FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10236598
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG129117612.613331108635897No Hit
CAACGGAATCCCAAAAGCAGCTG6490436.340417001820331No Hit
CCCCCACTGCTAAATTTGACTG4053823.960124252217387No Hit
AACATTCAACGCTGTCGGTGAGT3438753.359270335711142No Hit
AAGCTGCCAGTTGAAGAACTGT3166603.0934105256453366No Hit
TAGCAGCACGTAAATATTGGCG2955632.887316665165517No Hit
TTCAAGTAATCCAGGATAGGCT2794732.730135539170338No Hit
AACATTCAACGCTGTCGGTGAG1661801.6233908960769976No Hit
TGAGGTAGTAGGTTGTATAGTT1299271.269240034628692No Hit
TAGCTTATCAGACTGATGTTGAC1163781.1368816085187676No Hit
TATTGCACTTGTCCCGGCCTGTAA1044541.0203975969360133No Hit
GCCTGTCTGAGCGTCGCT1022210.998583709158062No Hit
TAGCTTATCAGACTGATGTTGACT1017010.9935038965093677No Hit
TATTGCACTTGTCCCGGCCTGT958890.9367272212897293No Hit
TATTGCACTTGTCCCGGCCTGTAT919640.8983844046625648No Hit
CGCCTGTCTGAGCGTCGCT917260.8960594134887392No Hit
TAGCTTATCAGACTGATGTTGA895090.8744018276384401No Hit
CATAAAGTAGAAAGCACTACT853340.8336167933917109No Hit
TGTAAACATCCTTGACTGGAAGCT784840.7667000306156401No Hit
TGAGGTAGTAGATTGTATAGTT732360.7154329983457395No Hit
GGAATACCGGGTGCTGTAGGCTTT668240.652795000839146No Hit
AGCAGCATTGTACAGGGCTATGA652470.6373894920949323No Hit
ACTGGACTTGGAGTCAGAAGGC599430.5855754030782492No Hit
TATTGCACTTGTCCCGGCCTGTA524470.5123479499732235No Hit
GGAATACCGGGTGCTGTAGGCTT497140.48564962695614305No Hit
TGTAAACATCCCCGACTGGAAGCT485800.4745717278337979No Hit
TTCAAGTAATTCAGGATAGGTT482310.47116239203688565No Hit
CCCATAAAGTAGAAAGCACTAC477010.4659848906834087No Hit
CAACGGAATCCCAAAAGCAGCTGT443950.4336890048822861No Hit
TTCTCACTACTGCACTTGACTAG399710.3904715218864705No Hit
AACCGGGCGGAAACACCA394120.385010723289124No Hit
TGAGGTAGTAGTTTGTACAGTT336500.3287224915933985No Hit
TACGCCTGTCTGAGCGTCGCT335350.3275990714883988No Hit
TCGTACGACTCTTAGCGG333820.32610443430522523No Hit
CGCGACCTCAGATCAGACGTGGCG313700.3064494669029691No Hit
TCCTGTCTGAGCGTCGCT302610.2956157895425805No Hit
CTACGCCTGTCTGAGCGTCGCT297090.29022337303858176No Hit
TGTAAACATCCTACACTCTCAGCT282250.27572636924884614No Hit
CATTGCACTTGTCTCGGTCTGA278590.27215096265380356No Hit
CCCATAAAGTAGAAAGCACT278550.2721118871718905No Hit
CACCCGGCTGTGTGCACATGTGC264010.25790794949650264No Hit
AACATTCAACGCTGTCGGTGAGTT255680.2497704803881133No Hit
ACGCCTGTCTGAGCGTCGCT247520.24179908207785436No Hit
CCCCCCACTGCTAAATTTGACTGG247500.24177954433689786No Hit
TGTCAGTTTGTCAAATACCCCAA238580.2330657118702913No Hit
CTTCTCACTACTGCACTTGACTAG238270.23276287688546526No Hit
TTAATGCTAATCGTGATAGGGGTT229820.22450818133133685No Hit
TGAGGTAGTAGTTTGTGCTGTT220170.21508122131981738No Hit
TGAATACCGGGTGCTGTAGGCTTT219880.21479792407594786No Hit
CGCGACCTCAGATCAGACGTGG205420.2006721373643861No Hit
CAACACCAGTCGATGGGCTGT195700.19117679525951883No Hit
CCCCCACTGCTAAATTTGACTGGCTT193330.18886157295617156No Hit
AACGGAATCCCAAAAGCAGCTG182480.17826234848726108No Hit
ATCCCGGACGAGCCCCCA181000.17681655565647883No Hit
TGAGGTAGTAGGTTGTGTGGTT175700.17163905430300186No Hit
CAACGGAATCCCAAAAGCAGCT174040.17001742180361093No Hit
GACCCGCGGGCGCTCTCCAGTCCT172280.16829810059943742No Hit
CCCCCCACTGCTAAATTTGACTGGCT170050.16611964248278577No Hit
ACCCCCCACTGCTAAATTTGACTG167580.16370673147465595No Hit
AGCTCGGTCTGAGGCCCCTCAGT166930.16307175489356912No Hit
CTCCCACTGCTTCACTTGACTAG163410.15963311248522213No Hit
TGAATACCGGGTGCTGTAGGCTT162290.1585389989916572No Hit
TGTAAACATCCCCGACTGGAAGC162150.15840223480496157No Hit
TTATAAAGCAATGAGACTGATT156400.15278513427996293No Hit
TATTGCACTTGTCCCGGCCTGTT152350.14882874173626826No Hit
ACTGGACTTGGAGTCAGAAGGCA149040.1455952456079647No Hit
TGTCAGTTTGTCAAATACCCCA144170.14083780568505277No Hit
GCGTTCGCGCTTTCCCCTG143300.1399879139534443No Hit
TAGCTTATCAGACTGATGTTGACA140860.13760430955674924No Hit
TTCAAGTAATCCAGGATAGGCTT137870.13468341728374994No Hit
CAAAGAATTCTCCTTTTGGGCT136710.13355022830827196No Hit
TTCACAGTGGCTAAGTTCCG136080.13293478946814166No Hit
CCCCCCACTGCTAAATTTGACTGTA135670.13253426577853308No Hit
TAGCAGCACGTAAATATTGGC132100.1290467790177948No Hit
CGCGACCTCAGATCAGACGTGGC130370.12735676442505606No Hit
CACTAGATTGTGAGCTCCTGGA129880.12687808977162138No Hit
ACCACAGGGTAGAACCACGGAC129710.126712018973491No Hit
TTCAAGTAATCCAGGATAGGC126020.1231073057670136No Hit
CAAAGAATTCTCCTTTTGGGCTT124950.12206203662583995No Hit
CGCGACCTCAGATCAGACGT123130.1202841021987969No Hit
TATTGCACTTGTCCCGGCCTGTAG121530.11872108292227555No Hit
ACTGGACTTGGAGTCAGAAGGCT121170.11836940358505824No Hit
TGAGGTAGTAGGTTGTATAGT120160.11738274766675413No Hit
TGTAAACATCCTACACTCAGCT117250.11454000635758091No Hit
ACTGGACTTGGAGTCAGAAGG114330.11168749617792943No Hit
AGAGGTAGTAGGTTGCATAGTT113670.11104275072636437No Hit
AGCAGCATTGTACAGGGCTATGT113330.11071060913010358No Hit
CGCGACCTCAGATCAGACGTG112570.10996817497375592No Hit
CCCCCCACTGCTAAATTTGACTGT112410.10981187304610379No Hit
CAGGTCCCTGTTCGGGCGCCA112330.10973372208227773No Hit
ACCGGGTGCTGTAGGCTT110970.10840515569723458No Hit
GGAATACCGGGTGCTGTAGGCTTA109950.10740873090845221No Hit
AGCAGCATTGTACAGGGCTATG108980.10646115047206113No Hit
TTCACAGTGGCTAAGTTCTGC108390.10588478711384389No Hit
GGAATACCAGGTGCTGTAGGCTTT107990.10549403229471353No Hit
TGAGAACTGAATTCCATGGGTT107920.10542565020136574No Hit
CAAAGTGCTGTTCGTGCAGGT107350.10486882458410499No Hit
TAGCAGCACGTAAATATTGGCGT107060.1045855273402355No Hit
CCCCCCACTGCTAAATTTGACTGA103640.1012445736366711No Hit
AACATTCAACGCTGTCGGTGA103590.10119572928427979No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)