FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14805369
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG283478719.14702024650652No Hit
CAACGGAATCCCAAAAGCAGCTG10072946.803572406739744No Hit
CCCCCACTGCTAAATTTGACTG9506096.420704543061372No Hit
TTCAAGTAATCCAGGATAGGCT3638902.4578245905252345No Hit
AAGCTGCCAGTTGAAGAACTGT3631782.4530155243006777No Hit
TAGCAGCACGTAAATATTGGCG3543922.3936721874341664No Hit
AACATTCAACGCTGTCGGTGAGT3433602.319158678179517No Hit
AACATTCAACGCTGTCGGTGAG2008481.3565889509407025No Hit
TAGCTTATCAGACTGATGTTGAC1938051.3090183702952625No Hit
TAGCTTATCAGACTGATGTTGACT1611931.0887469268749734No Hit
TAGCTTATCAGACTGATGTTGA1438480.9715934807163537No Hit
TATTGCACTTGTCCCGGCCTGTAA1238140.8362777043922378No Hit
TGAGGTAGTAGGTTGTATAGTT1170310.7904632434355402No Hit
TATTGCACTTGTCCCGGCCTGTAT996340.6729585733391718No Hit
TATTGCACTTGTCCCGGCCTGT951500.6426722630148562No Hit
TGTAAACATCCTTGACTGGAAGCT927800.6266645566213176No Hit
TATTGCACTTGTCCCGGCCTGTA800830.54090512705222No Hit
AGCAGCATTGTACAGGGCTATGA776830.5246947914638264No Hit
CATAAAGTAGAAAGCACTACT739230.4992985990420097No Hit
TGAGGTAGTAGATTGTATAGTT648630.43810458219582366No Hit
ACTGGACTTGGAGTCAGAAGGC604230.4081154613572955No Hit
TTCTCACTACTGCACTTGACTAG561990.37958527072172266No Hit
TTAATGCTAATCGTGATAGGGGTT555160.3749720793855256No Hit
CCCATAAAGTAGAAAGCACTAC539140.36415168038027285No Hit
CAACGGAATCCCAAAAGCAGCTGT534740.3611797855224007No Hit
TGTAAACATCCCCGACTGGAAGCT522480.3528990057593296No Hit
CCCCCCACTGCTAAATTTGACTGG490830.3315216257021355No Hit
TTCAAGTAATTCAGGATAGGTT474350.3203905285981052No Hit
ACCCCCCACTGCTAAATTTGACTG444110.2999655057567292No Hit
CTCCCACTGCTTCACTTGACTAG402380.27177978475240977No Hit
CATTGCACTTGTCTCGGTCTGA400480.2704964665183286No Hit
CCCCCACTGCTAAATTTGACTGGCTT384120.25944642109224025No Hit
AACCGGGCGGAAACACCA375590.25368499765186536No Hit
CTTCTCACTACTGCACTTGACTAG375330.2535093856829911No Hit
ACCGGGTGCTGTAGGCTT374160.2527191318230569No Hit
CGCCTGTCTGAGCGTCGCT355850.2403519966304116No Hit
CCCATAAAGTAGAAAGCACT333540.22528313883970066No Hit
TGTAAACATCCTACACTCTCAGCT329960.22286509711443195No Hit
CAAAGAATTCTCCTTTTGGGCT304610.20574293014919118No Hit
GGAATACCGGGTGCTGTAGGCTTT296670.2003800107920309No Hit
AACATTCAACGCTGTCGGTGAGTT294020.19859011957081246No Hit
CCCCCCACTGCTAAATTTGACTGTA292980.1978876716953154No Hit
TAGCTTATCAGACTGATGTTGACA279150.18854646581250356No Hit
CAACGGAATCCCAAAAGCAGCT275560.18612166978073966No Hit
TGAGGTAGTAGTTTGTACAGTT274870.18565562263257337No Hit
TCGTACGACTCTTAGCGG272130.18380494265289843No Hit
CCCCCCACTGCTAAATTTGACTGGCT262650.17740186009548292No Hit
AACGGAATCCCAAAAGCAGCTG256790.17344383648931683No Hit
CAACACCAGTCGATGGGCTGT247940.16746627524109667No Hit
ACCGGGTGCTGTAGGCTTT247750.16733794341768854No Hit
GACCCGCGGGCGCTCTCCAGTCCT241710.16325834229460948No Hit
TTCACAGTGGCTAAGTTCCG239240.16159002859030397No Hit
GGAATACCGGGTGCTGTAGGCTT238590.1611509986681183No Hit
ACCGGGTGCTGTAGGCTTA237700.1605498653900487No Hit
CTACGCCTGTCTGAGCGTCGCT237580.16046881371210672No Hit
CCCCCCACTGCTAAATTTGACTGA228890.1545993213678092No Hit
AGCTCGGTCTGAGGCCCCTCAGT217110.146642748316506No Hit
CACCCGGCTGTGTGCACATGTGC213980.14452865038351964No Hit
CCCCCCACTGCTAAATTTGACTGT212030.14321156061696266No Hit
TGAGGTAGTAGTTTGTGCTGTT208560.14086781626314074No Hit
CGCCCCACTGCTAAATTTGACTG207870.14040176911497446No Hit
TACGCCTGTCTGAGCGTCGCT205560.13884152431459157No Hit
GCCTGTCTGAGCGTCGCT200910.13570077179434029No Hit
CACTAGATTGTGAGCTCCTGGA197640.13349211357042165No Hit
CTCCCACTGCTTCACTTGACTA196590.13278291138842943No Hit
CTCCCACTGCTTCACTTGACTAGCCT196580.13277615708193424No Hit
ACTGGACTTGGAGTCAGAAGGCA195880.1323033556272728No Hit
TAGCAGCACGTAAATATTGGC193660.13080389958534638No Hit
CGCGACCTCAGATCAGACGTGGCG177710.12003078072555977No Hit
TATTGCACTTGTCCCGGCCTGTT177390.11981464291771451No Hit
TGTCAGTTTGTCAAATACCCCAA174300.11772756221070882No Hit
CACTAGATTGTGAGCTCCTGGAA173620.11726826936903768No Hit
TGAGGTAGTAGGTTGTGTGGTT162570.10980476069188144No Hit
CAAAGAATTCTCCTTTTGGGCTT159120.10747452495104987No Hit
TGTAAACATCCCCGACTGGAAGC158210.10685988305998992No Hit
TTCAAGTAATCCAGGATAGGC157880.1066369909456495No Hit
TGTCAGTTTGTCAAATACCCCA150860.10189546778604436No Hit
GCCCCCACTGCTAAATTTGACTG150440.1016117869132475No Hit
ACTGGACTTGGAGTCAGAAGG149830.10119977421704249No Hit
ACTGGACTTGGAGTCAGAAGGCT149050.10067293831041968No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)