FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11733657
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG8654537.375816422791291No Hit
CCCCCCACTGCTAAATTTGACTG7018395.981417387605586No Hit
TAGCAGCACGTAAATATTGGCG4092883.488153778485258No Hit
AAGCTGCCAGTTGAAGAACTGT3931563.350668934672285No Hit
AACATTCAACGCTGTCGGTGAGT3723583.173418142357493No Hit
TTCAAGTAATCCAGGATAGGCT3495852.979335427991461No Hit
CCCCCACTGCTAAATTTGACTG2392242.0387846687524616No Hit
AACATTCAACGCTGTCGGTGAG1805001.538309838100773No Hit
TAGCTTATCAGACTGATGTTGAC1581531.3478577054025016No Hit
TATTGCACTTGTCCCGGCCTGTAA1307801.1145715270183882No Hit
TAGCTTATCAGACTGATGTTGACT1088060.9272982839024526No Hit
TATTGCACTTGTCCCGGCCTGTAT1069560.9115316733734419No Hit
CATAAAGTAGAAAGCACTACT1060800.9040659702256509No Hit
TGTAAACATCCTTGACTGGAAGCT1040460.8867312211359171No Hit
AACCGGGCGGAAACACCA1027050.8753025591254286No Hit
TAGCTTATCAGACTGATGTTGA1015440.8654079457069523No Hit
TATTGCACTTGTCCCGGCCTGT959530.8177586919406286No Hit
TGAGGTAGTAGGTTGTATAGTT951130.81059979851124No Hit
GGAATACCGGGTGCTGTAGGCTTT912210.7774302589550726No Hit
AGCAGCATTGTACAGGGCTATGA828990.706506079051058No Hit
GGAATACCGGGTGCTGTAGGCTT668690.5698905294402248No Hit
TTCAAGTAATTCAGGATAGGTT648870.5529989499437388No Hit
TTCTCACTACTGCACTTGACTAG636420.5423884471823235No Hit
CGCCTGTCTGAGCGTCGCT628070.535272166213824No Hit
TATTGCACTTGTCCCGGCCTGTA616240.525190057967435No Hit
TGTAAACATCCCCGACTGGAAGCT610660.5204345073321983No Hit
TGAGGTAGTAGATTGTATAGTT588370.5014378722677849No Hit
CAACGGAATCCCAAAAGCAGCTGT561660.4786742956607646No Hit
CCCATAAAGTAGAAAGCACTAC560240.4774641017715108No Hit
GCCTGTCTGAGCGTCGCT549980.4687200247970432No Hit
TGAATACCGGGTGCTGTAGGCTTT530260.4519136702223357No Hit
CCCCCACTGCTAAATTTGACTGGCTT485800.41402266999964293No Hit
ACTGGACTTGGAGTCAGAAGGC454320.3871938646237912No Hit
CTTCTCACTACTGCACTTGACTAG403720.34407005420390246No Hit
TGTAAACATCCTACACTCTCAGCT403050.34349904722798696No Hit
TGAATACCGGGTGCTGTAGGCTT390690.33296524689617224No Hit
CTACGCCTGTCTGAGCGTCGCT379990.3238461802658796No Hit
TGTCAGTTTGTCAAATACCCCAA378120.32225247422862285No Hit
AACATTCAACGCTGTCGGTGAGTT362780.30917897122781074No Hit
CCCCCCACTGCTAAATTTGACTGGCT361270.30789207490895637No Hit
CATTGCACTTGTCTCGGTCTGA341990.29146070999007384No Hit
CTCCCACTGCTTCACTTGACTAG314070.2676659118295345No Hit
TGATGTAGTAGGTTGTATAGTT313450.2671375173145082No Hit
TCCTGTCTGAGCGTCGCT286460.24413531092650825No Hit
TTCACAGTGGCTAAGTTCCG263610.22466141630013559No Hit
TGAGGTAGTAGTTTGTACAGTT261890.22319554764554647No Hit
CCCATAAAGTAGAAAGCACT260530.22203648870935977No Hit
TGTCAGTTTGTCAAATACCCCA251560.21439181322583403No Hit
TACGCCTGTCTGAGCGTCGCT250110.2131560518600467No Hit
CACCCGGCTGTGTGCACATGTGC241490.20580966360274552No Hit
CCCCCCACTGCTAAATTTGACTGG238640.20338075333206007No Hit
CAAAGAATTCTCCTTTTGGGCT238160.2029716737075236No Hit
TAGCAGCACGTAAATATTGGC226590.19311115025775852No Hit
ACGCCTGTCTGAGCGTCGCT224650.19145778677525685No Hit
TAGCTTATCAGACTGATGTTGACA219310.18690677595228836No Hit
TGTAAACATCCCCGACTGGAAGC216470.18448638817378077No Hit
GTCAGGATGGCCGAGCGGTCTAAGG206360.17587014858198088No Hit
TGTAAACATCCTACACTCAGCT205070.17477074709103907No Hit
CAACGGAATCCCAAAAGCAGCT196650.16759480867729473No Hit
TGATGTAGTAGATTGTATAGTT194270.16556645553896793No Hit
AGCTCGGTCTGAGGCCCCTCAGT182640.15565479713613584No Hit
CAAAGAATTCTCCTTTTGGGCTT180850.1541292710363018No Hit
TGAGGTAGTAGTTTGTGCTGTT177660.1514105960315697No Hit
TTCAAGTAATCCAGGATAGGC171300.14599029100646116No Hit
TATTGCACTTGTCCCGGCCTGTT170330.14516360926521032No Hit
CTCCCACTGCTTCACTTGACTAGCCT170270.14511247431214327No Hit
TTCAAGTAATCCAGGATAGGCTT169700.14462669225800617No Hit
TAGCAGCACGTAAATATTGGCGT166630.1420102871594082No Hit
ACTTGACTTGGAGTCAGAAGGC161950.13802176082017736No Hit
GGAATACCAGGTGCTGTAGGCTTT160010.13636839733767572No Hit
AACGGAATCCCAAAAGCAGCTG159210.13568659796344823No Hit
ACCCCCCACTGCTAAATTTGACTG158240.1348599162221974No Hit
ATCACATTGCCAGGGATTTCCA143900.12263866243916964No Hit
TTCACAGTGGCTAAGTTCTGC142560.12149664848733861No Hit
GGAATACCGGGTGCTGTAGGCTTA142460.12141142356556017No Hit
CAACACCAGTCGATGGGCTGT142290.12126654119853683No Hit
TATTGCACTTGTCCCGGCCTGTAG141700.12076371416004404No Hit
CCCCCACTGCTAAATTTGACTGGCT141360.12047394942599737No Hit
AGCAGCATTGTACAGGGCTATG140010.11932341298198847No Hit
CAAAGTGCTGTTCGTGCAGGTAG138790.11828366893629155No Hit
TTCACAGTGGCTAAGTTCCGC138620.11813878656926821No Hit
CCAAGTCCTTCTGATCGAGGCCC137290.11700529510961502No Hit
AGCAGCATTGTACAGGGCTATGT136840.11662178296161206No Hit
GGGCTACGCCTGTCTGAGCGTCGCT135890.1158121462047169No Hit
CAAAGTGCTGTTCGTGCAGGT132570.11298267880167283No Hit
ACCACAGGGTAGAACCACGGAC132350.11279518397376026No Hit
TTATAAAGCAATGAGACTGATT131450.11202815967775435No Hit
TGAGGTAGTAGGTTGTGTGGTT125710.1071362491676721No Hit
ACCGGGTGCTGTAGGCTT125570.1070169342771823No Hit
ATCACATTGCCAGGGATTTCC121460.10351418999208857No Hit
CACTAGATTGTGAGCTCCTGGA121030.10314772282844129No Hit
ACTGGACTTGGAGTCAGAAGGCA120250.1024829684385695No Hit
TCGTACGACTCTTAGCGG117540.10017337305837387No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)