FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10627823
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG238862022.475157894518944No Hit
CAACGGAATCCCAAAAGCAGCTG8600558.092485168411253No Hit
CCCCCACTGCTAAATTTGACTG7155306.73261118481179No Hit
AAGCTGCCAGTTGAAGAACTGT2514492.3659502044774365No Hit
TAGCAGCACGTAAATATTGGCG2418602.27572476508124No Hit
AACATTCAACGCTGTCGGTGAGT2361372.221875543090998No Hit
TTCAAGTAATCCAGGATAGGCT2218002.086974914806165No Hit
AACATTCAACGCTGTCGGTGAG1595021.5007965413048374No Hit
TATTGCACTTGTCCCGGCCTGTAA1448671.363091952133565No Hit
TATTGCACTTGTCCCGGCCTGTAT1283311.2075003507303423No Hit
TAGCTTATCAGACTGATGTTGACT1191721.121320895163572No Hit
TAGCTTATCAGACTGATGTTGAC1185871.115816475302609No Hit
TAGCTTATCAGACTGATGTTGA864120.8130733829496408No Hit
TATTGCACTTGTCCCGGCCTGT806570.7589230644883718No Hit
TATTGCACTTGTCCCGGCCTGTA716280.6739668133351486No Hit
TGTAAACATCCTTGACTGGAAGCT611940.5757905452508948No Hit
TGAGGTAGTAGGTTGTATAGTT601580.5660425470013943No Hit
AGCAGCATTGTACAGGGCTATGA567490.5339663635722951No Hit
CATAAAGTAGAAAGCACTACT501600.4719687183348838No Hit
CCCATAAAGTAGAAAGCACTAC448040.4215726964967331No Hit
TGTAAACATCCCCGACTGGAAGCT439750.41377241604418896No Hit
CAACGGAATCCCAAAAGCAGCTGT433980.4083432703009826No Hit
TGAGGTAGTAGATTGTATAGTT410430.3861844518863365No Hit
ACCCCCCACTGCTAAATTTGACTG389720.3666978646520553No Hit
ACTGGACTTGGAGTCAGAAGGC375590.3534025736032676No Hit
CCCCCCACTGCTAAATTTGACTGG366000.344379088737176No Hit
CTCCCACTGCTTCACTTGACTAG348100.32753650488910097No Hit
CAACGGAATCCCAAAAGCAGCT317970.2991863902889613No Hit
TTCTCACTACTGCACTTGACTAG312100.29366315189855907No Hit
CATTGCACTTGTCTCGGTCTGA305230.28719898703619734No Hit
TTCAAGTAATTCAGGATAGGTT301560.283745786884106No Hit
CACCCGGCTGTGTGCACATGTGC279490.2629795396479599No Hit
CCCATAAAGTAGAAAGCACT277900.2614834665575443No Hit
CCCCCACTGCTAAATTTGACTGGCTT266900.25113327536598984No Hit
CTTCTCACTACTGCACTTGACTAG260890.24547830726951322No Hit
CCCCCCACTGCTAAATTTGACTGT258630.24335181344288478No Hit
CCCCCCACTGCTAAATTTGACTGA255480.2403878950562124No Hit
TGTAAACATCCTACACTCTCAGCT248650.23396136725272898No Hit
CAAAGAATTCTCCTTTTGGGCT246920.23233356445623907No Hit
CACTAGATTGTGAGCTCCTGGA233730.2199227442910933No Hit
CCCCCCACTGCTAAATTTGACTGTA230050.21646013487428237No Hit
CACTAGATTGTGAGCTCCTGGAA226760.2133644867815356No Hit
TCGTACGACTCTTAGCGG220390.20737078515515361No Hit
GGAATACCGGGTGCTGTAGGCTTT211780.19926940823158232No Hit
TTAATGCTAATCGTGATAGGGGTT203310.19129976101408538No Hit
CCCCCCACTGCTAAATTTGACTGGCT188280.17715763614053415No Hit
AACGGAATCCCAAAAGCAGCTG186470.17545455922628744No Hit
GACCCGCGGGCGCTCTCCAGTCCT180680.17000659495364198No Hit
TATTGCACTTGTCCCGGCCTGTAG178510.16796478450948987No Hit
TAGCTTATCAGACTGATGTTGACA175490.16512318656417216No Hit
AACATTCAACGCTGTCGGTGAGTT175420.16505732171113502No Hit
TCGTTTCCCGGGCGGCGCACCA166420.15658898346349953No Hit
TCGTTTCCCGGCCAATGCACCA165330.1555633736090637No Hit
TATTGCACTTGTCCCGGCCTGTT163940.15425548581304No Hit
GGAATACCGGGTGCTGTAGGCTT163140.1535027446354724No Hit
AGCTCGGTCTGAGGCCCCTCAGT160040.15058587257239794No Hit
AACCGGGCGGAAACACCA157090.14781013948011743No Hit
TGAGGTAGTAGTTTGTACAGTT154280.14516613609391124No Hit
CAACACCAGTCGATGGGCTGT153480.14441339491634364No Hit
TGTCAGTTTGTCAAATACCCCAA149560.1407249631462624No Hit
CAAAGAATTCTCCTTTTGGGCTT149030.14022627211612387No Hit
ACCGGGTGCTGTAGGCTTA147280.13857965079019477No Hit
CTCCCACTGCTTCACTTGACTAGCCT137980.12982903460097142No Hit
TAGCAGCACGTAAATATTGGC134380.12644169930191726No Hit
TGTAAACATCCCCGACTGGAAGC132540.12471039459351176No Hit
ACTGGACTTGGAGTCAGAAGGCA130340.12264035635520087No Hit
TGAGGTAGTAGTTTGTGCTGTT120250.11314640825312954No Hit
TATTGCACTTGTCCCGGCCTGTTT119740.1126665357524302No Hit
CCCATAAAGTAGAAAGCACTA118110.11113282560313623No Hit
CCCCCCACTGCTAAATTTGAC115730.10889342059987263No Hit
ACCGGGTGCTGTAGGCTT115120.10831945545197733No Hit
TGTCAGTTTGTCAAATACCCCA114890.10810304236342663No Hit
CTCCCACTGCTTCACTTGACTA111740.10513912397675422No Hit
TTCAAGTAATCCAGGATAGGCTT109390.10292794676764941No Hit
TCAGTGCACTACAGAACTTTGT108850.10241984647279126No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)