FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003947682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5767469
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG71046712.318523081788562No Hit
CCCCCCACTGCTAAATTTGACTG59800310.368551612501081No Hit
CCCCCACTGCTAAATTTGACTG3734216.474607839244563No Hit
TCGTACGACTCTTAGCGG3544906.146370270910862No Hit
GCCTGTCTGAGCGTCGCT2808344.869276280462019No Hit
TTCAAGTAATCCAGGATAGGCT1539932.6700273551535347No Hit
TAGCAGCACGTAAATATTGGCG1053331.826329712392039No Hit
TGAGGTAGTAGATTGTATAGTT1026911.7805210569835745No Hit
AACATTCAACGCTGTCGGTGAG979511.6983359598465113No Hit
AAGCTGCCAGTTGAAGAACTGT805401.3964531062065526No Hit
TGAGGTAGTAGGTTGTATAGTT802491.391407565432948No Hit
TAGCTTATCAGACTGATGTTGAC560930.9725756653395102No Hit
TAGCTTATCAGACTGATGTTGA531940.922310982512433No Hit
TATTGCACTTGTCCCGGCCTGT451450.7827523650322178No Hit
CATAAAGTAGAAAGCACTACT365280.6333454067980253No Hit
TAGCTTATCAGACTGATGTTGACT349750.6064185173773798No Hit
TGAGGTAGTAGTTTGTACAGTT314600.5454732396481021No Hit
TTCAAGTAATTCAGGATAGGTT287300.4981387849678949No Hit
TATTGCACTTGTCCCGGCCTGTAA283640.49179284708769133No Hit
AACCGGGCGGAAACACCA280770.48681666082643876No Hit
TCCTGTCTGAGCGTCGCT280480.48631384061188715No Hit
CACCCGGCTGTGTGCACATGTGC279790.485117475273816No Hit
AACATTCAACGCTGTCGGTGAGT277750.4815803951438664No Hit
CATTGCACTTGTCTCGGTCTGA274620.4761534045523262No Hit
TTCTCACTACTGCACTTGACTAG269690.4676054609049481No Hit
TATTGCACTTGTCCCGGCCTGTAT243920.42292381632220305No Hit
CAACGGAATCCCAAAAGCAGCT216510.3753986367330279No Hit
CAACGGAATCCCAAAAGCAGCTGT216240.3749304937746523No Hit
AACGGAATCCCAAAAGCAGCTG207040.3589789559337033No Hit
TGTAAACATCCTTGACTGGAAGCT198940.34493466718243304No Hit
TATTGCACTTGTCCCGGCCTGTA191380.33182666434791414No Hit
AGCAGCATTGTACAGGGCTATGA190480.3302661878199952No Hit
CCCATAAAGTAGAAAGCACT174310.30222962620171867No Hit
CAACACCAGTCGATGGGCTGT155410.2694596191154214No Hit
CTCCCACTGCTTCACTTGACTAG152900.26510762346533634No Hit
CCCATAAAGTAGAAAGCACTAC146180.25345606539020843No Hit
TTCACAGTGGCTAAGTTCCGC139180.24131902572861685No Hit
TGAGGTAGTAGATTGTATAGT138130.23949846977937808No Hit
ACTGGACTTGGAGTCAGAAGGC134400.2330311615025586No Hit
CCCCCACTGCTAAATTTGACTGGCTT133360.23122794418140782No Hit
ACCGGGTGCTGTAGGCTT127470.2210154922375829No Hit
CTTCTCACTACTGCACTTGACTAG124370.2156405175303066No Hit
TGAGAACTGAATTCCATGGGTT113750.1972268945008634No Hit
TAGCAGCACGTAAATATTGGC113320.1964813334930799No Hit
TGAGGTAGTAGTTTGTGCTGTT106520.18469106639324806No Hit
TGTAAACATCCCCGACTGGAAGCT105810.18346002379900092No Hit
CTCGTACGACTCTTAGCGG105670.18321728300576906No Hit
TCGTTTCCCGGCCAATGCACCA103690.17978423464434745No Hit
TGGCTCAGTTCAGCAGGAAC97030.16823670833774745No Hit
TTCACAGTGGCTAAGTTCTGC91470.15859643112082614No Hit
TGTCAGTTTGTCAAATACCCCA86590.150135180613888No Hit
TTCACAGTGGCTAAGTTCTG86400.14980574668021623No Hit
CCCATAAAGTAGAAAGCACTA86250.14954566725889643No Hit
AGCTCGGTCTGAGGCCCCTCAGT85260.1478291430781856No Hit
TGATGTAGTAGATTGTATAGTT84820.14706624344231414No Hit
ACCGGGTGCTGTAGGCTTT84170.1459392326165949No Hit
ACCCCCCACTGCTAAATTTGACTG83890.14545375103013125No Hit
CACTAGATTGTGAGCTCCTGGA81540.14137917342945408No Hit
TGAGGTAGTAAGTTGTATTGTT81340.1410324008676943No Hit
AGAATTACTCTGAGACCT78260.13569210341659402No Hit
TGAGAACTGAATTCCATAGGCTGT77320.13406227237632312No Hit
TGTAAACATCCCCGACTGGA77080.13364614530221142No Hit
GGAATACCGGGTGCTGTAGGCTTT73770.1279070594050874No Hit
GGAATACCGGGTGCTGTAGGCTT70840.12282684137530692No Hit
TTCAAGTAATCCAGGATAGGC68230.11830145944434205No Hit
GACCCGCGGGCGCTCTCCAGTCCT66950.11608211504907959No Hit
TGATGTAGTAGGTTGTATAGTT66660.11557929483452793No Hit
AATGCACCCGGGCAAGGATTCT66490.11528453815703213No Hit
TAGCTTATCAGACTGATGTTGACA66470.11524986090085618No Hit
TCCCACTGCTTCACTTGACTAG64710.11219826235737028No Hit
AGGTCCCTGTTCGGGCGCCA64050.11105391290356308No Hit
TGAGGTAGTAGGTTGTATAGT61490.10661522411303814No Hit
CACTAGATTGTGAGCTCCTGGAA61410.10647651508833424No Hit
CAAAGAATTCTCCTTTTGGGCT60540.10496805444467928No Hit
TTATAAAGCAATGAGACTGATT60540.10496805444467928No Hit
CCCCCCACTGCTAAATTTGACTGG60490.10488136130423935No Hit
ACCGGGTGCTGTAGGCTTA58370.10120557214958589No Hit
CTCCCACTGCTTCACTTGACTAGCCT58360.1011882335214979No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)