FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947674
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8419883
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG110847913.16501666353321No Hit
CCCCCCACTGCTAAATTTGACTG7469948.87178598562474No Hit
TTCAAGTAATCCAGGATAGGCT2918803.466556483029515No Hit
CCCCCACTGCTAAATTTGACTG2330882.7683045001931736No Hit
AACATTCAACGCTGTCGGTGAGT1663401.9755618931997039No Hit
TAGCAGCACGTAAATATTGGCG1477111.7543117879428967No Hit
AAGCTGCCAGTTGAAGAACTGT1415221.680807203615537No Hit
AACATTCAACGCTGTCGGTGAG908081.0784947961865978No Hit
CATAAAGTAGAAAGCACTACT792260.9409394406074288No Hit
CAACGGAATCCCAAAAGCAGCTGT736960.8752615683614606No Hit
CCCATAAAGTAGAAAGCACTAC735200.8731712780331984No Hit
TAGCTTATCAGACTGATGTTGAC646930.7683360920810894No Hit
TAGCTTATCAGACTGATGTTGA568300.6749499963360537No Hit
CACCCGGCTGTGTGCACATGTGC539870.6411846815448623No Hit
AGCAGCATTGTACAGGGCTATGA516240.6131201585580227No Hit
ACTGGACTTGGAGTCAGAAGGC490730.582822825447812No Hit
TCGTACGACTCTTAGCGG429630.5102564964382521No Hit
CAACGGAATCCCAAAAGCAGCT420090.49892617272710327No Hit
CATTGCACTTGTCTCGGTCTGA413710.49134887028715246No Hit
TATTGCACTTGTCCCGGCCTGTAA405960.482144466853043No Hit
TAGCTTATCAGACTGATGTTGACT405120.4811468282872814No Hit
CCCATAAAGTAGAAAGCACT401610.47697812428034925No Hit
CAACACCAGTCGATGGGCTGT394580.4686288396168925No Hit
TTCAAGTAATTCAGGATAGGTT384290.4564077671863136No Hit
AACCGGGCGGAAACACCA382790.4546262697474537No Hit
CGCCTGTCTGAGCGTCGCT373720.4438541485671475No Hit
CACTAGATTGTGAGCTCCTGGA332250.39460168270746754No Hit
TGTAAACATCCTACACTCTCAGCT320710.3808960290778387No Hit
TATTGCACTTGTCCCGGCCTGT309350.3674041551408731No Hit
AGCTCGGTCTGAGGCCCCTCAGT309220.36724975869617194No Hit
CCCCCACTGCTAAATTTGACTGGCTT308970.3669528424563619No Hit
TATTGCACTTGTCCCGGCCTGTAT305720.36309293133883214No Hit
ATCCCGGACGAGCCCCCA297560.3534015852714343No Hit
CTCCCACTGCTTCACTTGACTAG295850.351370678191134No Hit
CTACGCCTGTCTGAGCGTCGCT286090.33977906818895226No Hit
CACTAGATTGTGAGCTCCTGGAA279540.3319998627059307No Hit
TGAGGTAGTAGGTTGTATAGTT273780.3251589125407087No Hit
CCCCCCACTGCTAAATTTGACTGGCT248770.295455411910118No Hit
CAAAGAATTCTCCTTTTGGGCT246940.2932819850347089No Hit
CGCGACCTCAGATCAGACGTGGCG231940.27546701064610996No Hit
TCAGTGCACTACAGAACTTTGT211020.25062105969881054No Hit
CCCCCCACTGCTAAATTTGACTGG209170.24842387952421666No Hit
CAAAGAATTCTCCTTTTGGGCTT204090.24239054153127781No Hit
TGAGGTAGTAGATTGTATAGTT193480.2297894163137421No Hit
CTTCTCACTACTGCACTTGACTAG192380.22848298485857818No Hit
ACCGGGTGCTGTAGGCTTA191410.22733094984811547No Hit
AACGGAATCCCAAAAGCAGCTG185530.22034747988778466No Hit
TTCTCACTACTGCACTTGACTAG180790.2147179479809874No Hit
GCCTGTCTGAGCGTCGCT179690.21341151652582344No Hit
CGCGACCTCAGATCAGACGTGGC174220.206914989198781No Hit
TATTGCACTTGTCCCGGCCTGTA169480.20128545729198374No Hit
TGTAAACATCCTTGACTGGAAGCT169280.20104792430013577No Hit
TACGCCTGTCTGAGCGTCGCT165560.1966298106517632No Hit
TCGTTTCCCGGGCGGCGCACCA165420.19646353755746962No Hit
CGCGACCTCAGATCAGACGTGG159060.18890998841670364No Hit
TTCAAGTAATCCAGGATAGGCTT156900.1863446321047454No Hit
ACCACAGGGTAGAACCACGGAC155850.18509758389754347No Hit
AGCTACATCTGGCTACTGGGTCTC152320.18090512659142652No Hit
AACATTCAACGCTGTCGGTGAGTT150690.1789692327078654No Hit
CCCATAAAGTAGAAAGCACTA147330.17497867844481926No Hit
CAACGGAATCCCAAAAGCAGCTGA143750.17072683789074028No Hit
ACCCCCCACTGCTAAATTTGACTG136360.16194999384195718No Hit
TTCACAGTGGCTAAGTTCCG128950.1531493964939893No Hit
TTCACAGTGGCTAAGTTCCGC127380.1512847625079826No Hit
CCCCCCACTGCTAAATTTGACTGT124060.14734171484330602No Hit
TGTAAACATCCCCGACTGGAAGCT123910.14716356509942002No Hit
ACCGGGTGCTGTAGGCTT123420.14658160926939245No Hit
ACTGGACTTGGAGTCAGAAGGCT121490.1442894158980594No Hit
CCCCCCACTGCTAAATTTGACTGTA115760.13748409568161457No Hit
ACTGGACTTGGAGTCAGAAGGCA115510.13718717944180459No Hit
TCGTTTCCCGGCCAATGCACCA115360.13700902969791862No Hit
TGTAAACATCCTACACTCAGCT112410.13350541806816082No Hit
ACCGGGTGCTGTAGGCTTT111800.13278094244302446No Hit
ACGCCTGTCTGAGCGTCGCT111550.13248402620321445No Hit
TCAGTGCACTACAGAACTTTGTC107960.1282203089995431No Hit
CGCGACCTCAGATCAGACGT102650.12191380806597907No Hit
CAGTGCAATAGTATTGTCAAAGC102390.12160501517657668No Hit
ACTTGACTTGGAGTCAGAAGGC100220.11902778221502602No Hit
TTCAAGTAATCCAGGATAGGC100070.11884963247114004No Hit
ATCCCACCGCTGCCACCA99440.11810140354681889No Hit
ACTGGACTTGGAGTCAGAAGG99120.11772135075986209No Hit
CAAAGTGCTGTTCGTGCAGGTAG98050.11645054925347538No Hit
CCCCCCACTGCTAAATTTGACTGA97770.1161180030648882No Hit
CCCCCACTGCTAAATTTGACTGGCT96790.11495409140483306No Hit
AGCAGCATTGTACAGGGCTATGT95120.11297069092290238No Hit
ATCACATTGCCAGGGATTTCC94210.11188991580999402No Hit
CACCCGTAGAACCGACCTTGCG86770.10305368851324893No Hit
CAAAGTGCTGTTCGTGCAGGT86240.10242422608485177No Hit
TGTCAGTTTGTCAAATACCCCA84490.1003458124061819No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)