FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12499094
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG9614337.6920215177196045No Hit
CAACGGAATCCCAAAAGCAGCTG7838296.271086528351574No Hit
AAGCTGCCAGTTGAAGAACTGT4657843.7265420997713914No Hit
CCCCCACTGCTAAATTTGACTG3659562.927860211308116No Hit
AACATTCAACGCTGTCGGTGAGT3658922.9273481741956657No Hit
TTCAAGTAATCCAGGATAGGCT3451432.7613441422234284No Hit
TAGCAGCACGTAAATATTGGCG3276932.6217340232820074No Hit
TAGCTTATCAGACTGATGTTGAC1904481.523694437372821No Hit
AACATTCAACGCTGTCGGTGAG1846841.4775790949328007No Hit
TAGCTTATCAGACTGATGTTGA1677801.342337292606968No Hit
TAGCTTATCAGACTGATGTTGACT1655701.3246560110676822No Hit
TGAGGTAGTAGGTTGTATAGTT1406641.1253935685258467No Hit
TATTGCACTTGTCCCGGCCTGT1156310.9251150523389935No Hit
TATTGCACTTGTCCCGGCCTGTAA1090830.872727255271462No Hit
TATTGCACTTGTCCCGGCCTGTAT1045450.8364206237668106No Hit
CATAAAGTAGAAAGCACTACT995900.7967777504513527No Hit
AACCGGGCGGAAACACCA928180.7425978234902466No Hit
TGTAAACATCCTTGACTGGAAGCT914720.7318290429690344No Hit
CGCCTGTCTGAGCGTCGCT874420.699586706044454No Hit
GCCTGTCTGAGCGTCGCT874030.699274683429055No Hit
TGAGGTAGTAGATTGTATAGTT813080.650511149048083No Hit
ACCGGGTGCTGTAGGCTT735010.588050621909076No Hit
AGCAGCATTGTACAGGGCTATGA699270.5594565494107013No Hit
ACTGGACTTGGAGTCAGAAGGC622260.4978440837391894No Hit
TATTGCACTTGTCCCGGCCTGTA602800.48227495528875935No Hit
CCCATAAAGTAGAAAGCACTAC568020.45444893845905954No Hit
TTCAAGTAATTCAGGATAGGTT567370.4539289007667276No Hit
TGTAAACATCCCCGACTGGAAGCT546240.4370236754759985No Hit
CAACGGAATCCCAAAAGCAGCTGT545220.4362076163280315No Hit
TCGTACGACTCTTAGCGG523430.4187743527650884No Hit
ACCGGGTGCTGTAGGCTTT476470.3812036296390763No Hit
ACCGGGTGCTGTAGGCTTA460530.36845070530712065No Hit
CCCATAAAGTAGAAAGCACT441200.352985584395157No Hit
CACCCGGCTGTGTGCACATGTGC382780.3062461967243386No Hit
CATTGCACTTGTCTCGGTCTGA373480.2988056574340508No Hit
TTCTCACTACTGCACTTGACTAG357530.2860447325222132No Hit
TGAGGTAGTAGTTTGTACAGTT350680.28056433530302277No Hit
CAACACCAGTCGATGGGCTGT348650.2789402175869707No Hit
TACGCCTGTCTGAGCGTCGCT344320.2754759664980518No Hit
CTACGCCTGTCTGAGCGTCGCT338260.2706276150895417No Hit
TTAATGCTAATCGTGATAGGGGTT334400.2675393912550782No Hit
TGTAAACATCCTACACTCTCAGCT324100.2592987939765874No Hit
AACATTCAACGCTGTCGGTGAGTT288350.23069672089833074No Hit
CCCCCACTGCTAAATTTGACTGGCTT285560.2284645591112444No Hit
TCCTGTCTGAGCGTCGCT276740.22140804765529407No Hit
GGAATACCGGGTGCTGTAGGCTTT270240.21620767073197464No Hit
CTTCTCACTACTGCACTTGACTAG255990.2048068444000821No Hit
CTCCCACTGCTTCACTTGACTAG238850.19109385048228297No Hit
ACGCCTGTCTGAGCGTCGCT237400.1899337663993886No Hit
TGAGGTAGTAGTTTGTGCTGTT236940.18956573972481525No Hit
TGTCAGTTTGTCAAATACCCCAA234330.18747758837560546No Hit
GGAATACCGGGTGCTGTAGGCTT230530.18443736802043412No Hit
CGCGACCTCAGATCAGACGTGGCG230160.184141346564799No Hit
CGCGACCTCAGATCAGACGTGG221440.17716484090766899No Hit
AGCTCGGTCTGAGGCCCCTCAGT218760.175020685499285No Hit
CCCCCCACTGCTAAATTTGACTGG216020.1728285266116088No Hit
CAACGGAATCCCAAAAGCAGCT210990.1688042349309478No Hit
CCCCCCACTGCTAAATTTGACTGGCT210910.16874023029189156No Hit
ATCCCGGACGAGCCCCCA203630.16291580813777382No Hit
TAGCTTATCAGACTGATGTTGACA199140.15932354777074242No Hit
AACGGAATCCCAAAAGCAGCTG196610.15729940106058887No Hit
TTCAAGTAATCCAGGATAGGCTT185200.14817073941519282No Hit
TATTGCACTTGTCCCGGCCTGTT184960.1479787254980241No Hit
TGAGGTAGTAGGTTGTGTGGTT179890.14392243149783496No Hit
TTCACAGTGGCTAAGTTCCG177540.14204229522555795No Hit
ACCCCCCACTGCTAAATTTGACTG175590.14048218214856212No Hit
CAAAGAATTCTCCTTTTGGGCT165200.13216957965113313No Hit
TGTCAGTTTGTCAAATACCCCA163110.1304974584557889No Hit
CACTAGATTGTGAGCTCCTGGA159700.12776926071601669No Hit
TTCAAGTAATCCAGGATAGGC158040.12644116445559975No Hit
TAGCAGCACGTAAATATTGGC156300.12504906355612655No Hit
TTATAAAGCAATGAGACTGATT150680.12055273766242577No Hit
GCGTTCGCGCTTTCCCCTG147070.117664528325013No Hit
TGTAAACATCCCCGACTGGAAGC146720.11738450802914195No Hit
AGCAGCATTGTACAGGGCTATGT144470.11558437755568524No Hit
ACCACAGGGTAGAACCACGGAC143310.11465631028936976No Hit
ACTGGACTTGGAGTCAGAAGGCA142220.11378424708222852No Hit
CAAAGAATTCTCCTTTTGGGCTT140230.11219213168570458No Hit
TGATGTAGTAGGTTGTATAGTT138280.11063201860870876No Hit
ACTGGACTTGGAGTCAGAAGG136260.10901590147253871No Hit
GGGCTACGCCTGTCTGAGCGTCGCT136050.1088478892950161No Hit
TTCACAGTGGCTAAGTTCCGC135200.10816784000504356No Hit
TATTGCACTTGTCCCGGCCTGTAG135020.10802382956716704No Hit
TGAGGTAGTAGGTTGTATAGT133340.106679732146986No Hit
ACTGGACTTGGAGTCAGAAGGCT131740.10539963936586125No Hit
TGTAAACATCCTACACTCAGCT128730.10299146482137025No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)