FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9884513
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG158947416.08044827297005No Hit
CAACGGAATCCCAAAAGCAGCTG7349047.434903469700531No Hit
CCCCCACTGCTAAATTTGACTG5503605.567902030175893No Hit
TTCAAGTAATCCAGGATAGGCT3083563.119587176424372No Hit
TAGCAGCACGTAAATATTGGCG2832722.865816454487945No Hit
AACATTCAACGCTGTCGGTGAGT2652322.683308727501294No Hit
AAGCTGCCAGTTGAAGAACTGT2453542.4822062553815245No Hit
AACATTCAACGCTGTCGGTGAG1668811.6883077598259015No Hit
TAGCTTATCAGACTGATGTTGAC1334681.3502739082846065No Hit
TAGCTTATCAGACTGATGTTGACT1190151.2040552731328291No Hit
TATTGCACTTGTCCCGGCCTGTAA980720.9921783703456104No Hit
TAGCTTATCAGACTGATGTTGA889210.8995992012960072No Hit
TGTAAACATCCTTGACTGGAAGCT833180.8429145674652864No Hit
TGAGGTAGTAGGTTGTATAGTT822370.8319782674169178No Hit
TATTGCACTTGTCCCGGCCTGTAT813420.8229236989217373No Hit
AGCAGCATTGTACAGGGCTATGA651160.6587679129968265No Hit
TATTGCACTTGTCCCGGCCTGT648640.6562184702473455No Hit
CATAAAGTAGAAAGCACTACT574160.5808682734293535No Hit
TATTGCACTTGTCCCGGCCTGTA546180.5525613654410693No Hit
TGTAAACATCCCCGACTGGAAGCT485480.4911521690547627No Hit
CAACGGAATCCCAAAAGCAGCTGT485150.4908183134566165No Hit
ACTGGACTTGGAGTCAGAAGGC434380.43945513552362164No Hit
CGCCTGTCTGAGCGTCGCT427680.4326768551976208No Hit
TGAGGTAGTAGATTGTATAGTT425100.430066711430295No Hit
CCCCCACTGCTAAATTTGACTGGCTT417700.42258025256277165No Hit
CCCATAAAGTAGAAAGCACTAC390450.3950118736249323No Hit
TTCAAGTAATTCAGGATAGGTT385730.39023672688780925No Hit
TTCTCACTACTGCACTTGACTAG378540.38296272158274264No Hit
CCCCCCACTGCTAAATTTGACTGG351050.35515153857352405No Hit
TTAATGCTAATCGTGATAGGGGTT317250.3209566318542957No Hit
CCCCCCACTGCTAAATTTGACTGGCT307230.3108195618742168No Hit
CTTCTCACTACTGCACTTGACTAG295730.29918520012063315No Hit
CCCATAAAGTAGAAAGCACT285110.2884411199621064No Hit
TGTAAACATCCTACACTCTCAGCT266920.27003859471882935No Hit
CTCCCACTGCTTCACTTGACTAG257270.2602758476821266No Hit
TGTCAGTTTGTCAAATACCCCAA249010.2519193408921613No Hit
GCCTGTCTGAGCGTCGCT247280.25016912821097No Hit
ACCCCCCACTGCTAAATTTGACTG246270.24914732774391615No Hit
CATTGCACTTGTCTCGGTCTGA243080.24592005696183517No Hit
CTACGCCTGTCTGAGCGTCGCT240540.24335038053973929No Hit
CAAAGAATTCTCCTTTTGGGCT235280.2380289246420132No Hit
AACATTCAACGCTGTCGGTGAGTT226650.22929809490867176No Hit
CAACGGAATCCCAAAAGCAGCT205390.20778970091900328No Hit
TACGCCTGTCTGAGCGTCGCT197100.19940284362011565No Hit
TGAGGTAGTAGTTTGTACAGTT195650.19793590235553335No Hit
AACCGGGCGGAAACACCA193930.19619580651064955No Hit
TAGCTTATCAGACTGATGTTGACA189460.19167358068121312No Hit
CACCCGGCTGTGTGCACATGTGC182950.18508752024505407No Hit
TCGTACGACTCTTAGCGG174890.17693335018123807No Hit
TTCACAGTGGCTAAGTTCCG173410.1754360584077334No Hit
CAACACCAGTCGATGGGCTGT169600.17158154377458962No Hit
CCCCCCACTGCTAAATTTGACTGTA165740.1676764449599085No Hit
TAGCAGCACGTAAATATTGGC164880.16680639703746658No Hit
AACGGAATCCCAAAAGCAGCTG163730.16564296086210822No Hit
TGTCAGTTTGTCAAATACCCCA162650.16455034254090212No Hit
ACCGGGTGCTGTAGGCTT155090.15690201429245934No Hit
CTCCCACTGCTTCACTTGACTAGCCT151690.15346228994792155No Hit
TATTGCACTTGTCCCGGCCTGTT147660.14938520491601356No Hit
GGAATACCGGGTGCTGTAGGCTTT147590.14931438706186131No Hit
CCCCCCACTGCTAAATTTGACTGA145670.1473719544908282No Hit
AGCTCGGTCTGAGGCCCCTCAGT144880.14657272442253858No Hit
ACTGGACTTGGAGTCAGAAGGCA143260.1449337969407294No Hit
CCCCCCACTGCTAAATTTGACTGT141930.1435882577118367No Hit
ACCGGGTGCTGTAGGCTTA139410.14103881496235576No Hit
TGAGGTAGTAGTTTGTGCTGTT138230.13984502827807502No Hit
GACCCGCGGGCGCTCTCCAGTCCT135580.13716406665659706No Hit
TTCAAGTAATCCAGGATAGGC131260.1327935933717726No Hit
ACCGGGTGCTGTAGGCTTT130760.13228775155639938No Hit
ACTGGACTTGGAGTCAGAAGGCT127250.1287367420124795No Hit
TGAGGTAGTAGGTTGTGTGGTT125280.12674372525990912No Hit
CACTAGATTGTGAGCTCCTGGAA124410.12586356050115974No Hit
CCCCCACTGCTAAATTTGACTGGCT124160.12561063959347316No Hit
CACTAGATTGTGAGCTCCTGGA123640.125084564105485No Hit
TGTAAACATCCCCGACTGGAAGC123590.12503397992394769No Hit
AGCAGCATTGTACAGGGCTATGT122710.12414369832889087No Hit
AGCAGCATTGTACAGGGCTATG122340.12376937538551469No Hit
TTCAAGTAATCCAGGATAGGCTT120290.12169542394248457No Hit
ACGCCTGTCTGAGCGTCGCT119860.12126039998126362No Hit
TATTGCACTTGTCCCGGCCTGTAG117650.11902457915731407No Hit
ACTGGACTTGGAGTCAGAAGG117230.11859967203240059No Hit
CAAAGAATTCTCCTTTTGGGCTT116130.11748682003857953No Hit
CTCCCCACTGCTAAATTTGACTG116110.1174665863659646No Hit
GGAATACCGGGTGCTGTAGGCTT112240.11355137071497605No Hit
CCCATAAAGTAGAAAGCACTA108840.11011164637043828No Hit
TTCACAGTGGCTAAGTTCTG103910.10512404607085851No Hit
ACCACAGGGTAGAACCACGGAC103150.10435516651149125No Hit
CGCCCCACTGCTAAATTTGACTG101510.10269600535706717No Hit
GCGTTCGCGCTTTCCCCTG100020.10118859674725503No Hit
TCGTTTCCCGGCCAATGCACCA99760.10092555900326095No Hit
CCCCCACTGCTAAATTTGACTGG99630.10079404013126393No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)