FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8411550
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG167359919.89644001402833No Hit
CAACGGAATCCCAAAAGCAGCTG91343210.859258995072253No Hit
CCCCCACTGCTAAATTTGACTG5194316.175211465187748No Hit
TTCAAGTAATCCAGGATAGGCT2059792.448763902015681No Hit
AACATTCAACGCTGTCGGTGAGT1881282.2365437998941933No Hit
TAGCAGCACGTAAATATTGGCG1281721.523761970148189No Hit
AAGCTGCCAGTTGAAGAACTGT1163041.3826702569680973No Hit
AACATTCAACGCTGTCGGTGAG1061191.261586746794586No Hit
CATAAAGTAGAAAGCACTACT743930.8844148819183146No Hit
CCCATAAAGTAGAAAGCACTAC561930.6680457228453733No Hit
CAACGGAATCCCAAAAGCAGCTGT544900.6477997515321195No Hit
CACCCGGCTGTGTGCACATGTGC453480.5391158585516344No Hit
ACTGGACTTGGAGTCAGAAGGC429110.5101437903834608No Hit
CGCCTGTCTGAGCGTCGCT414720.49303636071829804No Hit
AGCAGCATTGTACAGGGCTATGA412690.4906230124055614No Hit
TAGCTTATCAGACTGATGTTGA373070.4435211108535288No Hit
TCGTACGACTCTTAGCGG357050.42447586948897653No Hit
CAACGGAATCCCAAAAGCAGCT355800.42298981757226667No Hit
TAGCTTATCAGACTGATGTTGAC355690.4228590450035962No Hit
CCCATAAAGTAGAAAGCACT354140.4210163406268761No Hit
CATTGCACTTGTCTCGGTCTGA349210.41515535186737285No Hit
TATTGCACTTGTCCCGGCCTGTAA315300.3748417354708704No Hit
TATTGCACTTGTCCCGGCCTGT314560.37396199273617825No Hit
TTCAAGTAATTCAGGATAGGTT298300.35463142940361764No Hit
CCCCCCACTGCTAAATTTGACTGG288650.34315910860661825No Hit
ATCCCGGACGAGCCCCCA285520.33943803460717703No Hit
CAACACCAGTCGATGGGCTGT283400.3369176905564373No Hit
CTCCCACTGCTTCACTTGACTAG281310.3344330117516986No Hit
GCCTGTCTGAGCGTCGCT268120.31875219192657717No Hit
TATTGCACTTGTCCCGGCCTGTAT262260.3117855805410418No Hit
AGCTCGGTCTGAGGCCCCTCAGT250250.2975075937252944No Hit
AACCGGGCGGAAACACCA243800.28983956583507203No Hit
CTACGCCTGTCTGAGCGTCGCT238990.28412123805957284No Hit
CACTAGATTGTGAGCTCCTGGA234290.27853368285274416No Hit
TTCTCACTACTGCACTTGACTAG228890.27211393857255795No Hit
TGAGGTAGTAGGTTGTATAGTT226780.26960548293715186No Hit
CTTCTCACTACTGCACTTGACTAG215980.25676599437677955No Hit
TAGCTTATCAGACTGATGTTGACT213730.25409110092670195No Hit
CACTAGATTGTGAGCTCCTGGAA193250.22974362632332923No Hit
CCCCCCACTGCTAAATTTGACTGT189270.22501203702052536No Hit
TGTAAACATCCTACACTCTCAGCT188280.2238350839024912No Hit
CCCCCCACTGCTAAATTTGACTGA186580.22181405329576592No Hit
CCCCCACTGCTAAATTTGACTGGCTT184970.2199000184270438No Hit
ACCCCCCACTGCTAAATTTGACTG183920.21865173481700756No Hit
TGAGGTAGTAGATTGTATAGTT180760.21489499557156527No Hit
CCCCCCACTGCTAAATTTGACTGGCT176880.21028229042209817No Hit
TACGCCTGTCTGAGCGTCGCT172300.20483739619927363No Hit
GACCCGCGGGCGCTCTCCAGTCCT168830.2007121160784873No Hit
TATTGCACTTGTCCCGGCCTGTA166930.19845331716508846No Hit
CGCGACCTCAGATCAGACGTGGCG163080.1938762772616224No Hit
AACGGAATCCCAAAAGCAGCTG161840.19240211376024632No Hit
CAAAGAATTCTCCTTTTGGGCT158560.1885027135307999No Hit
ACGCCTGTCTGAGCGTCGCT145890.1734400913030298No Hit
CCCCCCACTGCTAAATTTGACTGTA134630.16005373563730824No Hit
AACATTCAACGCTGTCGGTGAGTT133060.15818725442992077No Hit
CAAAGAATTCTCCTTTTGGGCTT133030.15815158918391972No Hit
GGAATACCGGGTGCTGTAGGCTTT130710.1553934768265064No Hit
CGCGACCTCAGATCAGACGT127500.15157729550439575No Hit
CGCGACCTCAGATCAGACGTGGC119670.1422686662981258No Hit
TGTAAACATCCTTGACTGGAAGCT117780.14002175580006063No Hit
ACCACAGGGTAGAACCACGGAC116540.13854759229868455No Hit
CCCATAAAGTAGAAAGCACTA116420.1384049313146804No Hit
TTCACAGTGGCTAAGTTCCGC110780.13169986506648595No Hit
TTCACAGTGGCTAAGTTCCG110110.13090334123912953No Hit
ACTGGACTTGGAGTCAGAAGGCT109980.1307487918397917No Hit
TTCAAGTAATCCAGGATAGGCTT109430.1300949289964394No Hit
TCGTTTCCCGGGCGGCGCACCA100980.12004921803948143No Hit
TCCTGTCTGAGCGTCGCT100160.11907436798211982No Hit
CAACGGAATCCCAAAAGCAGCTGA97640.11607848731803294No Hit
CGCGACCTCAGATCAGACGTGG97120.11546028972068169No Hit
TGTAAACATCCCCGACTGGAAGCT97010.11532951715201123No Hit
GGAATACCGGGTGCTGTAGGCTT96100.11424767135664651No Hit
TGTAAACATCCTACACTCAGCT91870.10921887167050068No Hit
ACCGGGTGCTGTAGGCTTA91490.1087671118878209No Hit
ACTGGACTTGGAGTCAGAAGGCA91370.10862445090381678No Hit
ACTTGACTTGGAGTCAGAAGGC90320.10737616729378059No Hit
ACTGGACTTGGAGTCAGAAGG87940.10454672444436519No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)