FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8718866
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG5259246.032022971794727No Hit
CAACGGAATCCCAAAAGCAGCTG3917864.493543082322861No Hit
AAGCTGCCAGTTGAAGAACTGT3076713.528796061322654No Hit
TAGCTTATCAGACTGATGTTGAC2989273.4285077898891894No Hit
AACATTCAACGCTGTCGGTGAGT2831163.24716539972056No Hit
TAGCAGCACGTAAATATTGGCG2810633.2236187595955714No Hit
TAGCTTATCAGACTGATGTTGACT2036982.3362900634096224No Hit
TTCAAGTAATCCAGGATAGGCT2012762.308511221528121No Hit
CCCCCACTGCTAAATTTGACTG1799772.064224865940135No Hit
AACATTCAACGCTGTCGGTGAG1538691.7647822549400345No Hit
CGCCTGTCTGAGCGTCGCT1478031.695208987040287No Hit
TAGCTTATCAGACTGATGTTGA1173041.3454043220758296No Hit
GCCTGTCTGAGCGTCGCT1162761.3336137979411542No Hit
TATTGCACTTGTCCCGGCCTGTAA1036251.1885146531670518No Hit
TGTAAACATCCTTGACTGGAAGCT919601.054724318506558No Hit
TGAGGTAGTAGGTTGTATAGTT847280.9717777518314881No Hit
TATTGCACTTGTCCCGGCCTGTAT803740.9218400649809275No Hit
TATTGCACTTGTCCCGGCCTGT752910.8635411990504269No Hit
AGCAGCATTGTACAGGGCTATGA618160.708991283958258No Hit
TATTGCACTTGTCCCGGCCTGTA510940.5860165760088526No Hit
ACTGGACTTGGAGTCAGAAGGC489500.561426222171553No Hit
TGAGGTAGTAGATTGTATAGTT487150.5587309175298715No Hit
TACGCCTGTCTGAGCGTCGCT467350.536021542251022No Hit
CATAAAGTAGAAAGCACTACT464990.5333147682278865No Hit
TCCTGTCTGAGCGTCGCT461510.529323423481907No Hit
TTCAAGTAATTCAGGATAGGTT404920.4644181938339229No Hit
TGTAAACATCCCCGACTGGAAGCT396350.4545889339278754No Hit
CAACACCAGTCGATGGGCTGT393390.45119399701750207No Hit
CATTGCACTTGTCTCGGTCTGA329370.37776701694922254No Hit
TTAATGCTAATCGTGATAGGGGTT319440.366377921165436No Hit
ACCGGGTGCTGTAGGCTT302840.3473387479518552No Hit
AACATTCAACGCTGTCGGTGAGTT299980.3440585048560214No Hit
TAGCTTATCAGACTGATGTTGACA296170.33968867052206103No Hit
GCGTTCGCGCTTTCCCCTG288600.33100634876141005No Hit
TGTAAACATCCTACACTCTCAGCT277180.3179083151409828No Hit
CTACGCCTGTCTGAGCGTCGCT269200.30875574874071926No Hit
ACGCCTGTCTGAGCGTCGCT259350.2974584080085644No Hit
CACCCGGCTGTGTGCACATGTGC243870.2797038055178277No Hit
CCCATAAAGTAGAAAGCACTAC242730.2783962960320757No Hit
TTCACAGTGGCTAAGTTCCG233600.2679247507646063No Hit
TGTCAGTTTGTCAAATACCCCAA229170.2628438147805001No Hit
TGAGGTAGTAGTTTGTACAGTT223640.25650124683645786No Hit
TCGTACGACTCTTAGCGG221990.25460879889655375No Hit
CAACGGAATCCCAAAAGCAGCTGT216620.24844974105577491No Hit
ACCGGGTGCTGTAGGCTTA190290.21825085968748686No Hit
TGAGGTAGTAGTTTGTGCTGTT185700.21298641360011725No Hit
ACCGGGTGCTGTAGGCTTT184500.21161008782564156No Hit
ATCCCGGACGAGCCCCCA180710.20726319225458906No Hit
TTCTCACTACTGCACTTGACTAG171820.19706691214201477No Hit
TTATAAAGCAATGAGACTGATT164620.1888089574951605No Hit
CGCGACCTCAGATCAGACGTGGCG164160.18828136594827816No Hit
CCCATAAAGTAGAAAGCACT163890.18797169264902108No Hit
CAACGGAATCCCAAAAGCAGCT161450.1851731635742538No Hit
CCTCGACACAAGGGTTTGT159460.18289075666491492No Hit
TAGCAGCACGTAAATATTGGC155920.17883059563021153No Hit
ACCACAGGGTAGAACCACGGAC150810.17296974170723578No Hit
CACCCGTAGAACCGACCTTGCG145970.16741856108351705No Hit
CCAAGTCCTTCTGATCGAGGCCC145050.16636337798975234No Hit
ACTGGACTTGGAGTCAGAAGGCA143360.1644250525240324No Hit
TGTCAGTTTGTCAAATACCCCA140600.16125950324273822No Hit
CTTCTCACTACTGCACTTGACTAG133420.15302448735879184No Hit
CAAAGAATTCTCCTTTTGGGCT133010.1525542427191793No Hit
CAAGTCCTTCTGATCGAGGCCC130970.15021448890257058No Hit
TATTGCACTTGTCCCGGCCTGTAG130620.14981306055168184No Hit
TCGTTCGCGCTTTCCCCTG130400.14956073415969462No Hit
TGTAAACATCCTACACTCAGCT128020.14683102137365112No Hit
CAAAGTGCTGTTCGTGCAGGTAG124250.14250706456550657No Hit
TAGCAGCACGTAAATATTGGCGT124070.1423006156993352No Hit
AACGGAATCCCAAAAGCAGCTG119490.13704763899341957No Hit
CGCGACCTCAGATCAGACGTGG119420.1369673533232418No Hit
TATTGCACTTGTCCCGGCCTGTT119020.13650857806508324No Hit
TCAGTGCACTACAGAACTTTGT117380.13462759950663308No Hit
CTGCGTTCGCGCTTTCCCCTG113800.13052156094611386No Hit
TCGTTTCCCGGCCAATGCACCA113530.13021188764685682No Hit
TGAGGTAGTAGGTTGTGTGGTT111380.12774597063425452No Hit
CAAAGAATTCTCCTTTTGGGCTT110530.12677107321066755No Hit
CAACACCAGTCGATGGGCTGTC108390.12431662557951917No Hit
AGCAGCATTGTACAGGGCTATG108040.12391519722863041No Hit
AACCGGGCGGAAACACCA107190.12294029980504345No Hit
TGAGAACTGAATTCCATAGGCTGT106730.1224127082581611No Hit
AGCAGCATTGTACAGGGCTATGT106100.12169013722656133No Hit
CCCCCACTGCTAAATTTGACTGGCTT104660.12003854629719048No Hit
ATAGGCGATAGAAATTGAAACCT102880.11799699639838483No Hit
CACTAGATTGTGAGCTCCTGGA102030.11702209897479787No Hit
ACTGGACTTGGAGTCAGAAGG101970.11695328268607409No Hit
TTCACAGTGGCTAAGTTCCGC101900.11687299701589632No Hit
TCAGTGCACTACAGAACTTTGTC101770.11672389505699479No Hit
TTCAAGTAATCCAGGATAGGCTT101550.11647156866500757No Hit
ACTGGACTTGGAGTCAGAAGGCT98110.11252610144484386No Hit
TTCACAGTGGCTAAGTTCTG97960.1123540607230344No Hit
CTCCCACTGCTTCACTTGACTAG97620.11196410175359961No Hit
TGTAAACATCCCCGACTGGAAGC97050.11131034701072363No Hit
CCCCCCACTGCTAAATTTGACTGG88370.10135492390868261No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)