FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947675

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947675
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9600432
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5756005.99556353297435No Hit
AAGCTGCCAGTTGAAGAACTGT5074805.286012129454175No Hit
CCCCCCACTGCTAAATTTGACTG3927684.09114923161791No Hit
TTCAAGTAATCCAGGATAGGCT2927523.0493627786749595No Hit
TAGCAGCACGTAAATATTGGCG2889853.0101249610434198No Hit
AACATTCAACGCTGTCGGTGAGT2814442.9315764123947754No Hit
TAGCTTATCAGACTGATGTTGAC2246992.3405092604166144No Hit
TAGCTTATCAGACTGATGTTGACT1716221.787648722474155No Hit
CCCCCACTGCTAAATTTGACTG1365501.4223318284010553No Hit
TAGCTTATCAGACTGATGTTGA1361661.4183320083929556No Hit
AACATTCAACGCTGTCGGTGAG1336311.3919269466207354No Hit
GCCTGTCTGAGCGTCGCT1297311.3513037746634735No Hit
TATTGCACTTGTCCCGGCCTGTAA1252131.3042433923806762No Hit
TGAGGTAGTAGGTTGTATAGTT1193571.2432461372571568No Hit
TATTGCACTTGTCCCGGCCTGT1099681.1454484548195332No Hit
TGTAAACATCCTTGACTGGAAGCT1095591.1411882298629894No Hit
TATTGCACTTGTCCCGGCCTGTAT1047211.0907946642401092No Hit
CGCCTGTCTGAGCGTCGCT1018631.0610251705339926No Hit
CATAAAGTAGAAAGCACTACT794980.8280669036560021No Hit
TGAGGTAGTAGATTGTATAGTT732640.7631323257120096No Hit
AGCAGCATTGTACAGGGCTATGA631450.6577308187798215No Hit
TTCAAGTAATTCAGGATAGGTT626560.6526372979882572No Hit
TATTGCACTTGTCCCGGCCTGTA585240.6095975681094351No Hit
AACCGGGCGGAAACACCA561410.5847757684237542No Hit
TGTAAACATCCCCGACTGGAAGCT476580.4964151613177407No Hit
CCCATAAAGTAGAAAGCACTAC465320.4846865224398236No Hit
CAACGGAATCCCAAAAGCAGCTGT400220.4168770738650094No Hit
ACTGGACTTGGAGTCAGAAGGC394370.4107835980714202No Hit
TCGTACGACTCTTAGCGG382380.3982945767440465No Hit
CATTGCACTTGTCTCGGTCTGA338450.35253621920346917No Hit
TGAGGTAGTAGTTTGTACAGTT337830.35189041493132805No Hit
TGTAAACATCCTACACTCTCAGCT331160.34494281090684253No Hit
CACCCGGCTGTGTGCACATGTGC326780.3403805162101039No Hit
CAACACCAGTCGATGGGCTGT322590.3360161292741826No Hit
TACGCCTGTCTGAGCGTCGCT320970.33432870520826563No Hit
TGTCAGTTTGTCAAATACCCCAA301690.3142462755842654No Hit
CTACGCCTGTCTGAGCGTCGCT293990.3062258031721906No Hit
TTCTCACTACTGCACTTGACTAG293340.30554875030623624No Hit
ATCCCGGACGAGCCCCCA291940.3040904825949499No Hit
ACGCCTGTCTGAGCGTCGCT278380.28996611819134804No Hit
ACCGGGTGCTGTAGGCTT275500.2869662531852733No Hit
TTAATGCTAATCGTGATAGGGGTT271750.2830601789586136No Hit
TGAGGTAGTAGTTTGTGCTGTT263440.27440431847233543No Hit
AACATTCAACGCTGTCGGTGAGTT253710.26426935787889544No Hit
TAGCTTATCAGACTGATGTTGACA243040.2531552746793061No Hit
CCCATAAAGTAGAAAGCACT237660.24755136018879148No Hit
GCGTTCGCGCTTTCCCCTG222990.23227079781409837No Hit
AGCTCGGTCTGAGGCCCCTCAGT204390.21289666964986578No Hit
TGTCAGTTTGTCAAATACCCCA199720.20803230521293206No Hit
TGATGTAGTAGGTTGTATAGTT199330.2076260734933595No Hit
ACCGGGTGCTGTAGGCTTA190220.19813691717206058No Hit
ACCGGGTGCTGTAGGCTTT180350.187856129807492No Hit
CTTCTCACTACTGCACTTGACTAG175150.18243970687985708No Hit
TTCACAGTGGCTAAGTTCCGC173420.18063770463662468No Hit
CAAAGAATTCTCCTTTTGGGCT167370.17433590488428022No Hit
TGAGAACTGAATTCCATAGGCTGT165330.17221100050497726No Hit
TTCACAGTGGCTAAGTTCCG163260.17005484753186106No Hit
CTCCCACTGCTTCACTTGACTAG163240.17003401513598554No Hit
TATTGCACTTGTCCCGGCCTGTT160170.1668362423690934No Hit
TAGCAGCACGTAAATATTGGCGT157550.1641071985094004No Hit
TAGCAGCACGTAAATATTGGC156850.16337806465375723No Hit
TGAGAACTGAATTCCATGGGTT149330.15554508380456214No Hit
CCCCCACTGCTAAATTTGACTGGCTT148250.15442013442728408No Hit
CAAAGAATTCTCCTTTTGGGCTT148080.1542430590623422No Hit
AACGGAATCCCAAAAGCAGCTG146270.15235772723560773No Hit
TTCAAGTAATCCAGGATAGGCTT144920.1509515405140102No Hit
TGTAAACATCCTACACTCAGCT144030.1500244988975496No Hit
ACCACAGGGTAGAACCACGGAC143530.14950368900066163No Hit
TATTGCACTTGTCCCGGCCTGTAG142700.14863914457182759No Hit
ATCCCACCGCTGCCACCA142090.14800375649762426No Hit
TTATAAAGCAATGAGACTGATT141820.14772251915330475No Hit
CAAAGTGCTGTTCGTGCAGGTAG140830.1466913155574666No Hit
TGAGGTAGTAGGTTGTGTGGTT140500.14634758102552053No Hit
TGTAAACATCCCCGACTGGAAGC138160.1439101907080848No Hit
TTCACAGTGGCTAAGTTCTGC128300.13363981954145399No Hit
CAACGGAATCCCAAAAGCAGCT127150.13244195677861165No Hit
TGATGTAGTAGATTGTATAGTT126900.13218155183016764No Hit
TTCAAGTAATCCAGGATAGGC125870.13110868344257842No Hit
CAAAGTGCTGTTCGTGCAGGT120590.1256089309314414No Hit
AGCAGCATTGTACAGGGCTATGT120270.1252756125974331No Hit
TCGTTTCCCGGCCAATGCACCA119750.12473397030466962No Hit
GGAATACCGGGTGCTGTAGGCTTT117140.1220153426429144No Hit
AGAGGTAGTAGGTTGCATAGTT114550.1193175473770347No Hit
TGAGGTAGTAGGTTGTATAGT113630.11835925716676082No Hit
CGCGACCTCAGATCAGACGTGGCG113180.11789052825956164No Hit
GGGCTACGCCTGTCTGAGCGTCGCT112410.11708848101835416No Hit
TTCACCACCTTCTCCACCCAGC110520.11511981960811765No Hit
CCCCCCACTGCTAAATTTGACTGG110180.11476566887823382No Hit
TAGCAGCACATCATGGTTTACA106870.11131790736083544No Hit
CCAAGTCCTTCTGATCGAGGCCC105770.11017212558768189No Hit
AGCAGCATTGTACAGGGCTATG104940.10930758115884785No Hit
CCTCGACACAAGGGTTTGT104640.10899509522071507No Hit
TCGTTTCCCGGGCGGCGCACCA102450.10671394787234575No Hit
CCCCCCACTGCTAAATTTGACTGGCT101110.10531817734868597No Hit
TCAGTGCACTACAGAACTTTGT100870.10506818859817973No Hit
GGAATACCGGGTGCTGTAGGCTT100130.10429738995078555No Hit
CACTAGATTGTGAGCTCCTGGA99710.10385990963739965No Hit
TCCCCGGCACCTCCACCA97090.10113086577770668No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)