FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11409762
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG9079137.9573351310921305No Hit
CAACGGAATCCCAAAAGCAGCTG6530985.724028248792569No Hit
TAGCAGCACGTAAATATTGGCG3981033.4891437700453345No Hit
AAGCTGCCAGTTGAAGAACTGT3788593.3204811809396197No Hit
TTCAAGTAATCCAGGATAGGCT3752393.2887539634919647No Hit
CCCCCACTGCTAAATTTGACTG3289212.8828033398067374No Hit
AACATTCAACGCTGTCGGTGAGT2974402.606890485533353No Hit
TAGCTTATCAGACTGATGTTGAC2820092.4716466478441883No Hit
TAGCTTATCAGACTGATGTTGACT2413962.1156970671255015No Hit
AACATTCAACGCTGTCGGTGAG1705371.494658696649413No Hit
TAGCTTATCAGACTGATGTTGA1666011.4601619209936194No Hit
TGTAAACATCCTTGACTGGAAGCT1610451.4114667773087641No Hit
TATTGCACTTGTCCCGGCCTGTAA1551771.3600371331146084No Hit
TATTGCACTTGTCCCGGCCTGTAT1252621.0978493679359833No Hit
CATAAAGTAGAAAGCACTACT1215151.0650090685502467No Hit
TGAGGTAGTAGGTTGTATAGTT1042880.9140243240831841No Hit
TATTGCACTTGTCCCGGCCTGT856970.7510849043126403No Hit
AGCAGCATTGTACAGGGCTATGA838360.7347743099286383No Hit
TTCAAGTAATTCAGGATAGGTT814710.7140464454911505No Hit
TGAGGTAGTAGATTGTATAGTT717990.6292769297028281No Hit
TATTGCACTTGTCCCGGCCTGTA717330.6286984776720146No Hit
TGTAAACATCCCCGACTGGAAGCT693360.6076901516438292No Hit
TTAATGCTAATCGTGATAGGGGTT630620.5527021510176987No Hit
TGTAAACATCCTACACTCTCAGCT508750.4458901070854939No Hit
CCCATAAAGTAGAAAGCACTAC449440.3939083041346524No Hit
CAACGGAATCCCAAAAGCAGCTGT432290.37887731575820777No Hit
GCCTGTCTGAGCGTCGCT430840.37760647417535964No Hit
TTCTCACTACTGCACTTGACTAG411490.3606473123628696No Hit
CGCCTGTCTGAGCGTCGCT409160.35860520140560337No Hit
ACTGGACTTGGAGTCAGAAGGC408700.3582020378689757No Hit
TGATGTAGTAGGTTGTATAGTT372490.32646605599661066No Hit
CAAAGAATTCTCCTTTTGGGCT360250.3157384001524309No Hit
CATTGCACTTGTCTCGGTCTGA357620.3134333564538857No Hit
TAGCTTATCAGACTGATGTTGACA344260.30172408504226467No Hit
AACATTCAACGCTGTCGGTGAGTT317150.27796372965536004No Hit
CCCCCACTGCTAAATTTGACTGGCTT309410.271180064930364No Hit
CTTCTCACTACTGCACTTGACTAG307770.2697426992780393No Hit
TGAGAACTGAATTCCATAGGCTGT285670.2503733206704925No Hit
CCCATAAAGTAGAAAGCACT276980.24275703559811326No Hit
TGAGGTAGTAGTTTGTACAGTT269380.2360960728190474No Hit
TTCACAGTGGCTAAGTTCCG264770.23205567302806143No Hit
TGATGTAGTAGATTGTATAGTT256230.2245708543263216No Hit
TTCAAGTAATCCAGGATAGGCTT232670.20392186971121745No Hit
CAAAGAATTCTCCTTTTGGGCTT232440.20372028794290362No Hit
CAACGGAATCCCAAAAGCAGCT223770.19612153171994295No Hit
TTATAAAGCAATGAGACTGATT222890.1953502623455248No Hit
TAGCAGCACGTAAATATTGGC221760.19435988235337426No Hit
CACCCGGCTGTGTGCACATGTGC214140.1876813907248898No Hit
CAACACCAGTCGATGGGCTGT208960.18314141872547385No Hit
TGCAAGTAATCCAGGATAGGCT196870.17254522925193358No Hit
TGTAAACATCCTACACTCAGCT191990.16826818999379653No Hit
TTTAAACATCCTTGACTGGAAGCT191790.1680929014996106No Hit
CCCCCCACTGCTAAATTTGACTGG184880.16203668402548624No Hit
ACCCCCCACTGCTAAATTTGACTG184630.16181757340775382No Hit
CCCCCCACTGCTAAATTTGACTGGCT179100.15697084654351248No Hit
TATTGCACTTGTCCCGGCCTGTT178780.15669038495281495No Hit
TATTGCACTTGTCCCGGCCTGTAG174810.15321090834322398No Hit
CTCCCACTGCTTCACTTGACTAG172970.1515982541967133No Hit
TCCTGTCTGAGCGTCGCT171270.1501083019961328No Hit
AGCTCGGTCTGAGGCCCCTCAGT168990.14811001316241304No Hit
TGTAAACATCCCCGACTGGAAGC165720.14524404628247287No Hit
ACTTGACTTGGAGTCAGAAGGC164310.14400826239846196No Hit
AGCAGCATTGTACAGGGCTATGT156840.13746123714061695No Hit
CTACGCCTGTCTGAGCGTCGCT155930.13666367449207092No Hit
TGAGGTAGTAGTTTGTGCTGTT155170.13599757821416433No Hit
TGTCAGTTTGTCAAATACCCCA153100.13418334229933981No Hit
TCGTACGACTCTTAGCGG146840.12869681243131978No Hit
CGCCCCACTGCTAAATTTGACTG146270.12819724022288984No Hit
TTCAAGTAATCCAGGATAGGC145450.12747855739672745No Hit
TAGCAGCACGTAAATATTGGCGT144660.126786167844693No Hit
TTCACAGTGGCTAAGTTCTG138920.1217553880615564No Hit
ACCGGGTGCTGTAGGCTT137560.120563426301092No Hit
TACGCCTGTCTGAGCGTCGCT136970.12004632524324346No Hit
CCCGCCACTGCTAAATTTGACTG135730.1189595365792906No Hit
CTCCCCACTGCTAAATTTGACTG135120.11842490667202349No Hit
ATCACATTGCCAGGGATTTCCA134080.11751340650225657No Hit
CACTAGATTGTGAGCTCCTGGAA132570.11618997837115269No Hit
TGTAAACATCCTTGACTGGAAGC129310.11333277591592184No Hit
TATTGCACTTGTCCCGGCCTGTAAT129220.11325389609353814No Hit
CAAAGTGCTGTTCGTGCAGGTAG128160.11232486707435266No Hit
TGTCAGTTTGTCAAATACC127370.11163247752231818No Hit
AGCAGCATTGTACAGGGCTATG124650.10924855400138934No Hit
CACTAGATTGTGAGCTCCTGGA124520.10913461648016846No Hit
CGCGACCTCAGATCAGACGTGGCG123820.10852110675051768No Hit
TGTCAGTTTGTCAAATACCCCAA121640.10661046216389088No Hit
TTCACAGTGGCTAAGTTCTGC119050.10434047616418292No Hit
CAGTGCAATAGTATTGTCAAAGC118570.10391978377813665No Hit
AACGGAATCCCAAAAGCAGCTG117340.1028417595388931No Hit
TATTGCACTTGTCCCGGCCTGTAAA116650.1022370142339516No Hit
ATCACATTGCCAGGGATTTCC115080.10086099955459193No Hit
ACCACAGGGTAGAACCACGGAC114140.10003714363191801No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)