FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13965248
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG7168365.132998712231964No Hit
CAACGGAATCCCAAAAGCAGCTG6939824.969349631313386No Hit
AAGCTGCCAGTTGAAGAACTGT5317253.8074869848355No Hit
TAGCTTATCAGACTGATGTTGAC4597903.2923869307584086No Hit
TAGCAGCACGTAAATATTGGCG4251933.044650549707388No Hit
AACATTCAACGCTGTCGGTGAGT3649132.6130076601575567No Hit
TTCAAGTAATCCAGGATAGGCT3559992.5491777876053474No Hit
TAGCTTATCAGACTGATGTTGACT2944032.1081115065052907No Hit
CCCCCACTGCTAAATTTGACTG2317111.6591971728679649No Hit
TGAGGTAGTAGGTTGTATAGTT2302931.6490433968662783No Hit
TGTAAACATCCTTGACTGGAAGCT2145551.536349372384937No Hit
AACATTCAACGCTGTCGGTGAG2123381.520474251513471No Hit
TAGCTTATCAGACTGATGTTGA2007091.4372032634150143No Hit
CGCCTGTCTGAGCGTCGCT1917861.373308945175911No Hit
TATTGCACTTGTCCCGGCCTGTAA1777231.2726089790886634No Hit
TGAGGTAGTAGATTGTATAGTT1621351.160989049388883No Hit
TATTGCACTTGTCCCGGCCTGTAT1522941.0905212710866288No Hit
AGCAGCATTGTACAGGGCTATGA1518761.087528126962013No Hit
TATTGCACTTGTCCCGGCCTGT1207860.8649040819038802No Hit
CATAAAGTAGAAAGCACTACT1069570.7658797036758674No Hit
TTCAAGTAATTCAGGATAGGTT1028530.7364924704523687No Hit
ACTGGACTTGGAGTCAGAAGGC880380.6304077091935639No Hit
TGTAAACATCCCCGACTGGAAGCT871030.6237125183885026No Hit
TATTGCACTTGTCCCGGCCTGTA776200.5558082462982398No Hit
TACGCCTGTCTGAGCGTCGCT759130.5435850476840798No Hit
TGAGGTAGTAGTTTGTACAGTT700550.5016380661481987No Hit
TGTCAGTTTGTCAAATACCCCAA621030.44469672146173134No Hit
CTACGCCTGTCTGAGCGTCGCT554050.3967348091491107No Hit
TAGCTTATCAGACTGATGTTGACA486410.3483002951326034No Hit
CAACGGAATCCCAAAAGCAGCTGT479220.3431518008129895No Hit
CCCATAAAGTAGAAAGCACTAC465890.33360667852085407No Hit
CATTGCACTTGTCTCGGTCTGA460980.3300908082692123No Hit
GCGTTCGCGCTTTCCCCTG437170.31304134376990655No Hit
TGTAAACATCCTACACTCTCAGCT436940.312876649236734No Hit
AACATTCAACGCTGTCGGTGAGTT429850.3077997612358907No Hit
ACGCCTGTCTGAGCGTCGCT422610.3026154637568914No Hit
CAACACCAGTCGATGGGCTGT409260.2930560202010018No Hit
CGCGACCTCAGATCAGACGTGGCG404880.2899196634388448No Hit
TGAGGTAGTAGTTTGTGCTGTT401340.2873847997543617No Hit
TTATAAAGCAATGAGACTGATT384500.27532629567337435No Hit
CACCCGGCTGTGTGCACATGTGC367480.26313890021859976No Hit
ACCGGGTGCTGTAGGCTT361520.25887116361986556No Hit
TGTCAGTTTGTCAAATACCCCA359960.2577541050470425No Hit
TTCTCACTACTGCACTTGACTAG335000.23988116788187364No Hit
GCCTGTCTGAGCGTCGCT301090.21559946518672637No Hit
CGCGACCTCAGATCAGACGTGG286000.20479407168422648No Hit
TTCACAGTGGCTAAGTTCCG275150.19702478609760457No Hit
ACCGGGTGCTGTAGGCTTT270300.1935518796372252No Hit
TAGCAGCACGTAAATATTGGC265510.19012193696810825No Hit
AGCAGCATTGTACAGGGCTATGT265370.19002168812182926No Hit
CAACGGAATCCCAAAAGCAGCT260310.18639840839203142No Hit
CCAAGTCCTTCTGATCGAGGCCC255940.18326921226175147No Hit
CCCATAAAGTAGAAAGCACT253050.181199789649278No Hit
ACTGGACTTGGAGTCAGAAGGCA251490.18008273107645495No Hit
ACCGGGTGCTGTAGGCTTA249290.17850739206349936No Hit
CTTCTCACTACTGCACTTGACTAG237590.17012945276732644No Hit
TTCACAGTGGCTAAGTTCCGC237250.169885991283506No Hit
CAAAGAATTCTCCTTTTGGGCT232730.16664938567507No Hit
TTCAAGTAATCCAGGATAGGCTT231630.16586171616859222No Hit
TAGCAGCACGTAAATATTGGCGT231180.16553948773412402No Hit
CCTCGACACAAGGGTTTGT227980.16324808553346135No Hit
TGTAAACATCCCCGACTGGAAGC225940.16178731663053889No Hit
CAAAGTGCTGTTCGTGCAGGTAG224340.16064161553020756No Hit
TGTAAACATCCTACACTCAGCT220780.15809243058197034No Hit
CAAAGAATTCTCCTTTTGGGCTT219300.15703265706416386No Hit
TGAGGTAGTAGGTTGTATAGT217630.155836831540693No Hit
TGAGGTAGTAGGTTGTGTGGTT211730.1516120587332212No Hit
ACTGGACTTGGAGTCAGAAGG209720.15017277172592997No Hit
ACCACAGGGTAGAACCACGGAC205640.14725123392008507No Hit
TATTGCACTTGTCCCGGCCTGTT202030.14466624581246249No Hit
CAAGTCCTTCTGATCGAGGCCC197340.14130790946211624No Hit
AGCAGCATTGTACAGGGCTATG197140.14116469682457483No Hit
AACGGAATCCCAAAAGCAGCTG195030.1396538034985129No Hit
TGAGGTAGTAAGTTGTATTGTT184880.13238576214328598No Hit
CCCCCACTGCTAAATTTGACTGGCTT180790.12945706370556398No Hit
TATTGCACTTGTCCCGGCCTGTAG177580.12715850087302424No Hit
CTGCGTTCGCGCTTTCCCCTG167100.11965415866585398No Hit
AGCTCGGTCTGAGGCCCCTCAGT166950.11954674918769792No Hit
GGGCTACGCCTGTCTGAGCGTCGCT166670.11934625149513994No Hit
TCGTACGACTCTTAGCGG161920.11594495135353126No Hit
CACTAGATTGTGAGCTCCTGGA157360.11267970321758697No Hit
ATAGGCGATAGAAATTGAAACCT156050.11174166044169069No Hit
CCCCCCACTGCTAAATTTGACTGG155830.11158412654039512No Hit
TTCACAGTGGCTAAGTTCTGC154710.11078213577016319No Hit
CTCCCACTGCTTCACTTGACTAG152270.1090349415921579No Hit
TGTAAACATCCTTGACTGGAAGC151620.1085695005201483No Hit
TTCAAGTAATCCAGGATAGGC151050.10816134450315526No Hit
ATCCCACCGCTGCCACCA149790.10725910488664434No Hit
TATTGCACTTGTCCCGGCCTGTAAT143620.10284099501849162No Hit
TGAGGTAGTAGATTGTATAGT143620.10284099501849162No Hit
ATCACATTGCCAGGGATTTCCA143170.10251876658402342No Hit
ACCCCCCACTGCTAAATTTGACTG141830.10155924191249593No Hit
TCGTTCGCGCTTTCCCCTG141320.10119404968676532No Hit
TCGTTTCCCGGCCAATGCACCA141140.10106515831297803No Hit
AGAGGTAGTAGGTTGCATAGTT140490.10059971724096844No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)