FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8634297
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG106432612.326724457127199No Hit
CAACGGAATCCCAAAAGCAGCTG4342125.028921289133325No Hit
CCCCCACTGCTAAATTTGACTG3617654.189860506304104No Hit
AAGCTGCCAGTTGAAGAACTGT3100603.591027735089493No Hit
AACATTCAACGCTGTCGGTGAGT2653543.073255413845505No Hit
TAGCAGCACGTAAATATTGGCG2194032.5410638526796103No Hit
TTCAAGTAATCCAGGATAGGCT2002622.3193781728842544No Hit
CGCCTGTCTGAGCGTCGCT1896032.195928632058869No Hit
AACATTCAACGCTGTCGGTGAG1429371.6554561419418394No Hit
TAGCTTATCAGACTGATGTTGAC1366651.5828156015481054No Hit
TGAGGTAGTAGGTTGTATAGTT995111.152508420778206No Hit
GCCTGTCTGAGCGTCGCT937861.0862030805750602No Hit
TAGCTTATCAGACTGATGTTGACT906411.049778574908878No Hit
TATTGCACTTGTCCCGGCCTGT852120.9869014234743141No Hit
TATTGCACTTGTCCCGGCCTGTAA796600.9225997206257788No Hit
TAGCTTATCAGACTGATGTTGA755150.87459349614682No Hit
TGTAAACATCCTTGACTGGAAGCT733440.8494495846042822No Hit
TATTGCACTTGTCCCGGCCTGTAT699790.8104771007992891No Hit
CATAAAGTAGAAAGCACTACT628250.7276214844126858No Hit
TGAGGTAGTAGATTGTATAGTT594560.6886026737324417No Hit
AGCAGCATTGTACAGGGCTATGA578370.6698518709745565No Hit
TACGCCTGTCTGAGCGTCGCT538470.6236408129115781No Hit
ACTGGACTTGGAGTCAGAAGGC493230.5712451170025771No Hit
TATTGCACTTGTCCCGGCCTGTA486150.5630452600831313No Hit
TCGTACGACTCTTAGCGG405880.470078803172974No Hit
TTCAAGTAATTCAGGATAGGTT400150.463442478293253No Hit
TCCTGTCTGAGCGTCGCT399220.4623653784436649No Hit
ACGCCTGTCTGAGCGTCGCT390340.4520808121379193No Hit
TGTAAACATCCCCGACTGGAAGCT348450.40356499203119833No Hit
GCGTTCGCGCTTTCCCCTG327160.37890751267879713No Hit
CTACGCCTGTCTGAGCGTCGCT324970.37637111625879904No Hit
AACCGGGCGGAAACACCA304150.3522579776905983No Hit
CATTGCACTTGTCTCGGTCTGA277040.3208599379891611No Hit
CCCATAAAGTAGAAAGCACTAC264360.30617431853456045No Hit
TGAGGTAGTAGTTTGTACAGTT260090.3012289246015049No Hit
CAACACCAGTCGATGGGCTGT252550.2924963086166714No Hit
CAACGGAATCCCAAAAGCAGCTGT239330.2771852763461808No Hit
TTCTCACTACTGCACTTGACTAG230390.2668312197275586No Hit
AACATTCAACGCTGTCGGTGAGTT222180.25732262858226906No Hit
CACCCGGCTGTGTGCACATGTGC204610.23697354862822068No Hit
ATCCCGGACGAGCCCCCA188950.21883657696741263No Hit
TGAGGTAGTAGTTTGTGCTGTT188640.21847754368421657No Hit
CCCATAAAGTAGAAAGCACT186410.21589482038896737No Hit
TGTCAGTTTGTCAAATACCCCAA181030.20966385566769363No Hit
TGTAAACATCCTACACTCTCAGCT177880.206015614241669No Hit
TTATAAAGCAATGAGACTGATT174900.2025642620354616No Hit
TTAATGCTAATCGTGATAGGGGTT172060.19927505389263309No Hit
ACCGGGTGCTGTAGGCTT165470.1916427011950133No Hit
TACCTGGTTGATCCTGCC158560.18363973349538473No Hit
CCTCGACACAAGGGTTTGT152230.1763085054868972No Hit
TCGTTCGCGCTTTCCCCTG147270.17056397295576003No Hit
CTTCTCACTACTGCACTTGACTAG146390.16954478170023568No Hit
CAACGGAATCCCAAAAGCAGCT143220.16587337683658554No Hit
AACGGAATCCCAAAAGCAGCTG136240.15778933710526752No Hit
CCCCCCACTGCTAAATTTGACTGG136100.15762719304188863No Hit
ACCACAGGGTAGAACCACGGAC135980.1574882124161353No Hit
TAGCTTATCAGACTGATGTTGACA130900.15160469925924483No Hit
TGAGGTAGTAGGTTGTGTGGTT130790.1514773003523043No Hit
TAGCAGCACGTAAATATTGGC129260.1497052973739495No Hit
TTCACAGTGGCTAAGTTCCG125380.1452115904745922No Hit
CTCGTACGACTCTTAGCGG124250.14390285624874846No Hit
CGCGACCTCAGATCAGACGTGGCG121010.14015037935340885No Hit
TATTGCACTTGTCCCGGCCTGTT118270.1369769883987081No Hit
ACTGGACTTGGAGTCAGAAGGCA117790.1364210658956948No Hit
TGTCAGTTTGTCAAATACCCCA116620.13506600479459993No Hit
TTCAAGTAATCCAGGATAGGCTT116490.13491544245003384No Hit
AGCTCGGTCTGAGGCCCCTCAGT116080.13444059197871No Hit
ACCGGGTGCTGTAGGCTTT113490.1314409268062009No Hit
CCAAGTCCTTCTGATCGAGGCCC113210.13111663867944318No Hit
ACTGGACTTGGAGTCAGAAGG112580.13038699039423823No Hit
CTCGACACAAGGGTTTGT110830.12836018960200235No Hit
TAGCAGCACGTAAATATTGGCGT109860.127236762877163No Hit
CTCCCACTGCTTCACTTGACTAG108790.12599751896419592No Hit
CGCGACCTCAGATCAGACGTGG108170.12527945239780378No Hit
TGTAAACATCCTACACTCAGCT105510.12219871519360521No Hit
CAAGTCCTTCTGATCGAGGCCC105470.12215238831835412No Hit
AGCAGCATTGTACAGGGCTATG103770.1201834961201821No Hit
TGAGGTAGTAGGTTGTATAGT103380.11973180908648381No Hit
AGCAGCATTGTACAGGGCTATGT103290.11962757361716884No Hit
ACCGGGTGCTGTAGGCTTA101380.1174154653239285No Hit
TTCACAGTGGCTAAGTTCCGC98910.11455478077717271No Hit
CTGCGTTCGCGCTTTCCCCTG97700.11315339280082676No Hit
TGTAAACATCCCCGACTGGAAGC96930.11226160045224297No Hit
TATTGCACTTGTCCCGGCCTGTAG95080.1101189824718793No Hit
CAAAGTGCTGTTCGTGCAGGTAG92760.10743202370731514No Hit
ACCCCCCACTGCTAAATTTGACTG90550.10487246384969152No Hit
ACTGGACTTGGAGTCAGAAGGCT88290.10225499539800403No Hit
CAAAGTGCTGTTCGTGCAGGT87850.10174539977024187No Hit
TTCACAGTGGCTAAGTTCTGC87430.10125896758010525No Hit
GACCCGCGGGCGCTCTCCAGTCCT87330.10114315039197748No Hit
TTCAAGTAATCCAGGATAGGC87140.10092309773453473No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)