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)