FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8009525
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG122251515.263264675495739No Hit
TCGTACGACTCTTAGCGG5334336.659982957790881No Hit
CCCCCCACTGCTAAATTTGACTG3808174.754551611986978No Hit
TTCAAGTAATCCAGGATAGGCT2486723.1047034624400323No Hit
CCCCCACTGCTAAATTTGACTG1879992.3471928734850067No Hit
TAGCAGCACGTAAATATTGGCG1744802.178406334957441No Hit
TAGCTTATCAGACTGATGTTGAC1514191.89048663934503No Hit
AAGCTGCCAGTTGAAGAACTGT1449971.810307103105365No Hit
TGAGGTAGTAGATTGTATAGTT1316441.6435930969689212No Hit
AACATTCAACGCTGTCGGTGAG1172111.4633951451552993No Hit
TAGCTTATCAGACTGATGTTGA1148761.434242355195845No Hit
TATTGCACTTGTCCCGGCCTGT950451.186649645266105No Hit
TGAGGTAGTAGGTTGTATAGTT870061.086281645915332No Hit
TAGCTTATCAGACTGATGTTGACT786420.9818559777265194No Hit
TATTGCACTTGTCCCGGCCTGTAA752320.9392816677643181No Hit
GCCTGTCTGAGCGTCGCT601160.7505563688233696No Hit
CATAAAGTAGAAAGCACTACT577970.7216033410220956No Hit
TATTGCACTTGTCCCGGCCTGTAT547370.6833988282700909No Hit
TTCAAGTAATTCAGGATAGGTT503730.6289136996263823No Hit
CATTGCACTTGTCTCGGTCTGA470960.5879999126040558No Hit
CAACGGAATCCCAAAAGCAGCTGT465430.5810956330119452No Hit
CACCCGGCTGTGTGCACATGTGC449340.5610070509799271No Hit
TATTGCACTTGTCCCGGCCTGTA448980.5605575861240211No Hit
TGAGGTAGTAGTTTGTACAGTT432280.5397074108639401No Hit
CAACGGAATCCCAAAAGCAGCT413710.5165225153801255No Hit
TGTAAACATCCTTGACTGGAAGCT404200.504649152103277No Hit
AACGGAATCCCAAAAGCAGCTG349900.4368548696707982No Hit
AACATTCAACGCTGTCGGTGAGT336830.4205367983744354No Hit
AGCAGCATTGTACAGGGCTATGA335290.4186140876019489No Hit
TCGTTTCCCGGCCAATGCACCA326430.4075522580927084No Hit
ACCGGGTGCTGTAGGCTT323330.4036818662779628No Hit
CCCATAAAGTAGAAAGCACTAC301950.3769886478911047No Hit
CCCATAAAGTAGAAAGCACT297990.3720445344761394No Hit
CAACACCAGTCGATGGGCTGT294720.3679618953683271No Hit
TTCTCACTACTGCACTTGACTAG287220.35859804420362007No Hit
TTCACAGTGGCTAAGTTCCGC261320.32626154484816516No Hit
CCCCCACTGCTAAATTTGACTGGCTT236790.2956355089721301No Hit
ACTGGACTTGGAGTCAGAAGGC210540.2628620298956555No Hit
TGTAAACATCCCCGACTGGAAGCT202250.2525118530749327No Hit
ACCGGGTGCTGTAGGCTTT197350.2463941369806574No Hit
CTCCCACTGCTTCACTTGACTAG196910.2458447910456613No Hit
TAGCTTATCAGACTGATGTTGACA177710.2218733320640113No Hit
TCAGTGCACTACAGAACTTTGT176630.22052493749629345No Hit
TAGCAGCACGTAAATATTGGC174000.21724134702120287No Hit
GGAATACCGGGTGCTGTAGGCTTT159380.19898807981746733No Hit
TGAGGTAGTAGATTGTATAGT159370.19897559468258105No Hit
CCCATAAAGTAGAAAGCACTA158350.19770211092418088No Hit
TGATGTAGTAGATTGTATAGTT155610.19428118396534125No Hit
GGAATACCGGGTGCTGTAGGCTT154430.1928079380487607No Hit
AACCGGGCGGAAACACCA150380.1877514584198189No Hit
TGAGAACTGAATTCCATGGGTT146880.1833816612096223No Hit
TGTCAGTTTGTCAAATACCCCA146850.18334420580496347No Hit
CTCGTACGACTCTTAGCGG146330.1826949787908771No Hit
ACCGGGTGCTGTAGGCTTA139680.17439236409150355No Hit
TGAGAACTGAATTCCATAGGCTGT137930.17220746548640525No Hit
CTTCTCACTACTGCACTTGACTAG136280.1701474182301697No Hit
TCAGTGCACTACAGAACTTTGTC135550.16923600338347156No Hit
TGAGGTAGTAGTTTGTGCTGTT134040.16735074801564387No Hit
TTCACAGTGGCTAAGTTCTGC133450.1666141250573536No Hit
TGGCTCAGTTCAGCAGGAAC129340.16148273461909415No Hit
TGTAAACATCCCCGACTGGA127870.15964741979081157No Hit
CACTAGATTGTGAGCTCCTGGA126950.15849878738127418No Hit
AGAATTACTCTGAGACCT125810.1570754820042387No Hit
TGTAAACATCCTACACTCTCAGCT121040.15112007266348504No Hit
AATGCACCCGGGCAAGGATTCT118950.14851067947225335No Hit
TTCACAGTGGCTAAGTTCTG117840.1471248294998767No Hit
TCGTTTCCCGGCCAACGCACCA117350.1465130578904492No Hit
CACCCGTAGAACCGACCTTGCG116590.1455641876390922No Hit
TTCAAGTAATCCAGGATAGGC116550.1455142470995471No Hit
AGCTCGGTCTGAGGCCCCTCAGT110340.13776097833516968No Hit
CAAAGAATTCTCCTTTTGGGCT109810.13709926618619706No Hit
TTCACAGTGGCTAAGTTCCG105700.1319678757479376No Hit
TATTGCACTTGTCCCGGCCTGTT103540.129271086612502No Hit
CAAAGTGCTGTTCGTGCAGGTAG101650.12691139611899582No Hit
CACTAGATTGTGAGCTCCTGGAA101090.12621222856536435No Hit
TGATGTAGTAGGTTGTATAGTT100910.1259874961374114No Hit
TGAGGTAGTAAGTTGTATTGTT99180.12382756780208565No Hit
CTCCCACTGCTTCACTTGACTAGCCT98190.12259153944834432No Hit
ACCCCCCACTGCTAAATTTGACTG87800.10961948430150352No Hit
TATTGCACTTGTCCCGGCCTGTAG86990.10860818837571518No Hit
TCGGACGACTCTTAGCGG85620.10689772489629536No Hit
CAACGGAATCCCAAAAGCAGCTGA83250.10393874792824792No Hit
CCCCCCACTGCTAAATTTGACTGGCT82520.10302733308154978No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)