FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9891168
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG218438722.08421694990925No Hit
CCCCCACTGCTAAATTTGACTG6924407.000588808116494No Hit
CAACGGAATCCCAAAAGCAGCTG5408515.4680195503705935No Hit
AAGCTGCCAGTTGAAGAACTGT2551162.5792302789721093No Hit
TAGCAGCACGTAAATATTGGCG2282052.307159275830721No Hit
AACATTCAACGCTGTCGGTGAGT1896951.9178220408348137No Hit
TAGCTTATCAGACTGATGTTGAC1863331.8838321217474012No Hit
TTCAAGTAATCCAGGATAGGCT1778311.7978766511700135No Hit
AACATTCAACGCTGTCGGTGAG1333461.3481319900743776No Hit
TAGCTTATCAGACTGATGTTGACT1171331.1842180822325532No Hit
TAGCTTATCAGACTGATGTTGA885640.8953846502253324No Hit
TGTAAACATCCTTGACTGGAAGCT827080.8361803176328619No Hit
TATTGCACTTGTCCCGGCCTGTAA817410.8264039191327051No Hit
CGCCTGTCTGAGCGTCGCT810970.8198930601522489No Hit
TGAGGTAGTAGGTTGTATAGTT688750.6963282799362017No Hit
AGCAGCATTGTACAGGGCTATGA668450.6758049201065032No Hit
TATTGCACTTGTCCCGGCCTGTAT644110.6511971083698104No Hit
TATTGCACTTGTCCCGGCCTGT589960.5964512987748262No Hit
TATTGCACTTGTCCCGGCCTGTA499820.5053194931073863No Hit
CATAAAGTAGAAAGCACTACT406860.41133665912862866No Hit
TGAGGTAGTAGATTGTATAGTT399630.4040271078198247No Hit
TGTAAACATCCCCGACTGGAAGCT386040.3902875777663467No Hit
ACTGGACTTGGAGTCAGAAGGC345650.34945316872587745No Hit
TACGCCTGTCTGAGCGTCGCT340300.34404430295795196No Hit
CCCCCCACTGCTAAATTTGACTGG326140.3297285012245268No Hit
CTACGCCTGTCTGAGCGTCGCT301010.3043219971594861No Hit
TTCAAGTAATTCAGGATAGGTT295560.298812031096833No Hit
CATTGCACTTGTCTCGGTCTGA279990.283070715207749No Hit
ACCCCCCACTGCTAAATTTGACTG263290.26618696598824326No Hit
CTCCCACTGCTTCACTTGACTAG261110.263982979563182No Hit
TTCTCACTACTGCACTTGACTAG250240.2529933775262942No Hit
CAACGGAATCCCAAAAGCAGCTGT242990.24566360615854466No Hit
TAGCTTATCAGACTGATGTTGACA231930.23448191356167442No Hit
TGTCAGTTTGTCAAATACCCCAA229110.2316308852503567No Hit
TGTAAACATCCTACACTCTCAGCT225340.22781940413912696No Hit
CCCATAAAGTAGAAAGCACTAC224200.22666686077923254No Hit
CTTCTCACTACTGCACTTGACTAG222840.22529189677093744No Hit
GCCTGTCTGAGCGTCGCT217660.22005490150404888No Hit
GCGTTCGCGCTTTCCCCTG211440.21376646317199344No Hit
CAACACCAGTCGATGGGCTGT203360.20559755935800503No Hit
ACGCCTGTCTGAGCGTCGCT202930.20516282809067646No Hit
AACATTCAACGCTGTCGGTGAGTT188420.1904931753257047No Hit
CACCCGGCTGTGTGCACATGTGC187210.189269861759501No Hit
CAACGGAATCCCAAAAGCAGCT183130.18514496973461575No Hit
CCCCCACTGCTAAATTTGACTGGCTT179780.18175810986124188No Hit
ACCGGGTGCTGTAGGCTT175900.1778354184258118No Hit
CCCATAAAGTAGAAAGCACT173560.17546967152918644No Hit
AACCGGGCGGAAACACCA170380.17225468215684941No Hit
CTCCCCACTGCTAAATTTGACTG163130.16492491078909993No Hit
TGAGGTAGTAGTTTGTACAGTT161660.16343873645660453No Hit
TAGCAGCACGTAAATATTGGC160370.16213454265461874No Hit
CCCCCCACTGCTAAATTTGACTGTA154230.1559269845583454No Hit
TCGTACGACTCTTAGCGG147840.14946667572525307No Hit
CGCCCCACTGCTAAATTTGACTG143480.14505870287513062No Hit
CCCCCCACTGCTAAATTTGACTGGCT142970.14454309137201998No Hit
TGTCAGTTTGTCAAATACCCCA141830.14339054801212556No Hit
AACGGAATCCCAAAAGCAGCTG134780.13626297723383124No Hit
TGAGGTAGTAGTTTGTGCTGTT132770.13423086130980688No Hit
TAGCAGCACGTAAATATTGGCGT126530.12792220291880596No Hit
CCTCGACACAAGGGTTTGT124300.12566766634638094No Hit
GGAATACCGGGTGCTGTAGGCTTT122080.12342323980342867No Hit
CAAAGAATTCTCCTTTTGGGCT121760.12309971886030042No Hit
ACCGGGTGCTGTAGGCTTT121140.12247289703298944No Hit
AGCAGCATTGTACAGGGCTATGT120090.12141134393834985No Hit
CCCCCCACTGCTAAATTTGACTGT119020.12032957078476475No Hit
CCCCCCACTGCTAAATTTGACTGA118010.11930845780801619No Hit
TTATAAAGCAATGAGACTGATT117870.11916691739539759No Hit
GGGCTACGCCTGTCTGAGCGTCGCT117770.11906581710067002No Hit
CTCCCACTGCTTCACTTGACTA114600.11586093775780575No Hit
AGCAGCATTGTACAGGGCTATG111870.11310089971174284No Hit
CTCCCACTGCTTCACTTGACTAGCCT111810.11304023953490629No Hit
ACTGGACTTGGAGTCAGAAGGCA111800.11303012950543354No Hit
TGTAAACATCCTACACTCAGCT110650.11186747611606636No Hit
ACCGGGTGCTGTAGGCTTA110520.11173604573292054No Hit
CCAAGTCCTTCTGATCGAGGCCC109940.11114966402350057No Hit
TTCACAGTGGCTAAGTTCCG107200.1083795159479649No Hit
CCCGCCACTGCTAAATTTGACTG106330.10749994338383495No Hit
GGAATACCGGGTGCTGTAGGCTT105230.10638784014183157No Hit
CGCGACCTCAGATCAGACGTGGCG103700.10484100563249961No Hit
TATTGCACTTGTCCCGGCCTGTT103620.10476012539671756No Hit
TGTAAACATCCCCGACTGGAAGC102710.10384011271469659No Hit
CAAAGTGCTGTTCGTGCAGGTAG99430.10052402304763199No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)