FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10359942
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG8638698.338550544008836No Hit
CAACGGAATCCCAAAAGCAGCTG4887054.717256139078771No Hit
AAGCTGCCAGTTGAAGAACTGT3130933.022150124006486No Hit
AACATTCAACGCTGTCGGTGAGT2879602.779552240736483No Hit
CCCCCACTGCTAAATTTGACTG2700292.606472121175968No Hit
TAGCAGCACGTAAATATTGGCG2582972.4932282439419065No Hit
TAGCTTATCAGACTGATGTTGAC2513962.426615901903698No Hit
TTCAAGTAATCCAGGATAGGCT2217962.1409000166217145No Hit
TAGCTTATCAGACTGATGTTGACT1689281.6305882793552318No Hit
AACATTCAACGCTGTCGGTGAG1559441.5052593923788378No Hit
TAGCTTATCAGACTGATGTTGA1170101.1294464775961102No Hit
TGTAAACATCCTTGACTGGAAGCT1122691.083683673132533No Hit
TATTGCACTTGTCCCGGCCTGTAA1071811.034571429067846No Hit
TATTGCACTTGTCCCGGCCTGTAT1001670.9668683473324464No Hit
CGCCTGTCTGAGCGTCGCT938350.9057483140349628No Hit
TGAGGTAGTAGGTTGTATAGTT933250.9008255065520637No Hit
TATTGCACTTGTCCCGGCCTGT792960.7654096905175725No Hit
AGCAGCATTGTACAGGGCTATGA773350.7464810131176409No Hit
ACTGGACTTGGAGTCAGAAGGC647150.6246656593251197No Hit
TGAGGTAGTAGATTGTATAGTT613000.5917021543170802No Hit
TATTGCACTTGTCCCGGCCTGTA534310.5157461306250557No Hit
TGTAAACATCCCCGACTGGAAGCT531160.5127055730620886No Hit
CATAAAGTAGAAAGCACTACT445840.4303498996422953No Hit
TACGCCTGTCTGAGCGTCGCT424610.40985750692426653No Hit
TTCAAGTAATTCAGGATAGGTT410380.39612190879060905No Hit
CGCGACCTCAGATCAGACGTGGCG396600.3828206760230897No Hit
ACCGGGTGCTGTAGGCTT394060.38036892484533213No Hit
CTACGCCTGTCTGAGCGTCGCT342360.33046517055790464No Hit
GCGTTCGCGCTTTCCCCTG326570.3152237724883016No Hit
CAACGGAATCCCAAAAGCAGCTGT310430.29964453468947994No Hit
AACATTCAACGCTGTCGGTGAGTT300260.28982787741475774No Hit
TGTAAACATCCTACACTCTCAGCT292850.2826753277190162No Hit
ACCGGGTGCTGTAGGCTTA291150.2810343918913832No Hit
TCGTACGACTCTTAGCGG289050.2790073535160718No Hit
TGTCAGTTTGTCAAATACCCCAA286390.27643977157401073No Hit
CCCATAAAGTAGAAAGCACTAC285120.27521389598513196No Hit
TTCTCACTACTGCACTTGACTAG274870.2653200182008741No Hit
TAGCTTATCAGACTGATGTTGACA274520.2649821784716555No Hit
CACCCGGCTGTGTGCACATGTGC271340.2619126632176126No Hit
ACCGGGTGCTGTAGGCTTT259030.2500303573128112No Hit
CATTGCACTTGTCTCGGTCTGA255810.24692223180400047No Hit
CCCCCACTGCTAAATTTGACTGGCTT253820.24500137162930063No Hit
TGAGGTAGTAGTTTGTACAGTT252890.24410368320594844No Hit
TTAATGCTAATCGTGATAGGGGTT246630.2380611783347822No Hit
CGCGACCTCAGATCAGACGTGG242050.233640304163865No Hit
CTTCTCACTACTGCACTTGACTAG239970.23163257091593756No Hit
GCCTGTCTGAGCGTCGCT232290.2242194020005131No Hit
ACGCCTGTCTGAGCGTCGCT224830.21701858948631178No Hit
CTCCCACTGCTTCACTTGACTAG207940.20071540941059324No Hit
CCCCCCACTGCTAAATTTGACTGG189540.18295469221738886No Hit
CAACACCAGTCGATGGGCTGT184860.1784372924095521No Hit
CCAAGTCCTTCTGATCGAGGCCC184190.17779057064219086No Hit
CCCATAAAGTAGAAAGCACT183930.17753960398619992No Hit
AACCGGGCGGAAACACCA180470.17419981694878214No Hit
TGAGGTAGTAGTTTGTGCTGTT179640.17339865416234954No Hit
CAACGGAATCCCAAAAGCAGCT178830.1726167965033009No Hit
TGTCAGTTTGTCAAATACCCCA177920.17173841320733263No Hit
ACTGGACTTGGAGTCAGAAGGCA169190.16331172510425251No Hit
GGGCTACGCCTGTCTGAGCGTCGCT168140.16229820591659683No Hit
CCCCCCACTGCTAAATTTGACTGGCT167630.1618059251683069No Hit
ACCCCCCACTGCTAAATTTGACTG166290.16051248163358442No Hit
GGAATACCGGGTGCTGTAGGCTTT166280.16050282906989247No Hit
TTCAAGTAATCCAGGATAGGCTT157450.15197961532989276No Hit
TAGCAGCACGTAAATATTGGC152810.14750082577682386No Hit
GGAATACCGGGTGCTGTAGGCTT146800.14169963499795654No Hit
AGCAGCATTGTACAGGGCTATGT144730.13970155431372108No Hit
TATTGCACTTGTCCCGGCCTGTT141940.13700848904366453No Hit
CCTCGACACAAGGGTTTGT140120.135251722451728No Hit
ACTGGACTTGGAGTCAGAAGG139420.13457604299329087No Hit
TGTAAACATCCCCGACTGGAAGC137410.13263587769120716No Hit
AACGGAATCCCAAAAGCAGCTG136870.13211463925184136No Hit
TGTAAACATCCTACACTCAGCT136410.13167062132201124No Hit
CAAAGAATTCTCCTTTTGGGCT136310.13157409568509168No Hit
CAAAGAATTCTCCTTTTGGGCTT135250.13055092393374404No Hit
TTATAAAGCAATGAGACTGATT134980.13029030471406114No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT134780.13009725344022197No Hit
TAGCAGCACGTAAATATTGGCGT133830.12918025988948587No Hit
TATTGCACTTGTCCCGGCCTGTAG133400.12876519965073163No Hit
TGAAAAGAACTTTGAAGAGAG132760.12814743557444627No Hit
TGAGGTAGTAGGTTGTGTGGTT132250.12765515482615636No Hit
CAAGTCCTTCTGATCGAGGCCC131950.1273655779153976No Hit
GTCAGGATGGCCGAGCGGTCTAAGG130100.12557985363238522No Hit
CTGCGTTCGCGCTTTCCCCTG127280.12285783067125279No Hit
TTCACAGTGGCTAAGTTCCG126410.12201805763005237No Hit
CACTAGATTGTGAGCTCCTGGA124940.1205991307673344No Hit
CAAAGTGCTGTTCGTGCAGGTAG122220.1179736334431216No Hit
ACTGGACTTGGAGTCAGAAGGCT120510.11632304505179661No Hit
AGCAGCATTGTACAGGGCTATG115630.11161259397012067No Hit
CGCGACCTCAGATCAGACGTGGC113620.10967242866803695No Hit
AGCTCGGTCTGAGGCCCCTCAGT112470.10856238384346166No Hit
ACCACAGGGTAGAACCACGGAC111730.10784809413025671No Hit
CACTAGATTGTGAGCTCCTGGAA109110.10531912244296349No Hit
ATAGGCGATAGAAATTGAAACCT105370.10170906362217086No Hit
TCGTTCGCGCTTTCCCCTG105290.10163184311263518No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)