FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11253689
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG127070011.291408532793113No Hit
CCCCCCACTGCTAAATTTGACTG6963896.188095299239209No Hit
TTCAAGTAATCCAGGATAGGCT4642504.125313930392069No Hit
CCCCCACTGCTAAATTTGACTG4185653.719358158911269No Hit
TAGCTTATCAGACTGATGTTGA3554373.158404324128737No Hit
TAGCAGCACGTAAATATTGGCG3469143.082669158531038No Hit
TAGCTTATCAGACTGATGTTGAC3362262.9876958568874614No Hit
TGAGGTAGTAGATTGTATAGTT3256992.894153197231592No Hit
AAGCTGCCAGTTGAAGAACTGT2211031.9647157478760964No Hit
AACATTCAACGCTGTCGGTGAG1745551.5510913799021813No Hit
TAGCTTATCAGACTGATGTTGACT1737741.544151433365539No Hit
TGAGGTAGTAGGTTGTATAGTT1699611.5102692103895887No Hit
TCGTACGACTCTTAGCGG1563131.3889934225123868No Hit
CATAAAGTAGAAAGCACTACT1328031.1800841484068025No Hit
TGAGGTAGTAGTTTGTACAGTT1071850.952443238834839No Hit
TTCAAGTAATTCAGGATAGGTT984290.8746376410437502No Hit
TATTGCACTTGTCCCGGCCTGT897410.7974362895580284No Hit
TGTAAACATCCTTGACTGGAAGCT860230.7643982342145762No Hit
TTCTCACTACTGCACTTGACTAG859870.7640783391117348No Hit
AACATTCAACGCTGTCGGTGAGT592010.5260586106475841No Hit
CATTGCACTTGTCTCGGTCTGA558290.49609510268144075No Hit
CCCCCACTGCTAAATTTGACTGGCTT548510.48740461905424964No Hit
TATTGCACTTGTCCCGGCCTGTAA537770.47786108181948156No Hit
AGCAGCATTGTACAGGGCTATGA535860.47616386057940646No Hit
CAACGGAATCCCAAAAGCAGCTGT524560.4661227087402184No Hit
TATTGCACTTGTCCCGGCCTGTAT503550.44745327509939187No Hit
TATTGCACTTGTCCCGGCCTGTA458260.40720869396692944No Hit
TGTCAGTTTGTCAAATACCCCA446130.3964300061961904No Hit
CACCCGGCTGTGTGCACATGTGC424240.3769786067484182No Hit
TTCACAGTGGCTAAGTTCCGC421230.3743039282496611No Hit
GCCTGTCTGAGCGTCGCT421130.37421506849887176No Hit
CAACGGAATCCCAAAAGCAGCT392960.34918327670153315No Hit
TAGCTTATCAGACTGATGTTGACA389130.3457799482463039No Hit
TGAGGTAGTAGATTGTATAGT371320.3299540266307342No Hit
CTTCTCACTACTGCACTTGACTAG359890.31979735711552004No Hit
TGATGTAGTAGATTGTATAGTT357230.3174336877445254No Hit
CCCATAAAGTAGAAAGCACT354370.31489229887195214No Hit
CTCCCACTGCTTCACTTGACTAG340580.3026385392381111No Hit
CCCATAAAGTAGAAAGCACTAC335600.29821332364880526No Hit
TGTAAACATCCCCGACTGGAAGCT325780.28948729612129853No Hit
TGAGAACTGAATTCCATGGGTT324780.28859869861340576No Hit
ACCGGGTGCTGTAGGCTT321640.2858085024386226No Hit
TAGCAGCACGTAAATATTGGC311750.2770202730855633No Hit
AACGGAATCCCAAAAGCAGCTG307110.272897180648941No Hit
CAACACCAGTCGATGGGCTGT301770.2681520699567937No Hit
AGAATTACTCTGAGACCT299920.2665081645671921No Hit
ACTGGACTTGGAGTCAGAAGGC298750.26546850548295764No Hit
TGAGAACTGAATTCCATAGGCTGT297280.26416226714635527No Hit
TGAGGTAGTAAGTTGTATTGTT275030.24439097259574172No Hit
TTCACAGTGGCTAAGTTCTGC234670.20852717717719052No Hit
CAAAGAATTCTCCTTTTGGGCT234500.20837611560084873No Hit
TAGCTTATCAGACTGATGTTG224110.19914358749384314No Hit
TCAGTGCACTACAGAACTTTGT218470.19413189754932803No Hit
TTCACAGTGGCTAAGTTCTG218110.19381200244648666No Hit
TTCACAGTGGCTAAGTTCCG210600.18713863516221216No Hit
TTCAAGTAATCCAGGATAGGC209230.18592125657639907No Hit
ACCGGGTGCTGTAGGCTTT202630.18005651302430697No Hit
TGTAAACATCCTACACTCTCAGCT200760.17839483568454753No Hit
TGAGGTAGTAGTTTGTGCTGTT199460.17723965892428697No Hit
TGATGTAGTAGGTTGTATAGTT186100.16536799621883988No Hit
TGTAAACATCCCCGACTGGA184280.1637507487544751No Hit
TGGCTCAGTTCAGCAGGAAC183880.163395309751318No Hit
TTATAAAGCAATGAGACTGATT177080.15735284669764732No Hit
CTCCCACTGCTTCACTTGACTAGCCT174680.15522021267870473No Hit
TGAGGTAGTAGATTGTATAGTTA169950.15101714646637204No Hit
TCAGTGCACTACAGAACTTTGTC168310.14955984655342794No Hit
CCCATAAAGTAGAAAGCACTA160620.14272653171773275No Hit
ACCCCCCACTGCTAAATTTGACTG158560.14089602085147368No Hit
TGTCAGTTTGTCAAATACCCCAA156890.13941206301329281No Hit
GTCAGGATGGCCGAGCGGTCTAAGG156530.1390921679104514No Hit
TCCCACTGCTTCACTTGACTAG155250.13795476310034868No Hit
CAAAGTGCTGTTCGTGCAGGTAG153250.1361775680845632No Hit
TTCAAGTAATCCAGGATAGGCTT152900.13586655895680075No Hit
ACCGGGTGCTGTAGGCTTA151850.13493353157351337No Hit
TGTCAGTTTGTCAAATACC151040.13421376759212025No Hit
CCCCCCACTGCTAAATTTGACTGG148800.1322233091744405No Hit
CCCCCCACTGCTAAATTTGACTGGCT145740.12950420080028868No Hit
ATCACATTGCCAGGGATTTCC144010.12796692711163424No Hit
TGTCAGTTTGTCAAATACCCC142790.12688283815200507No Hit
TATTGCACTTGTCCCGGCCTGTT139100.12360391334788084No Hit
CAAAGAATTCTCCTTTTGGGCTT130960.1163707296336339No Hit
TCGTTTCCCGGCCAATGCACCA129260.11486011387021626No Hit
TAGTGGGTTATCAGAACT128780.11443358706642773No Hit
ACCAGGATGGCCGAGTGGTTAAGGC119450.10614297231778841No Hit
CAAAGAATTCTCCTTTTGGGC117190.1041347419499508No Hit
TGAGGTAGTAGTTTGTGCTGTTA116380.10341497796855768No Hit
CACTAGATTGTGAGCTCCTGGA116190.10324614444205805No Hit
TGATGTAGTAGTTTGTACAGTT114110.10139786162564116No Hit
AATGCACCCGGGCAAGGATTCT113540.1008913610461423No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)