FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8675182
Sequences flagged as poor quality0
Sequence length18-26
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG132386615.260383009831955No Hit
CAACGGAATCCCAAAAGCAGCTG8201569.45404949429303No Hit
CCCCCACTGCTAAATTTGACTG4228884.874687355262402No Hit
TTCAAGTAATCCAGGATAGGCT2121492.445470308288633No Hit
AACATTCAACGCTGTCGGTGAGT1039411.1981420101618618No Hit
TAGCAGCACGTAAATATTGGCG738320.8510714818432628No Hit
ACCGGGTGCTGTAGGCTTA700080.8069917149864982No Hit
AAGCTGCCAGTTGAAGAACTGT695110.8012627285514009No Hit
AACATTCAACGCTGTCGGTGAG636610.733828984798244No Hit
CATAAAGTAGAAAGCACTACT537940.6200907370012525No Hit
ACCGGGTGCTGTAGGCTT531400.6125519902637201No Hit
CAACGGAATCCCAAAAGCAGCTGT522310.6020738239266912No Hit
CCCCCACTGCTAAATTTGACTGGCTT509760.5876072686428941No Hit
CCCATAAAGTAGAAAGCACTAC462900.5335911108262628No Hit
ACTGGACTTGGAGTCAGAAGGC441070.5084273736274352No Hit
ACCGGGTGCTGTAGGCTTT414890.4782493324059368No Hit
CGCCTGTCTGAGCGTCGCT410920.4736730595392696No Hit
CACCCGGCTGTGTGCACATGTGC406260.46830141431038563No Hit
CCCATAAAGTAGAAAGCACT377110.43469981379065015No Hit
AGCAGCATTGTACAGGGCTATGA359540.41444663639333446No Hit
TAGCTTATCAGACTGATGTTGA355800.41013548764740615No Hit
TCGTACGACTCTTAGCGG334310.3853636730618447No Hit
CTCCCACTGCTTCACTTGACTAG329810.38017646200390953No Hit
CAACGGAATCCCAAAAGCAGCT328860.379081384113901No Hit
CAACACCAGTCGATGGGCTGT326430.37628029014261605No Hit
CTACGCCTGTCTGAGCGTCGCT319540.3683380936561331No Hit
TAGCTTATCAGACTGATGTTGAC300900.3468515127405973No Hit
CCCCCCACTGCTAAATTTGACTGGCT289190.3333532368542816No Hit
CCCCCCACTGCTAAATTTGACTGG276560.31879446448501025No Hit
GCCTGTCTGAGCGTCGCT269060.31014911272178497No Hit
CATTGCACTTGTCTCGGTCTGA267750.30863905794714164No Hit
AACCGGGCGGAAACACCA252830.29144057150616554No Hit
CAAAGAATTCTCCTTTTGGGCT228960.2639252986277406No Hit
CACTAGATTGTGAGCTCCTGGA225170.25955651420339076No Hit
ATCCCGGACGAGCCCCCA223420.2575392654586382No Hit
TTCAAGTAATTCAGGATAGGTT210870.24307271017484128No Hit
CGCGACCTCAGATCAGACGTGGC205940.23738983228248123No Hit
CACTAGATTGTGAGCTCCTGGAA205460.2368365297696348No Hit
ACCCCCCACTGCTAAATTTGACTG191240.22044494282655974No Hit
AGCTCGGTCTGAGGCCCCTCAGT189410.21833547699633277No Hit
TTCACAGTGGCTAAGTTCCG187450.21607615840220992No Hit
CGCGACCTCAGATCAGACGTGGCG187280.21588019709557676No Hit
CCCCCCACTGCTAAATTTGACTGT184550.2127332890537628No Hit
TACGCCTGTCTGAGCGTCGCT181530.2092520940771041No Hit
TGTAAACATCCTACACTCTCAGCT171810.19804771819196418No Hit
CTTCTCACTACTGCACTTGACTAG166820.19229567748549828No Hit
TGAGGTAGTAGGTTGTATAGTT164410.1895176377855819No Hit
GGAATACCGGGTGCTGTAGGCTTT160760.18531023326081228No Hit
TAGCTTATCAGACTGATGTTGACT156720.18065327044435495No Hit
CCCATAAAGTAGAAAGCACTA152370.17563896642168428No Hit
GACCCGCGGGCGCTCTCCAGTCCT145230.16740859154309387No Hit
CGCGACCTCAGATCAGACGT136020.15679209957785323No Hit
TTCTCACTACTGCACTTGACTAG133960.15441750962688736No Hit
CAAAGAATTCTCCTTTTGGGCTT132100.15227346238960748No Hit
TATTGCACTTGTCCCGGCCTGTAA131360.15142045434896928No Hit
AACGGAATCCCAAAAGCAGCTG131060.15107464027844028No Hit
ACTGGACTTGGAGTCAGAAGGCT130650.15060202771538395No Hit
CCCCCACTGCTAAATTTGACTGGCT127640.14713235987440956No Hit
CCCCCCACTGCTAAATTTGACTGA127190.14661363876861602No Hit
TATTGCACTTGTCCCGGCCTGT124480.14348978499817064No Hit
CGCGACCTCAGATCAGACGTGG120990.13946681464434985No Hit
ACGCCTGTCTGAGCGTCGCT119870.13817577544770818No Hit
TGAGGTAGTAGATTGTATAGTT118190.13623921665274571No Hit
GGAATACCGGGTGCTGTAGGCTT115740.13341506841009215No Hit
ACTGGACTTGGAGTCAGAAGG112800.13002609051890784No Hit
AGCTACATCTGGCTACTGGGTCTC111240.12822785735215697No Hit
AGCTACATTGTCTGCTGGGTTT109590.12632587996424743No Hit
TATTGCACTTGTCCCGGCCTGTAT109540.12626824428582595No Hit
TCAGTGCACTACAGAACTTTGT109130.1257956317227696No Hit
CCCCCCACTGCTAAATTTGACTGTA108140.12465444529002388No Hit
ACTGGACTTGGAGTCAGAAGGCA107770.12422794126970478No Hit
TTCAAGTAATCCAGGATAGGCTT107090.12344409604317236No Hit
AACATTCAACGCTGTCGGTGAGTT101510.11701195433133275No Hit
ACCACAGGGTAGAACCACGGAC87750.1011506156297355No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)