FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10560739
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5647175.347324652185799No Hit
CCCCCCACTGCTAAATTTGACTG4696924.447529666247788No Hit
AAGCTGCCAGTTGAAGAACTGT4498044.259209511758599No Hit
TTCAAGTAATCCAGGATAGGCT4318264.088975212814179No Hit
TAGCAGCACGTAAATATTGGCG4252824.0270098522461355No Hit
AACATTCAACGCTGTCGGTGAGT3543313.3551723984467374No Hit
CATAAAGTAGAAAGCACTACT2577452.4405962499404636No Hit
TAGCTTATCAGACTGATGTTGAC2397352.270058941897911No Hit
CCCCCACTGCTAAATTTGACTG2101901.990296323013001No Hit
TAGCTTATCAGACTGATGTTGA1873951.7744496857653618No Hit
TAGCTTATCAGACTGATGTTGACT1813891.7175786656596665No Hit
AACATTCAACGCTGTCGGTGAG1556081.473457491942562No Hit
TGAGGTAGTAGGTTGTATAGTT1408121.3333536601936664No Hit
TGTAAACATCCTTGACTGGAAGCT1257791.1910056673117289No Hit
TATTGCACTTGTCCCGGCCTGT964940.9137049973491438No Hit
TTCAAGTAATTCAGGATAGGTT878220.8315895317553063No Hit
TATTGCACTTGTCCCGGCCTGTAA875780.8292790873820477No Hit
TGAGGTAGTAGATTGTATAGTT846590.8016389762117974No Hit
TATTGCACTTGTCCCGGCCTGTAT775600.7344183015980227No Hit
CGCCTGTCTGAGCGTCGCT724620.6861451646518297No Hit
AGCAGCATTGTACAGGGCTATGA692410.6556454051179562No Hit
CCCATAAAGTAGAAAGCACTAC613440.5808684411195089No Hit
TGTAAACATCCCCGACTGGAAGCT538600.5100021882938306No Hit
TATTGCACTTGTCCCGGCCTGTA507980.48100800521630166No Hit
TGATGTAGTAGGTTGTATAGTT506420.4795308358629069No Hit
CATTGCACTTGTCTCGGTCTGA461110.43662664137424473No Hit
ACTGGACTTGGAGTCAGAAGGC443410.41986645063380507No Hit
TGTAAACATCCTACACTCTCAGCT398400.37724632717464185No Hit
TGTCAGTTTGTCAAATACCCCAA396120.3750873873504496No Hit
CAACGGAATCCCAAAAGCAGCTGT388720.36808030195614155No Hit
TGAGGTAGTAGTTTGTACAGTT377980.35791055910007813No Hit
TTATAAAGCAATGAGACTGATT374520.35463427322652324No Hit
CCCATAAAGTAGAAAGCACT372960.3531571038731286No Hit
TCGTACGACTCTTAGCGG318870.301939097254463No Hit
CAACACCAGTCGATGGGCTGT317240.3003956446608519No Hit
TTCTCACTACTGCACTTGACTAG308180.29181669956998274No Hit
TGATGTAGTAGATTGTATAGTT304210.28805749294627964No Hit
AACATTCAACGCTGTCGGTGAGTT301630.28561448209258844No Hit
TGTCAGTTTGTCAAATACCCCA267850.2536280841710036No Hit
TGTAAACATCCTACACTCAGCT262050.2481360442673567No Hit
CTACGCCTGTCTGAGCGTCGCT258150.24444312088386996No Hit
TTCAAGTAATCCAGGATAGGCTT247120.23399877603262426No Hit
TTCACAGTGGCTAAGTTCCG245150.2321333762722476No Hit
TACGCCTGTCTGAGCGTCGCT231930.21961531290565933No Hit
TGCAAGTAATCCAGGATAGGCT226460.21443575113446134No Hit
TGAGGTAGTAGTTTGTGCTGTT221480.2097201720447783No Hit
TTCACAGTGGCTAAGTTCCGC216420.20492884068056225No Hit
TAGCAGCACGTAAATATTGGC206090.19514732823148076No Hit
ACGCCTGTCTGAGCGTCGCT205870.19493900947651485No Hit
TAGCAGCACGTAAATATTGGCGT203850.19302626454455507No Hit
TTCAAGTAATCCAGGATAGGC202720.1919562636667756No Hit
TAGCTTATCAGACTGATGTTGACA199820.18921024371495215No Hit
ACCGGGTGCTGTAGGCTT195800.18540369191966585No Hit
ACTTGACTTGGAGTCAGAAGGC188900.17887005824118937No Hit
CTTCTCACTACTGCACTTGACTAG188150.17815988066744193No Hit
CAAAGAATTCTCCTTTTGGGCT182240.1725636813863121No Hit
CAAAGAATTCTCCTTTTGGGCTT175110.16581225991855306No Hit
CATAAAGTAGAAAGCACTAC171410.16230871722139897No Hit
TAGCAGCACATAATGGTTTGT170460.1614091589613189No Hit
CACCCGGCTGTGTGCACATGTGC170050.16102092855433695No Hit
TGTAAACATCCCCGACTGGAAGC167100.15822756343093036No Hit
CAACGGAATCCCAAAAGCAGCT165920.15711021738156772No Hit
CCCCCACTGCTAAATTTGACTGGCTT164680.1559360571263053No Hit
TTTAAACATCCTTGACTGGAAGCT162110.1535025153069307No Hit
TTAATGCTAATCGTGATAGGGGTT158170.14977171578617746No Hit
TGTCAGTTTGTCAAATACCCC154440.14623976598607352No Hit
AGCAGCATTGTACAGGGCTATGT151130.14310551562726814No Hit
TTCACAGTGGCTAAGTTCTGC150450.14246162129373713No Hit
ACCACAGGGTAGAACCACGGAC148620.14072878801379335No Hit
TATTGCACTTGTCCCGGCCTGTT146650.13886338825341674No Hit
TGATGTAGTAGTTTGTACAGTT139160.13177108155025893No Hit
CAAAGTGCTGTTCGTGCAGGT137400.1301045315105316No Hit
CACTAGATTGTGAGCTCCTGGA134890.12772780389705682No Hit
TTCACAGTGGCTAAGTTCTG131350.12437576574896889No Hit
AGCAGCATTGTACAGGGCTATG131260.1242905444401192No Hit
CGCGACCTCAGATCAGAC127930.12113735601268055No Hit
TGAGGTAGTAGGTTGTATAGT127660.12088169208613146No Hit
AACATTCAACGCTGTCGGTGA122390.1158915110012661No Hit
ACCGGGTGCTGTAGGCTTT121810.11534230701090141No Hit
GCGTTCGCGCTTTCCCCTG120840.11442381068218804No Hit
CCCCCCACTGCTAAATTTGACTGGCT118470.11217964954914614No Hit
GCCTGTCTGAGCGTCGCT118240.11196186175986358No Hit
CAAAGTGCTGTTCGTGCAGGTAG117870.11161150749014817No Hit
ATCCCGGACGAGCCCCCA117650.11140318873518226No Hit
AACCGGGCGGAAACACCA116680.1104846924064689No Hit
TGTCAGTTTGTCAAATACC115970.109812390969988No Hit
AACGGAATCCCAAAAGCAGCTG113970.10791858410666147No Hit
CTCCCACTGCTTCACTTGACTAG113410.10738831818493004No Hit
CCCCCCACTGCTAAATTTGACTGG110120.10427300589475794No Hit
TGAGAACTGAATTCCATAGGCTGT109720.10389424452209263No Hit
TTAGGTAGTAGGTTGTATAGTT109090.10329769536014477No Hit
TCAGTGCACTACAGAACTTTGT108980.10319353598266183No Hit
CATAAAGTAGAAAGCACTACTA108490.10272955330114682No Hit
TGTAAACATCCTTGACTGGAAGC108450.1026916771638803No Hit
ACTGGACTTGGAGTCAGAAGG107960.10222769448236531No Hit
CCCATAAAGTAGAAAGCACTA106890.1012145078104856No Hit
TGTCAGTTTGTCAAATACCC105870.10024866631018908No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)