FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7665118
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG165032521.53032738700174No Hit
CCCCCACTGCTAAATTTGACTG4375105.707805150553455No Hit
CAACGGAATCCCAAAAGCAGCTG3517494.588957404178252No Hit
TAGCTTATCAGACTGATGTTGAC1749822.282835045722714No Hit
AACATTCAACGCTGTCGGTGAGT1615212.107221310878711No Hit
TAGCAGCACGTAAATATTGGCG1591662.0764977134076736No Hit
TTCAAGTAATCCAGGATAGGCT1527261.9924807419794452No Hit
AAGCTGCCAGTTGAAGAACTGT1505481.9640663066113266No Hit
TAGCTTATCAGACTGATGTTGACT1291531.6849447066568317No Hit
AACATTCAACGCTGTCGGTGAG1105271.4419477951937596No Hit
TATTGCACTTGTCCCGGCCTGTAA984031.2837767142006165No Hit
TATTGCACTTGTCCCGGCCTGTAT801131.045163296899017No Hit
TGTAAACATCCTTGACTGGAAGCT772301.0075513514599514No Hit
TGAGGTAGTAGGTTGTATAGTT610570.7965565565983459No Hit
AGCAGCATTGTACAGGGCTATGA567850.7408235594024776No Hit
TAGCTTATCAGACTGATGTTGA559060.729356025569339No Hit
TATTGCACTTGTCCCGGCCTGTA470080.6132717069717648No Hit
ACTGGACTTGGAGTCAGAAGGC441480.5759598221449429No Hit
CGCCTGTCTGAGCGTCGCT425250.5547859798114002No Hit
TTAATGCTAATCGTGATAGGGGTT424340.5535987834760013No Hit
TATTGCACTTGTCCCGGCCTGT411090.5363126829880506No Hit
TGTAAACATCCCCGACTGGAAGCT402100.5245842268833957No Hit
TGAGGTAGTAGATTGTATAGTT380580.4965089904682485No Hit
TTCAAGTAATTCAGGATAGGTT360470.47027325606729076No Hit
TAGCTTATCAGACTGATGTTGACA274290.35784184927094403No Hit
AACCGGGCGGAAACACCA269860.35206242095685936No Hit
CATAAAGTAGAAAGCACTACT242330.3161464702826493No Hit
ACCCCCCACTGCTAAATTTGACTG217750.2840791231133037No Hit
CCCCCCACTGCTAAATTTGACTGG214950.28042621131207635No Hit
CCCCCACTGCTAAATTTGACTGGCTT211980.2765515155800602No Hit
TGTAAACATCCTACACTCTCAGCT206810.2698066748613655No Hit
CAACGGAATCCCAAAAGCAGCTGT197820.2580782187567106No Hit
CAAAGAATTCTCCTTTTGGGCT186890.2438188166183482No Hit
AACATTCAACGCTGTCGGTGAGTT185420.24190103792270387No Hit
TACGCCTGTCTGAGCGTCGCT182970.23870474009662995No Hit
TTCTCACTACTGCACTTGACTAG178130.23239042112593702No Hit
GCCTGTCTGAGCGTCGCT166200.21682640762999342No Hit
CTACGCCTGTCTGAGCGTCGCT158850.2072375141517717No Hit
CTTCTCACTACTGCACTTGACTAG154790.20194079203999207No Hit
CCCATAAAGTAGAAAGCACTAC150840.19678757717754639No Hit
CATTGCACTTGTCTCGGTCTGA149110.19453059952893093No Hit
CACCCGGCTGTGTGCACATGTGC141440.1845242304162832No Hit
CAACGGAATCCCAAAAGCAGCT137850.17984067564256676No Hit
TGAGGTAGTAGTTTGTACAGTT135790.1771531762459495No Hit
TAGCAGCACGTAAATATTGGC135140.17630517886352173No Hit
ACTGGACTTGGAGTCAGAAGGCA127890.16684674652105813No Hit
TATTGCACTTGTCCCGGCCTGTAG125850.1641853393515925No Hit
CCCCCCACTGCTAAATTTGACTGGCT125070.16316774249267918No Hit
TCGTACGACTCTTAGCGG123270.16081944204903303No Hit
CAAAGAATTCTCCTTTTGGGCTT122970.1604280586417587No Hit
GACCCGCGGGCGCTCTCCAGTCCT115830.151113133548629No Hit
CGCCCCACTGCTAAATTTGACTG112470.1467296393871562No Hit
CACTAGATTGTGAGCTCCTGGAA111320.14522933632593785No Hit
TTATAAAGCAATGAGACTGATT108390.14140682504822497No Hit
TCGTTTCCCGGCCAATGCACCA107770.14059796600652463No Hit
CAACACCAGTCGATGGGCTGT105900.1381583427678478No Hit
CCCCCCACTGCTAAATTTGACTGTA103010.13438801594443817No Hit
TGAGGTAGTAGTTTGTGCTGTT102570.13381398694710245No Hit
CGCGACCTCAGATCAGACGTGGCG102150.13326605017691837No Hit
CCCCCCACTGCTAAATTTGACTGT101340.1322093149772776No Hit
TTCAAGTAATCCAGGATAGGCTT99610.12995233732866213No Hit
AGCAGCATTGTACAGGGCTATGT98460.12845203426744375No Hit
ACTGGACTTGGAGTCAGAAGGCT97600.12733006849992395No Hit
GGGCTACGCCTGTCTGAGCGTCGCT95490.12457733853542764No Hit
GCGTTCGCGCTTTCCCCTG93730.12228122254608474No Hit
TTCACAGTGGCTAAGTTCCG93610.122124669183175No Hit
CCCCCCACTGCTAAATTTGACTGA93280.12169414743517322No Hit
TATTGCACTTGTCCCGGCCTGTT92650.12087224227989707No Hit
GCCCCCACTGCTAAATTTGACTG92010.12003729101104511No Hit
TATTGCACTTGTCCCGGCCTGTAAA91800.11976332262595304No Hit
CACTAGATTGTGAGCTCCTGGA91400.11924147808292057No Hit
ATGCACCTGGGCAAGGATTCTGA90220.11770203668097477No Hit
TATTGCACTTGTCCCGGCCTGTAAT89810.11716714602436648No Hit
AGCTCGGTCTGAGGCCCCTCAGT89450.11669748593563727No Hit
TAGCAGCACGTAAATATTGGCGT89440.11668443982206145No Hit
TTAATGCTAATCGTGATAGGGGTTT88790.11583644243963367No Hit
ACGCCTGTCTGAGCGTCGCT88190.11505367562508496No Hit
TGTCAGTTTGTCAAATACCCCA87090.11361860313174567No Hit
AACGGAATCCCAAAAGCAGCTG86380.11269232906786301No Hit
ATCACATTGCCAGGGATTTCCA85410.11142685605100926No Hit
TGTCAGTTTGTCAAATACCCCAA85170.11111374932518978No Hit
GGAATACCGGGTGCTGTAGGCTTT85050.11095719596228003No Hit
TGTAAACATCCCCGACTGGAAGC84770.1105919047821573No Hit
CAAAGTGCTGTTCGTGCAGGTAG77990.10174663977775686No Hit
CTCCCACTGCTTCACTTGACTAG77080.10055944344235797No Hit
CTCGTACGACTCTTAGCGG77020.1004811667609031No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)