FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9753458
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5994036.145543457510147No Hit
CCCCCCACTGCTAAATTTGACTG4430754.542747813134582No Hit
AAGCTGCCAGTTGAAGAACTGT4006184.107445789995713No Hit
TTCAAGTAATCCAGGATAGGCT3817513.914006704083824No Hit
TAGCAGCACGTAAATATTGGCG3105823.1843270356011173No Hit
AACATTCAACGCTGTCGGTGAGT2879012.951783869884917No Hit
TAGCTTATCAGACTGATGTTGAC2228452.2847794084928648No Hit
TAGCTTATCAGACTGATGTTGACT1904891.9530406549143904No Hit
TGAGGTAGTAGGTTGTATAGTT1754941.7993003096952898No Hit
CCCCCACTGCTAAATTTGACTG1623301.6643327935589611No Hit
TAGCTTATCAGACTGATGTTGA1582941.6229525979401356No Hit
AACATTCAACGCTGTCGGTGAG1490861.5285450555074929No Hit
CATAAAGTAGAAAGCACTACT1225531.2565082045772895No Hit
TGTAAACATCCTTGACTGGAAGCT1166751.1962423993623594No Hit
TGAGGTAGTAGATTGTATAGTT1037381.0636022629102415No Hit
GCCTGTCTGAGCGTCGCT1017711.0434350565717307No Hit
TATTGCACTTGTCCCGGCCTGTAA990311.0153424559781772No Hit
TATTGCACTTGTCCCGGCCTGT875180.8973022696155559No Hit
TATTGCACTTGTCCCGGCCTGTAT872150.8941956791119622No Hit
AGCAGCATTGTACAGGGCTATGA743410.7622014674180172No Hit
TTCAAGTAATTCAGGATAGGTT710210.7281622579396968No Hit
CCCATAAAGTAGAAAGCACTAC605450.620754198152081No Hit
ACTGGACTTGGAGTCAGAAGGC588120.6029861409153553No Hit
TGTAAACATCCCCGACTGGAAGCT584720.5995001977760093No Hit
AACCGGGCGGAAACACCA508460.5213125437152649No Hit
CGCCTGTCTGAGCGTCGCT507700.5205333328958817No Hit
TATTGCACTTGTCCCGGCCTGTA504870.517631797871073No Hit
TGAGGTAGTAGTTTGTACAGTT458540.47013069621051323No Hit
CAACGGAATCCCAAAAGCAGCTGT448840.46018550548943765No Hit
TTAATGCTAATCGTGATAGGGGTT437040.448087232241119No Hit
TCCTGTCTGAGCGTCGCT378580.38814951579224516No Hit
TGTCAGTTTGTCAAATACCCCAA365020.37424675433061794No Hit
GGAATACCGGGTGCTGTAGGCTTT363900.3730984436494216No Hit
TGTAAACATCCTACACTCTCAGCT356680.36569594086528084No Hit
CATTGCACTTGTCTCGGTCTGA346350.35510482538603233No Hit
CCCATAAAGTAGAAAGCACT333290.3417147026213677No Hit
TGAGGTAGTAGTTTGTGCTGTT287800.2950748339717052No Hit
AACATTCAACGCTGTCGGTGAGTT280350.28743651738696163No Hit
CAACACCAGTCGATGGGCTGT275140.28209482216461074No Hit
GGAATACCGGGTGCTGTAGGCTT268900.2756970912265168No Hit
TAGCTTATCAGACTGATGTTGACA267120.27387209746532976No Hit
ACCGGGTGCTGTAGGCTT262760.26940188802781534No Hit
CGCGACCTCAGATCAGACGTGGCG242590.2487220429923418No Hit
TCGTACGACTCTTAGCGG240600.24668174097843043No Hit
TTCTCACTACTGCACTTGACTAG239550.24560519971480885No Hit
TTCACAGTGGCTAAGTTCCG238690.24472346115603305No Hit
TTATAAAGCAATGAGACTGATT236570.24254987308091142No Hit
TGTCAGTTTGTCAAATACCCCA231130.2369723640579577No Hit
CTACGCCTGTCTGAGCGTCGCT228940.23472700656526124No Hit
TACGCCTGTCTGAGCGTCGCT228910.2346962482434435No Hit
CACCCGGCTGTGTGCACATGTGC217020.22250570002967152No Hit
CAAAGAATTCTCCTTTTGGGCT213690.21909152630790024No Hit
CGCGACCTCAGATCAGACGTGG207420.2126630370479885No Hit
TTCAAGTAATCCAGGATAGGCTT197330.2023179881432821No Hit
ACCGGGTGCTGTAGGCTTT194010.19891406719545007No Hit
CCCCCACTGCTAAATTTGACTGGCTT185290.18997364832042132No Hit
ACCGGGTGCTGTAGGCTTA178020.1825198816665843No Hit
TTCACAGTGGCTAAGTTCCGC169300.17357946279155556No Hit
TTCAAGTAATCCAGGATAGGC169080.173353901764892No Hit
TAGCAGCACGTAAATATTGGC167080.17130334697704136No Hit
CAACGGAATCCCAAAAGCAGCT164810.1689759672928309No Hit
ACTGGACTTGGAGTCAGAAGGCA164550.16870939517041034No Hit
TGTAAACATCCCCGACTGGAAGC164490.16864787852677482No Hit
TGAATACCGGGTGCTGTAGGCTTT159290.1633164360783632No Hit
CAAAGAATTCTCCTTTTGGGCTT158900.1629165778947323No Hit
AACGGAATCCCAAAAGCAGCTG157300.16127613406445182No Hit
TGAGGTAGTAGGTTGTATAGT151530.15536028350150274No Hit
TATTGCACTTGTCCCGGCCTGTT147960.15170004320518937No Hit
AGCAGCATTGTACAGGGCTATGT147100.1508183046464136No Hit
CTTCTCACTACTGCACTTGACTAG145240.1489112886937125No Hit
ACTGGACTTGGAGTCAGAAGG145020.14868572766704896No Hit
TGTAAACATCCTACACTCAGCT144350.14799879181311898No Hit
TGAGGTAGTAAGTTGTATTGTT140840.14440006816044115No Hit
TGAGGTAGTAGGTTGTGTGGTT139890.1434260546362121No Hit
TAGCAGCACGTAAATATTGGCGT139200.14271861323440363No Hit
CACTAGATTGTGAGCTCCTGGA137860.14134474152654372No Hit
ACGCCTGTCTGAGCGTCGCT137070.14053477238534273No Hit
ACTGGACTTGGAGTCAGAAGGCT132920.13627987120055268No Hit
ACCACAGGGTAGAACCACGGAC124450.12759577167400526No Hit
TGAGAACTGAATTCCATGGGTT122660.12576052513887895No Hit
TTCACAGTGGCTAAGTTCTGC119880.12291025398376658No Hit
TGAATACCGGGTGCTGTAGGCTT119800.12282823179225255No Hit
CAAAGTGCTGTTCGTGCAGGTAG118930.12193624045953753No Hit
ATCCCGGACGAGCCCCCA118560.12155688782378515No Hit
AGAGGTAGTAGGTTGCATAGTT116610.1195575969056308No Hit
AGCAGCATTGTACAGGGCTATG114480.11737375605656988No Hit
CCCCCCACTGCTAAATTTGACTGG112170.1150053652766024No Hit
TATTGCACTTGTCCCGGCCTGTAG109200.11196029141664424No Hit
CACTAGATTGTGAGCTCCTGGAA109150.11190902754694795No Hit
CAAAGTGCTGTTCGTGCAGGT108490.11123234446695726No Hit
CCCCCCACTGCTAAATTTGACTGGCT107400.11011479210757866No Hit
CCCATAAAGTAGAAAGCACTA105250.10791044571063925No Hit
TTCACAGTGGCTAAGTTCTG104230.10686466276883541No Hit
CGCGACCTCAGATCAGACGTGGC103760.10638278239369053No Hit
ACCCCCCACTGCTAAATTTGACTG102820.10541902164340072No Hit
AGCTCGGTCTGAGGCCCCTCAGT102730.10532674667794745No Hit
CTCCCACTGCTTCACTTGACTAG100890.10344023627312487No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)