FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12551213
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG7471545.952842964261701No Hit
CCCCCCACTGCTAAATTTGACTG5875524.681236785639762No Hit
AACATTCAACGCTGTCGGTGAGT4835933.852958275825612No Hit
AAGCTGCCAGTTGAAGAACTGT4493983.5805144889183222No Hit
TAGCAGCACGTAAATATTGGCG4109313.274034151121489No Hit
TTCAAGTAATCCAGGATAGGCT3956933.1526275587865493No Hit
GCCTGTCTGAGCGTCGCT3369412.6845293757663105No Hit
TAGCTTATCAGACTGATGTTGAC2193091.7473131879763335No Hit
AACATTCAACGCTGTCGGTGAG2180211.7370512316219955No Hit
CCCCCACTGCTAAATTTGACTG2162721.7231163234979758No Hit
TAGCTTATCAGACTGATGTTGACT1775611.4146919504911597No Hit
TATTGCACTTGTCCCGGCCTGTAA1750711.3948532305204284No Hit
TATTGCACTTGTCCCGGCCTGTAT1496691.1924664173892994No Hit
CATAAAGTAGAAAGCACTACT1426891.1368542626119085No Hit
TGAGGTAGTAGGTTGTATAGTT1390111.1075503220286358No Hit
TCCTGTCTGAGCGTCGCT1373621.0944121496464125No Hit
TATTGCACTTGTCCCGGCCTGT1323761.0546869055604426No Hit
TAGCTTATCAGACTGATGTTGA1306811.041182234736993No Hit
TGTAAACATCCTTGACTGGAAGCT1302251.0375491197544016No Hit
AGCAGCATTGTACAGGGCTATGA936210.745911968827236No Hit
CGCCTGTCTGAGCGTCGCT845460.67360820025921No Hit
TATTGCACTTGTCCCGGCCTGTA795790.6340343359641812No Hit
TGAGGTAGTAGATTGTATAGTT780690.6220036262630552No Hit
TGTAAACATCCCCGACTGGAAGCT758560.6043718642971002No Hit
CCCATAAAGTAGAAAGCACTAC696880.5552292037430964No Hit
ACTGGACTTGGAGTCAGAAGGC691330.5508073203761262No Hit
TTCAAGTAATTCAGGATAGGTT675960.538561492024715No Hit
CAACGGAATCCCAAAAGCAGCTGT538140.42875537209033104No Hit
CATTGCACTTGTCTCGGTCTGA493490.3931811212191204No Hit
TGTAAACATCCTACACTCTCAGCT466030.3713027577493905No Hit
AACATTCAACGCTGTCGGTGAGTT431470.3437675705129058No Hit
TTCTCACTACTGCACTTGACTAG406810.32412006712020586No Hit
CCCATAAAGTAGAAAGCACT369940.29474442032017145No Hit
TCGTACGACTCTTAGCGG364480.29039424316996293No Hit
TGAGGTAGTAGTTTGTACAGTT347260.2766744536962284No Hit
TGTCAGTTTGTCAAATACCCCAA317380.25286798973135105No Hit
CACCCGGCTGTGTGCACATGTGC316740.25235807885660133No Hit
CTTCTCACTACTGCACTTGACTAG293540.23387380964692417No Hit
TACGCCTGTCTGAGCGTCGCT290040.23108523455063665No Hit
TGATGTAGTAGGTTGTATAGTT280410.22341266935713705No Hit
CTACGCCTGTCTGAGCGTCGCT276280.2201221507435178No Hit
CAACACCAGTCGATGGGCTGT255610.20365362296058553No Hit
CGCGACCTCAGATCAGACGTGGCG255090.2032393203748514No Hit
CCCCCACTGCTAAATTTGACTGGCTT241880.19271444122572057No Hit
TAGCTTATCAGACTGATGTTGACA239900.19113690445696363No Hit
TTATAAAGCAATGAGACTGATT237120.18892197909476957No Hit
TTCAAGTAATCCAGGATAGGCTT234530.1868584335235168No Hit
CAAAGAATTCTCCTTTTGGGCT231210.18421327086075268No Hit
CAAAGAATTCTCCTTTTGGGCTT229390.18276321181068317No Hit
TGAGGTAGTAGTTTGTGCTGTT229220.18262776673457776No Hit
TGTAAACATCCCCGACTGGAAGC226780.18068373152459447No Hit
TTAATGCTAATCGTGATAGGGGTT225580.17972764863443874No Hit
ACGCCTGTCTGAGCGTCGCT219900.17520218962103504No Hit
TATTGCACTTGTCCCGGCCTGTT217890.1736007507800242No Hit
TGTAAACATCCTACACTCAGCT210570.16776864515007434No Hit
CCCCCCACTGCTAAATTTGACTGGCT208720.16629468402775094No Hit
CACTAGATTGTGAGCTCCTGGA208290.16595208765877847No Hit
TGTCAGTTTGTCAAATACCCCA204850.16321131670699876No Hit
CTCCCACTGCTTCACTTGACTAG203310.16198434366463227No Hit
TATTGCACTTGTCCCGGCCTGTAG201910.16086891362611724No Hit
TAGCAGCACGTAAATATTGGC201360.16043070896812922No Hit
CAACGGAATCCCAAAAGCAGCT201180.16028729653460586No Hit
TTCACAGTGGCTAAGTTCCG200670.15988096130628968No Hit
GCGTTCGCGCTTTCCCCTG193480.15415243132277334No Hit
TTCAAGTAATCCAGGATAGGC185080.14745985109168333No Hit
AGCAGCATTGTACAGGGCTATGT184330.146862299285336No Hit
TTCACAGTGGCTAAGTTCCGC182940.14575483660423896No Hit
ACCACAGGGTAGAACCACGGAC180800.1440498221167946No Hit
TAGCAGCACGTAAATATTGGCGT179490.1430060982950413No Hit
ACTGGACTTGGAGTCAGAAGGCA178140.1419305050436161No Hit
CGCGACCTCAGATCAGACGTGG176810.14087084650702686No Hit
CCCCCCACTGCTAAATTTGACTGG176480.14060792371223405No Hit
AGCTCGGTCTGAGGCCCCTCAGT175570.13988289418719926No Hit
AACGGAATCCCAAAAGCAGCTG173380.1381380429126651No Hit
CAAAGTGCTGTTCGTGCAGGTAG168190.1340029844127416No Hit
CACTAGATTGTGAGCTCCTGGAA166960.133022999450332No Hit
GTCAGGATGGCCGAGCGGTCTAAGG163340.13013881606502894No Hit
ACCGGGTGCTGTAGGCTT160330.12774064148222167No Hit
AACCGGGCGGAAACACCA154620.12319128039656405No Hit
TTCACAGTGGCTAAGTTCTGC153660.12242641408443948No Hit
ACTGGACTTGGAGTCAGAAGG151940.12105602860854962No Hit
TGAGGTAGTAGGTTGTGTGGTT151010.12031506436867895No Hit
CCAAGTCCTTCTGATCGAGGCCC147110.11720779497567287No Hit
AGCAGCATTGTACAGGGCTATG146040.1163552877319507No Hit
AACATTCAACGCTGTCGGTGA143800.11457059967032669No Hit
CAAAGTGCTGTTCGTGCAGGT138000.10994953236790739No Hit
AACATTCAACGCTGTCGGTGAGA137480.10953522978217325No Hit
GGAATACCGGGTGCTGTAGGCTTT137070.1092085681280367No Hit
TATTGCACTTGTCCCGGCCTGTAAT136700.10891377590357204No Hit
ATCCCGGACGAGCCCCCA136050.10839589767140435No Hit
ACCCCCCACTGCTAAATTTGACTG134590.10723266348838156No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)