FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7022150
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG113237616.12577344545474No Hit
CAACGGAATCCCAAAAGCAGCTG107612915.324779447889892No Hit
CCCCCACTGCTAAATTTGACTG5649998.045954586558247No Hit
TCGTACGACTCTTAGCGG2825154.023198023397392No Hit
TTCAAGTAATCCAGGATAGGCT1588412.261999530058458No Hit
TAGCAGCACGTAAATATTGGCG1357651.933382226241251No Hit
AACATTCAACGCTGTCGGTGAG1139721.623035679955569No Hit
AAGCTGCCAGTTGAAGAACTGT1042491.4845738128635817No Hit
TATTGCACTTGTCCCGGCCTGT760701.083286457851228No Hit
TGAGGTAGTAGATTGTATAGTT741001.0552323718519256No Hit
TAGCTTATCAGACTGATGTTGAC685480.9761682675533847No Hit
TGAGGTAGTAGGTTGTATAGTT600210.8547382212000598No Hit
TAGCTTATCAGACTGATGTTGA597910.8514628710580093No Hit
GCCTGTCTGAGCGTCGCT582580.829631950328603No Hit
AACATTCAACGCTGTCGGTGAGT388640.5534487300897872No Hit
TATTGCACTTGTCCCGGCCTGTAA372830.5309342580263879No Hit
TAGCTTATCAGACTGATGTTGACT366360.521720555670272No Hit
CAACGGAATCCCAAAAGCAGCT351520.5005874269276503No Hit
TATTGCACTTGTCCCGGCCTGTA343630.48935155187513796No Hit
CAACGGAATCCCAAAAGCAGCTGT341110.4857629073716739No Hit
TGAGAACTGAATTCCATGGGTT328410.46767727832643846No Hit
CATTGCACTTGTCTCGGTCTGA315070.44868024750254554No Hit
TATTGCACTTGTCCCGGCCTGTAT302520.43080822824918297No Hit
CATAAAGTAGAAAGCACTACT299680.4267638828563901No Hit
CTCCCACTGCTTCACTTGACTAG296530.42227807722706007No Hit
AACGGAATCCCAAAAGCAGCTG287650.40963237754818677No Hit
AGCAGCATTGTACAGGGCTATGA279260.3976844698560982No Hit
ACCGGGTGCTGTAGGCTT275750.3926860007262733No Hit
TTCTCACTACTGCACTTGACTAG273770.3898663514735516No Hit
TGAGGTAGTAGTTTGTACAGTT250880.35726949723375323No Hit
CCCATAAAGTAGAAAGCACT249290.35500523343990087No Hit
CACCCGGCTGTGTGCACATGTGC242700.3456206432502866No Hit
TGTAAACATCCTTGACTGGAAGCT207060.2948669567012952No Hit
CCCCCCACTGCTAAATTTGACTGG199960.28475609321931317No Hit
TTCAAGTAATTCAGGATAGGTT198800.2831041774954964No Hit
TCGTTTCCCGGCCAATGCACCA186480.2655596932563389No Hit
CAACACCAGTCGATGGGCTGT184390.2625833968229104No Hit
ACCCCCCACTGCTAAATTTGACTG181290.25816879445753793No Hit
ACCGGGTGCTGTAGGCTTT167020.23784738292403326No Hit
CCCATAAAGTAGAAAGCACTAC166690.23737744138191294No Hit
ACTGGACTTGGAGTCAGAAGGC156910.2234500829518025No Hit
CCCCCACTGCTAAATTTGACTGGCTT154350.21980447583717236No Hit
CTTCTCACTACTGCACTTGACTAG141870.20203214115335047No Hit
TGAGAACTGAATTCCATAGGCTGT135790.1933738242561039No Hit
TCGTATGACTCTTAGCGG131090.18668071744408762No Hit
TGTAAACATCCCCGACTGGAAGCT129020.18373290231624217No Hit
TAGCAGCACGTAAATATTGGC124480.1772676459488903No Hit
CCCATAAAGTAGAAAGCACTA117290.16702861659178456No Hit
TTCACAGTGGCTAAGTTCCGC113930.16224375725383253No Hit
TCCCACTGCTTCACTTGACTAG111500.1587832786254922No Hit
CACTAGATTGTGAGCTCCTGGA108280.1541977884266215No Hit
CTCCCACTGCTTCACTTGACTA106250.15130693590994213No Hit
CTCGTACGACTCTTAGCGG103640.14759012553135434No Hit
TGAGGTAGTAGATTGTATAGT95610.13615488133976061No Hit
ACCGGGTGCTGTAGGCTTA94120.13403302407382353No Hit
GGAATACCGGGTGCTGTAGGCTT91820.13075767393177304No Hit
TCGTTTCCCGGCCAACGCACCA91340.13007412259777987No Hit
GGAATACCGGGTGCTGTAGGCTTT90320.12862157601304444No Hit
CTCCCACTGCTTCACTTGACTAGCCT88990.12672756919177175No Hit
AATGCACCCGGGCAAGGATTCT88060.12540318848216No Hit
TAGCTTATCAGACTGATGTTGACA86510.12319588729947381No Hit
CCCCCCACTGCTAAATTTGACTGTA86000.12246961400710608No Hit
TATTGCACTTGTCCCGGCCTGTT84720.12064681044979102No Hit
TGAGGTAGTAGTTTGTGCTGTT84340.12010566564371311No Hit
CCCCCCACTGCTAAATTTGACTGA83980.11959300214321825No Hit
CCCCCACTGCTAAATTTGACTGG83380.1187385629757268No Hit
TGGCTCAGTTCAGCAGGAAC81170.11559137870880001No Hit
TGTCAGTTTGTCAAATACCCCA80850.11513567781947125No Hit
TGATGTAGTAGATTGTATAGTT80140.11412459147127305No Hit
TGTAAACATCCCCGACTGGA78030.1111198137322615No Hit
AACCGGGCGGAAACACCA76930.10955334192519386No Hit
TGTAAACATCCTACACTCTCAGCT73630.10485392650399095No Hit
CCCCCCACTGCTAAATTTGACTGGCT72780.10364347101671141No Hit
CAAAGTGCTGTTCGTGCAGGTAG71500.10182066745939634No Hit
CACTAGATTGTGAGCTCCTGGAA71000.10110863481982015No Hit
CCCCCCACTGCTAAATTTGACTGT70530.1004393241386185No Hit
TTCAAGTAATCCAGGATAGGC70270.10006906716603887No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)