FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8245233
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG168529420.439616442615993No Hit
CAACGGAATCCCAAAAGCAGCTG5586246.7751147845063935No Hit
CCCCCACTGCTAAATTTGACTG5123156.21346904326415No Hit
AAGCTGCCAGTTGAAGAACTGT2309512.8010245435150223No Hit
TAGCAGCACGTAAATATTGGCG2292412.780285287268413No Hit
TTCAAGTAATCCAGGATAGGCT2057152.494956782907166No Hit
AACATTCAACGCTGTCGGTGAGT1823492.2115687937502795No Hit
TAGCTTATCAGACTGATGTTGAC1522681.846739807110363No Hit
TAGCTTATCAGACTGATGTTGACT1310621.5895487732123519No Hit
AACATTCAACGCTGTCGGTGAG1083501.3140926399532917No Hit
TAGCTTATCAGACTGATGTTGA956951.160610015508355No Hit
TATTGCACTTGTCCCGGCCTGTAA838791.0173029676662866No Hit
TGTAAACATCCTTGACTGGAAGCT820610.9952538636567335No Hit
TGAGGTAGTAGGTTGTATAGTT656310.7959872086089016No Hit
TATTGCACTTGTCCCGGCCTGTAT648330.7863088890271507No Hit
AGCAGCATTGTACAGGGCTATGA546600.6629285066898656No Hit
TATTGCACTTGTCCCGGCCTGT451340.5473950827102156No Hit
TATTGCACTTGTCCCGGCCTGTA425730.5163347112204106No Hit
TGTAAACATCCCCGACTGGAAGCT411960.49963415224287777No Hit
TGAGGTAGTAGATTGTATAGTT382090.46340715902146123No Hit
ACCCCCCACTGCTAAATTTGACTG381080.4621822087987083No Hit
TTCTCACTACTGCACTTGACTAG379330.46005977029393835No Hit
CATAAAGTAGAAAGCACTACT374630.45435950688112753No Hit
CCCCCCACTGCTAAATTTGACTGG357500.4335838659744364No Hit
TTCAAGTAATTCAGGATAGGTT320340.3885154003531495No Hit
CCCCCACTGCTAAATTTGACTGGCTT319020.3869144753095516No Hit
CAACGGAATCCCAAAAGCAGCTGT288080.34938976254521853No Hit
CTTCTCACTACTGCACTTGACTAG283010.3432407549913993No Hit
CCCATAAAGTAGAAAGCACTAC280140.33975995584357654No Hit
TAGCTTATCAGACTGATGTTGACA275760.334447795471638No Hit
CTCCCACTGCTTCACTTGACTAG250840.3042242711637136No Hit
CAAAGAATTCTCCTTTTGGGCT249610.302732500100361No Hit
TGTAAACATCCTACACTCTCAGCT246790.29931234205267454No Hit
ACTGGACTTGGAGTCAGAAGGC241850.29332100135920963No Hit
TGTCAGTTTGTCAAATACCCCAA228730.2774087766834485No Hit
GACCCGCGGGCGCTCTCCAGTCCT205290.24898022893955818No Hit
CCCCCCACTGCTAAATTTGACTGTA203760.24712461127538785No Hit
CATTGCACTTGTCTCGGTCTGA191490.23224328530194355No Hit
CCCCCCACTGCTAAATTTGACTGGCT173250.21012141197222686No Hit
AACATTCAACGCTGTCGGTGAGTT171000.20739256246609405No Hit
CGCCTGTCTGAGCGTCGCT168440.20428773813911627No Hit
GCCTGTCTGAGCGTCGCT166320.20171655549333778No Hit
TGAGGTAGTAGTTTGTACAGTT152150.18453086771471466No Hit
TGATGTAGTAGGTTGTATAGTT151070.18322101995177092No Hit
TGTCAGTTTGTCAAATACCCCA150570.18261460895040807No Hit
TAGCAGCACGTAAATATTGGC145100.1759804725954985No Hit
TTCACAGTGGCTAAGTTCCG141220.17127472322492282No Hit
GTGCGGTAACGCGACCGATCCCGGA140860.17083810730394156No Hit
CTCCCACTGCTTCACTTGACTAGCCT138080.16746646213636412No Hit
CCCCCCACTGCTAAATTTGACTGA136700.16579276777260266No Hit
CAAAGAATTCTCCTTTTGGGCTT132180.16031081232028252No Hit
CCCATAAAGTAGAAAGCACT130760.15858860507641204No Hit
GGAATACCGGGTGCTGTAGGCTTT130620.15841880999603045No Hit
CCCCCCACTGCTAAATTTGACTGT130510.1582853995757306No Hit
TGTAAACATCCCCGACTGGAAGC130350.15809134805529448No Hit
GGGCTACGCCTGTCTGAGCGTCGCT124270.15071739027872227No Hit
CAACGGAATCCCAAAAGCAGCT116350.14111184001713475No Hit
CACCCGGCTGTGTGCACATGTGC114970.13943814565337329No Hit
TGAGGTAGTAGTTTGTGCTGTT111880.13569052566495088No Hit
TAGCAGCACGTAAATATTGGCGT109830.13320424055936322No Hit
CAACACCAGTCGATGGGCTGT109420.13270698353824567No Hit
CTACGCCTGTCTGAGCGTCGCT107380.13023282665268526No Hit
ATCACATTGCCAGGGATTTCCA106230.12883808134955071No Hit
AACGGAATCCCAAAAGCAGCTG105300.1277101568870158No Hit
GGAATACCGGGTGCTGTAGGCTT104730.12701884834546215No Hit
TGTAAACATCCTACACTCAGCT96940.11757096494422899No Hit
CTCCCACTGCTTCACTTGACTA95450.1157638601601677No Hit
TATTGCACTTGTCCCGGCCTGTT95120.11536362889926823No Hit
AGCAGCATTGTACAGGGCTATGT93470.11336247259477082No Hit
TAGCAGCACATCATGGTTTACA93000.11279244625348975No Hit
TCGTTTCCCGGCCAATGCACCA91850.1113977009503552No Hit
CAAAGTGCTGTTCGTGCAGGTAG91000.11036680224803835No Hit
TTCAAGTAATCCAGGATAGGCTT90320.1095420832861849No Hit
ACTGGACTTGGAGTCAGAAGGCA89150.10812308154299582No Hit
TATTGCACTTGTCCCGGCCTGTAG89010.10795328646261422No Hit
CACTAGATTGTGAGCTCCTGGAA87470.10608554057841664No Hit
CCCCCACTGCTAAATTTGACTGG86960.10546700135702652No Hit
TGATGTAGTAGATTGTATAGTT86220.10456951307500953No Hit
AGCTCGGTCTGAGGCCCCTCAGT85910.10419353825416457No Hit
AGCAGCATTGTACAGGGCTATG85440.10362351191288348No Hit
TTCAAGTAATCCAGGATAGGC83570.10135553476778643No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)