FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7715537
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG97011712.573551264156988No Hit
CAACGGAATCCCAAAAGCAGCTG5172146.703538587139171No Hit
CCCCCACTGCTAAATTTGACTG3635804.712309719984494No Hit
TTCAAGTAATCCAGGATAGGCT2757653.5741517408315193No Hit
TAGCAGCACGTAAATATTGGCG2450753.176382927072996No Hit
AAGCTGCCAGTTGAAGAACTGT2379433.083946068821911No Hit
AACATTCAACGCTGTCGGTGAGT1833442.3762960374631086No Hit
GCCTGTCTGAGCGTCGCT1379541.78800257195319No Hit
AACATTCAACGCTGTCGGTGAG1152861.4942057824361417No Hit
TAGCTTATCAGACTGATGTTGAC1120201.4518756115096072No Hit
TAGCTTATCAGACTGATGTTGACT860871.1157616119266875No Hit
TAGCTTATCAGACTGATGTTGA761260.986658478858957No Hit
TGTAAACATCCTTGACTGGAAGCT638120.8270584406503396No Hit
TGAGGTAGTAGGTTGTATAGTT625630.8108703256818028No Hit
CGCCTGTCTGAGCGTCGCT621340.8053101164572214No Hit
TATTGCACTTGTCCCGGCCTGTAA542720.7034118299218837No Hit
AGCAGCATTGTACAGGGCTATGA528680.685214781550526No Hit
CATAAAGTAGAAAGCACTACT527550.6837502042955662No Hit
TATTGCACTTGTCCCGGCCTGT525590.6812098756055476No Hit
TATTGCACTTGTCCCGGCCTGTAT465740.6036391245353369No Hit
TTCTCACTACTGCACTTGACTAG346160.4486531527228759No Hit
TGTAAACATCCCCGACTGGAAGCT342640.44409092976937314No Hit
CAACGGAATCCCAAAAGCAGCTGT331550.4297173352937067No Hit
TATTGCACTTGTCCCGGCCTGTA331240.4293155486131426No Hit
CCCATAAAGTAGAAAGCACTAC326160.4227314313961556No Hit
TGAGGTAGTAGATTGTATAGTT319290.41382732012042717No Hit
TTCAAGTAATTCAGGATAGGTT306000.39660233629882147No Hit
CCCCCACTGCTAAATTTGACTGGCTT298680.3871149862932418No Hit
CCCCCCACTGCTAAATTTGACTGG294610.3818399160032542No Hit
ACTGGACTTGGAGTCAGAAGGC291560.3778868535009294No Hit
CTACGCCTGTCTGAGCGTCGCT281610.364990797140886No Hit
CTCCCACTGCTTCACTTGACTAG263800.3419075042994415No Hit
CTTCTCACTACTGCACTTGACTAG262980.34084471372504593No Hit
TGTAAACATCCTACACTCTCAGCT256320.3322127805232481No Hit
TCCACCACGTTCCCGTGG253920.3291021739640416No Hit
TTCACAGTGGCTAAGTTCCG243220.31523405305424623No Hit
CCCATAAAGTAGAAAGCACT229640.2976332042734032No Hit
CCCCCCACTGCTAAATTTGACTGGCT227660.29506695386205783No Hit
TACGCCTGTCTGAGCGTCGCT226290.29329131595117747No Hit
CATTGCACTTGTCTCGGTCTGA224440.29089355672845585No Hit
TGTCAGTTTGTCAAATACCCCAA197570.25606772412600703No Hit
CAACACCAGTCGATGGGCTGT189190.24520652289011122No Hit
TCGTTTCCCGGCCAATGCACCA187140.24254954645412238No Hit
ACGCCTGTCTGAGCGTCGCT178710.23162354091490978No Hit
ACCCCCCACTGCTAAATTTGACTG176920.22930354685616827No Hit
GCGTTCGCGCTTTCCCCTG170710.2212548523842216No Hit
CAAAGAATTCTCCTTTTGGGCT164100.21268772348574053No Hit
AACATTCAACGCTGTCGGTGAGTT159180.20631098003936732No Hit
TGAGGTAGTAGTTTGTACAGTT154460.2001934538062613No Hit
TAGCAGCACATCATGGTTTACA152140.19718653413236176No Hit
TAGCAGCACGTAAATATTGGC149950.19434810564708588No Hit
TGTCAGTTTGTCAAATACCCCA148400.19233917224426503No Hit
GACCCGCGGGCGCTCTCCAGTCCT144990.18791951875805923No Hit
TAGCTTATCAGACTGATGTTGACA144070.1867271195770301No Hit
TGTAAACATCCTACACTCAGCT139920.1813483624017356No Hit
CACCCGGCTGTGTGCACATGTGC137310.17796557776859862No Hit
CAACGGAATCCCAAAAGCAGCT135370.17545117079990674No Hit
AACCGGGCGGAAACACCA130780.16950213575542442No Hit
AGCTCGGTCTGAGGCCCCTCAGT129690.16808940194311817No Hit
TTCAAGTAATCCAGGATAGGC128520.16657298124550501No Hit
GTCAGGATGGCCGAGCGGTCTAAGG124990.16199779743133888No Hit
TAGCAGCACGTAAATATTGGCGT122390.15862797365886522No Hit
GGAATACCGGGTGCTGTAGGCTTT121700.15773367427309337No Hit
GGGCTACGCCTGTCTGAGCGTCGCT120780.15654127509206422No Hit
AACGGAATCCCAAAAGCAGCTG120310.1559321146408863No Hit
TGAGGTAGTAGTTTGTGCTGTT119460.1548304414845007No Hit
AGCAGCATTGTACAGGGCTATG117290.15201793472055153No Hit
ATCCCACCGCTGCCACCA110960.14381370992064452No Hit
ACCGGGTGCTGTAGGCTT110010.1425824281576253No Hit
TATTGCACTTGTCCCGGCCTGTT105490.1367241191377865No Hit
TCAGGCTCAGTCCCCTCCCGAT105450.13667227569513307No Hit
GGAATACCGGGTGCTGTAGGCTT105050.13615384126859867No Hit
TTCACAGTGGCTAAGTTCCGC105030.13612791954727196No Hit
TGAGGTAGTAGGTTGTGTGGTT104940.1360112718013017No Hit
CCCCCCACTGCTAAATTTGACTGA102750.13317284331602583No Hit
AGCAGCATTGTACAGGGCTATGT99470.12892168101844367No Hit
TTCACAGTGGCTAAGTTCTG99060.12839028573124592No Hit
TCGTACGACTCTTAGCGG98370.12749598634547407No Hit
ATCACATTGCCAGGGATTTCC96770.12542224863933646No Hit
CTCCCACTGCTTCACTTGACTAGCCT96050.12448906667157451No Hit
GCTTGACTAGCTTGCTGTTT95430.12368549331044619No Hit
TGTAAACATCCCCGACTGGAAGC93730.12148214699767496No Hit
CCCCCCACTGCTAAATTTGACTGTA93550.12124885150573447No Hit
TGATGTAGTAGGTTGTATAGTT92100.11936952670954726No Hit
TTCAAGTAATCCAGGATAGGCTT91870.11907142691428996No Hit
TCCCACTGCTTCACTTGACTAG89690.11624595928967744No Hit
CCCCCACTGCTAAATTTGACTGGCT89440.11592193777309344No Hit
ACTGGACTTGGAGTCAGAAGG88690.11494987322334142No Hit
ACTGGACTTGGAGTCAGAAGGCA87430.11331680477975803No Hit
TCGTTTCCCGGGCGGCGCACCA85760.11115234104897688No Hit
TTCACAGTGGCTAAGTTCTGC85050.11023211994187831No Hit
ACCACAGGGTAGAACCACGGAC84630.10968776379401718No Hit
CAAAGTGCTGTTCGTGCAGGT83980.10884530785089878No Hit
ATCCCGGACGAGCCCCCA82880.10741961317792915No Hit
ACTGGACTTGGAGTCAGAAGGCT79410.10292219452774318No Hit
CCCATAAAGTAGAAAGCACTA79380.10288331194575309No Hit
CCCCCCACTGCTAAATTTGACTGT77740.10075773079696203No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)