FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10224334
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG5760415.6340197806527055No Hit
CAACGGAATCCCAAAAGCAGCTG4992314.882772804566049No Hit
AAGCTGCCAGTTGAAGAACTGT4205124.112854685693954No Hit
TTCAAGTAATCCAGGATAGGCT3354353.2807515873405544No Hit
TAGCAGCACGTAAATATTGGCG2841882.7795257862272496No Hit
TAGCTTATCAGACTGATGTTGAC2687102.6281418427840872No Hit
AACATTCAACGCTGTCGGTGAGT2529522.4740193346578856No Hit
TAGCTTATCAGACTGATGTTGA2333652.282446954491119No Hit
CCCCCACTGCTAAATTTGACTG2125862.079216113245127No Hit
TAGCTTATCAGACTGATGTTGACT1965271.9221496480846576No Hit
CATAAAGTAGAAAGCACTACT1456771.4248067404683766No Hit
TGAGGTAGTAGGTTGTATAGTT1434661.4031818600605184No Hit
AACATTCAACGCTGTCGGTGAG1320321.2913506151109695No Hit
TGAGGTAGTAGATTGTATAGTT1188241.1621686067767347No Hit
TGTAAACATCCTTGACTGGAAGCT1145571.1204348371248436No Hit
GCCTGTCTGAGCGTCGCT1010800.9886218505772602No Hit
TATTGCACTTGTCCCGGCCTGTAA847790.8291884830835925No Hit
TATTGCACTTGTCCCGGCCTGTAT847470.8288755042626738No Hit
TATTGCACTTGTCCCGGCCTGT843050.8245524842987328No Hit
TTCAAGTAATTCAGGATAGGTT838800.820395734333405No Hit
CGCCTGTCTGAGCGTCGCT712820.6971798847729348No Hit
AGCAGCATTGTACAGGGCTATGA618670.6050956473057316No Hit
TGAGGTAGTAGTTTGTACAGTT483150.4725491166466197No Hit
TTCTCACTACTGCACTTGACTAG479400.46888139608897755No Hit
TGTAAACATCCCCGACTGGAAGCT455390.44539820393191376No Hit
TATTGCACTTGTCCCGGCCTGTA433980.42445796469481534No Hit
TGTCAGTTTGTCAAATACCCCAA412840.40378180133786706No Hit
CCCATAAAGTAGAAAGCACTAC401420.3926123696663274No Hit
ACTGGACTTGGAGTCAGAAGGC397100.3883871555839236No Hit
TGTAAACATCCTACACTCTCAGCT378620.3703126286758629No Hit
CATTGCACTTGTCTCGGTCTGA353050.34530366476682006No Hit
ACCGGGTGCTGTAGGCTT340120.3326573642840698No Hit
TGTCAGTTTGTCAAATACCCCA330150.3229061178948184No Hit
CTTCTCACTACTGCACTTGACTAG322690.31560979913214887No Hit
AACCGGGCGGAAACACCA309970.30316889100062655No Hit
CAACGGAATCCCAAAAGCAGCTGT305780.299070824564221No Hit
CAACACCAGTCGATGGGCTGT295910.28941738405650674No Hit
CCCATAAAGTAGAAAGCACT295600.28911418582374165No Hit
CACCCGGCTGTGTGCACATGTGC266860.2610047754699719No Hit
CTACGCCTGTCTGAGCGTCGCT263970.258178185493549No Hit
TTATAAAGCAATGAGACTGATT260080.25437353670175483No Hit
CTCCCACTGCTTCACTTGACTAG259600.25390406847037666No Hit
TAGCTTATCAGACTGATGTTGACA256370.2507449384967275No Hit
TGAGGTAGTAGTTTGTGCTGTT256100.25048086261657726No Hit
TGATGTAGTAGGTTGTATAGTT238810.23357022569880834No Hit
TACGCCTGTCTGAGCGTCGCT236430.23124244571822478No Hit
ACCGGGTGCTGTAGGCTTA229970.22492418577092652No Hit
TTCACAGTGGCTAAGTTCCG227210.22222474344050186No Hit
ACCGGGTGCTGTAGGCTTT218660.21386234056907766No Hit
TGTAAACATCCTACACTCAGCT215690.21095750588742504No Hit
AACATTCAACGCTGTCGGTGAGTT212200.2075440806217794No Hit
TGATGTAGTAGATTGTATAGTT202550.19810581305344682No Hit
TTCACAGTGGCTAAGTTCCGC199500.1951227336665645No Hit
ACGCCTGTCTGAGCGTCGCT194500.19023243958970823No Hit
CGCGACCTCAGATCAGACGTGGCG191240.18704396785159796No Hit
TTCAAGTAATCCAGGATAGGCTT183320.17929774203385765No Hit
GGAATACCGGGTGCTGTAGGCTTT181970.17797736263310646No Hit
CCCCCACTGCTAAATTTGACTGGCTT177010.17312619090886508No Hit
CAAAGAATTCTCCTTTTGGGCT175360.1715123938635025No Hit
TAGCAGCACGTAAATATTGGC169570.16584943332250296No Hit
TCGTACGACTCTTAGCGG164170.16056791571949822No Hit
GGAATACCGGGTGCTGTAGGCTT163850.16025493689857942No Hit
TAGCAGCACGTAAATATTGGCGT162870.1592964392595156No Hit
GCGTTCGCGCTTTCCCCTG160910.15737944398138792No Hit
TAGCTTATCAGACTGATGTTG153600.15022983404102408No Hit
TGTCAGTTTGTCAAATACCCC151000.14768688112105885No Hit
ACCCCCCACTGCTAAATTTGACTG150340.14704136230291381No Hit
TTCAAGTAATCCAGGATAGGC147870.14462555702894683No Hit
CCCCCCACTGCTAAATTTGACTGG145590.14239558292990037No Hit
CAAAGAATTCTCCTTTTGGGCTT144430.14126103470406973No Hit
TGAGGTAGTAGGTTGTATAGT143940.1407817858845378No Hit
CGCGACCTCAGATCAGACGTGG141200.13810190473042058No Hit
TGTAAACATCCCCGACTGGAAGC139370.1363120570982912No Hit
GGGCTACGCCTGTCTGAGCGTCGCT136060.13307468241941237No Hit
TTCACAGTGGCTAAGTTCTGC134610.13165649713712405No Hit
TATTGCACTTGTCCCGGCCTGTT134240.13129461537543669No Hit
AGCAGCATTGTACAGGGCTATGT130550.12768557834671676No Hit
CCCCCCACTGCTAAATTTGACTGGCT127910.12510350307413667No Hit
TGTCAGTTTGTCAAATACC122290.11960681253175022No Hit
TAGCAGCACATAATGGTTTGT122140.11946010370944456No Hit
TGAGGTAGTAGATTGTATAGT121030.11837445842438246No Hit
AACGGAATCCCAAAAGCAGCTG119990.11735727725639636No Hit
TCAGTGCACTACAGAACTTTGT118260.11566523550580408No Hit
TGAGGTAGTAAGTTGTATTGTT118070.11547940433088355No Hit
TGAGGTAGTAGGTTGTGTGGTT113800.11130309318924832No Hit
ATCCCGGACGAGCCCCCA109810.10740063851591704No Hit
AGCAGCATTGTACAGGGCTATG109140.1067453391096183No Hit
TTCACAGTGGCTAAGTTCTG108730.10634433499531606No Hit
ACTGGACTTGGAGTCAGAAGG107230.10487724677225921No Hit
TAGTGGGTTATCAGAACT106930.10458382912764783No Hit
CAAAGTGCTGTTCGTGCAGGT106670.1043295338356513No Hit
CAAAGTGCTGTTCGTGCAGGTAG106160.10383072383981197No Hit
CAACGGAATCCCAAAAGCAGCT106090.10376225972273598No Hit
AGCTCGGTCTGAGGCCCCTCAGT105870.10354708678335431No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)