FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9664900
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG9004019.316195718527869No Hit
CCCCCCACTGCTAAATTTGACTG7260917.512659210131507No Hit
AACATTCAACGCTGTCGGTGAGT3567803.691502240064563No Hit
CCCCCACTGCTAAATTTGACTG3395003.5127109437241977No Hit
TTCAAGTAATCCAGGATAGGCT2611522.7020662396920816No Hit
AACATTCAACGCTGTCGGTGAG2521492.6089147326925266No Hit
TAGCAGCACGTAAATATTGGCG2427142.5112934432844627No Hit
AAGCTGCCAGTTGAAGAACTGT2165272.2403439249242103No Hit
TATTGCACTTGTCCCGGCCTGTAA2026082.096327949590787No Hit
TAGCTTATCAGACTGATGTTGAC1823611.8868379393475359No Hit
TATTGCACTTGTCCCGGCCTGTAT1658361.7158584155035232No Hit
TGAGGTAGTAGGTTGTATAGTT1651741.7090088878312242No Hit
TAGCTTATCAGACTGATGTTGACT1482601.5340044904758456No Hit
TAGCTTATCAGACTGATGTTGA1143161.182795476414655No Hit
TATTGCACTTGTCCCGGCCTGT994651.0291363594036151No Hit
TGAGGTAGTAGATTGTATAGTT992151.0265496797690612No Hit
GCCTGTCTGAGCGTCGCT956840.9900154166106219No Hit
TATTGCACTTGTCCCGGCCTGTA849280.878726112013575No Hit
CATAAAGTAGAAAGCACTACT761620.7880267773075769No Hit
AGCAGCATTGTACAGGGCTATGA671910.6952063653012447No Hit
TGTAAACATCCTTGACTGGAAGCT662720.6856977309646245No Hit
TTCAAGTAATTCAGGATAGGTT647530.6699810655050751No Hit
CATTGCACTTGTCTCGGTCTGA610400.6315636995726805No Hit
TGTAAACATCCCCGACTGGAAGCT567060.586721021428054No Hit
CACCCGGCTGTGTGCACATGTGC552060.5712009436207307No Hit
ACTGGACTTGGAGTCAGAAGGC550410.5694937350619251No Hit
CAACGGAATCCCAAAAGCAGCT496350.5135593746443315No Hit
CAACGGAATCCCAAAAGCAGCTGT463090.4791461887862264No Hit
TCCTGTCTGAGCGTCGCT396520.41026808347732513No Hit
GGAATACCGGGTGCTGTAGGCTTT379870.3930407971111962No Hit
CAACACCAGTCGATGGGCTGT357860.37026766960858365No Hit
TGAGGTAGTAGTTTGTACAGTT352310.364525240819874No Hit
TGATGTAGTAGGTTGTATAGTT350920.36308704694306204No Hit
CCCATAAAGTAGAAAGCACTAC290260.30032385229024616No Hit
CCCATAAAGTAGAAAGCACT276980.28658341007149585No Hit
CACTAGATTGTGAGCTCCTGGA274890.2844209458970087No Hit
GGAATACCGGGTGCTGTAGGCTT259000.2679800101397842No Hit
AACATTCAACGCTGTCGGTGAGTT237750.245993233246076No Hit
TTCTCACTACTGCACTTGACTAG236380.24457573280634048No Hit
AACGGAATCCCAAAAGCAGCTG231810.23984728243437595No Hit
TATTGCACTTGTCCCGGCCTGTAG225710.2335357841260644No Hit
TGAGGTAGTAGTTTGTGCTGTT218500.22607580006001096No Hit
TTATAAAGCAATGAGACTGATT217790.22534118304379766No Hit
TAGCTTATCAGACTGATGTTGACA216920.2244410185309729No Hit
CACTAGATTGTGAGCTCCTGGAA216480.22398576291529143No Hit
TGATGTAGTAGATTGTATAGTT212340.21970222144047016No Hit
TGTAAACATCCTACACTCTCAGCT208470.21569804136618073No Hit
TCGTACGACTCTTAGCGG201530.20851741870065907No Hit
TAGCAGCACGTAAATATTGGC193120.19981582841001977No Hit
CCCCCACTGCTAAATTTGACTGGCTT181560.18785502177984253No Hit
TATTGCACTTGTCCCGGCCTGTAAT177080.18321969187472192No Hit
CAACGGAATCCCAAAAGCAGCTGA175130.18120208175976987No Hit
CGCGACCTCAGATCAGACGTGG171840.17779801136069695No Hit
CTTCTCACTACTGCACTTGACTAG169610.17549069312667487No Hit
TATTGCACTTGTCCCGGCCTGTT167750.17356620347856677No Hit
TGAATACCGGGTGCTGTAGGCTTT166640.17241771772082484No Hit
CAAAGAATTCTCCTTTTGGGCT161140.16672702252480626No Hit
TTCAAGTAATCCAGGATAGGCTT157960.16343676602965368No Hit
TCAGTGCACTACAGAACTTTGT155440.16082939295802334No Hit
ACTGGACTTGGAGTCAGAAGGCT150260.15546979275522768No Hit
AGCAGCATTGTACAGGGCTATGT144970.14999637864851162No Hit
CGCGACCTCAGATCAGACGTGGCG140740.14561971670684642No Hit
TTCACAGTGGCTAAGTTCCGC139550.14438845720079876No Hit
ACTGGACTTGGAGTCAGAAGGCA138990.1438090409626587No Hit
CAAAGAATTCTCCTTTTGGGCTT138470.14327101159867148No Hit
ACCCCCCACTGCTAAATTTGACTG136560.14129478835787232No Hit
TATTGCACTTGTCCCGGCCTGTAAA129990.13449699427826464No Hit
TGTAAACATCCCCGACTGGAAGC129260.1337416838249749No Hit
ACTTGACTTGGAGTCAGAAGGC128930.1334002421132138No Hit
AATGCACCCGGGCAAGGATTCT128580.13303810696437623No Hit
ACCACAGGGTAGAACCACGGAC126740.13113431075334456No Hit
TTCACAGTGGCTAAGTTCTGC121680.12589887117300746No Hit
TGAGGTAGTAGGTTGTGTGGTT119360.12349843247214147No Hit
CTCCCACTGCTTCACTTGACTAG119330.12346739231652681No Hit
TGAGAACTGAATTCCATAGGCTGT118370.12247410733685811No Hit
TACCACCCTGAACGCGCCCGA114050.11800432492834896No Hit
TGAATACCGGGTGCTGTAGGCTT113190.11711450713406243No Hit
TGAGGTAGTAGGTTGTATAGT112060.11594532793924407No Hit
TGAGGTAGTAAGTTGTATTGTT111690.11556249935333009No Hit
CGCCTGTCTGAGCGTCGCT111390.11525209779718364No Hit
ATGCACCTGGGCAAGGATTCTGA110860.1147037217146582No Hit
AGCAGCATTGTACAGGGCTATG110570.11440366687704995No Hit
CAAAGTGCTGTTCGTGCAGGTAG110370.11419673250628562No Hit
AGCTCGGTCTGAGGCCCCTCAGT110120.11393806454283023No Hit
TTCACAGTGGCTAAGTTCTG109800.11360696954960735No Hit
TCAGTGCACTACAGAACTTTGTC109590.11338968846030481No Hit
ACCGGGTGCTGTAGGCTT109410.11320344752661694No Hit
CCCCCCACTGCTAAATTTGACTGG109410.11320344752661694No Hit
CCCATAAAGTAGAAAGCACTA105020.10866123808834027No Hit
AACCGGGCGGAAACACCA104930.10856811762149635No Hit
TTCAAGTAATCCAGGATAGGC103070.10664362797338824No Hit
ACTGGACTTGGAGTCAGAAGG102660.1062194125133214No Hit
CCCCCCACTGCTAAATTTGACTGGCT101470.10498815300727374No Hit
GGAATACCAGGTGCTGTAGGCTTT99650.1031050502333185No Hit
AACATTCAACGCTGTCGGTGA97120.10048733044314996No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)