FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9921953
Sequences flagged as poor quality0
Sequence length18-26
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG100561010.135202212709533No Hit
CCCCCCACTGCTAAATTTGACTG6351646.40160258771635No Hit
TTCAAGTAATCCAGGATAGGCT3085813.110083266872963No Hit
CCCCCACTGCTAAATTTGACTG2479842.499346650805542No Hit
AACATTCAACGCTGTCGGTGAGT2392952.411773166028906No Hit
AAGCTGCCAGTTGAAGAACTGT2200132.2174364260745842No Hit
TAGCAGCACGTAAATATTGGCG1789271.8033445633132912No Hit
AACATTCAACGCTGTCGGTGAG1367081.3778335777240631No Hit
CATAAAGTAGAAAGCACTACT1042161.0503577269515387No Hit
ACCGGGTGCTGTAGGCTTA922980.9302402460483336No Hit
CGCCTGTCTGAGCGTCGCT895230.9022719619816784No Hit
ATCCCGGACGAGCCCCCA894210.9012439385673365No Hit
TCGTACGACTCTTAGCGG868820.8756542184789627No Hit
ACCGGGTGCTGTAGGCTT700530.7060404337734718No Hit
TAGCTTATCAGACTGATGTTGA675350.6806623655645214No Hit
CAACGGAATCCCAAAAGCAGCTGT669020.6742825731990466No Hit
CCCATAAAGTAGAAAGCACTAC642300.6473523912076584No Hit
CCCATAAAGTAGAAAGCACT620180.6250583932417337No Hit
CAACACCAGTCGATGGGCTGT589190.5938246230354044No Hit
TAGCTTATCAGACTGATGTTGAC588640.5932702966845338No Hit
ACCGGGTGCTGTAGGCTTT586040.5906498448440544No Hit
TATTGCACTTGTCCCGGCCTGT565720.5701700058446155No Hit
GCCTGTCTGAGCGTCGCT561250.5656648444111759No Hit
AGCAGCATTGTACAGGGCTATGA550000.5543263508706401No Hit
ACTGGACTTGGAGTCAGAAGGC530150.5343202089346725No Hit
CACCCGGCTGTGTGCACATGTGC511050.5150699665680739No Hit
CATTGCACTTGTCTCGGTCTGA500110.5040439115162105No Hit
TTCAAGTAATTCAGGATAGGTT483670.4874745929556409No Hit
TATTGCACTTGTCCCGGCCTGTAA462770.46641019162255654No Hit
TGAGGTAGTAGGTTGTATAGTT430320.43370493692118883No Hit
TAGCTTATCAGACTGATGTTGACT424670.4280104934986086No Hit
TATTGCACTTGTCCCGGCCTGTAT405710.40890135238495884No Hit
CAACGGAATCCCAAAAGCAGCT395310.39841954502304133No Hit
TGAGGTAGTAGATTGTATAGTT316510.318999696934666No Hit
AACCGGGCGGAAACACCA315240.31771970699720103No Hit
CTACGCCTGTCTGAGCGTCGCT295440.297763958365858No Hit
CACTAGATTGTGAGCTCCTGGA254450.25645152723460796No Hit
TACGCCTGTCTGAGCGTCGCT250700.2526720293877627No Hit
TGTAAACATCCTACACTCTCAGCT249410.2513718821284479No Hit
TATTGCACTTGTCCCGGCCTGTA246940.24888245287999244No Hit
AGCTCGGTCTGAGGCCCCTCAGT240950.24284533498596497No Hit
TTCACAGTGGCTAAGTTCCG236220.23807812836847747No Hit
TCCTGTCTGAGCGTCGCT227950.229743075783568No Hit
ACGCCTGTCTGAGCGTCGCT227000.22878560299570055No Hit
CTCCCACTGCTTCACTTGACTAG212360.2140304434016166No Hit
TTCTCACTACTGCACTTGACTAG203900.20550389625913365No Hit
AACGGAATCCCAAAAGCAGCTG196830.1983782829852147No Hit
TGTAAACATCCTTGACTGGAAGCT196660.19820694574949105No Hit
AACATTCAACGCTGTCGGTGAGTT191330.1928350194765083No Hit
CACTAGATTGTGAGCTCCTGGAA187140.1886120605489665No Hit
CAAAGAATTCTCCTTTTGGGCT185530.18698939614005428No Hit
CGCGACCTCAGATCAGACGT179460.1808716489586274No Hit
TTCACAGTGGCTAAGTTCCGC175710.17709215111178211No Hit
ATCCCACCGCTGCCACCA171390.17273816959221638No Hit
TGTAAACATCCCCGACTGGAAGCT169250.17058133615428334No Hit
ACTGGACTTGGAGTCAGAAGGCT161050.16231683419584833No Hit
TTCAAGTAATCCAGGATAGGCTT159410.16066393380416133No Hit
TCGTTTCCCGGCCAATGCACCA158490.15973669699906864No Hit
CCCATAAAGTAGAAAGCACTA155390.1566123121123432No Hit
TGAGAACTGAATTCCATGGGTT152890.15409264688111302No Hit
ACCTGGTGCTGTAGGCTTA151340.15253045443775032No Hit
CCCCCACTGCTAAATTTGACTGGCTT149270.15044417162629173No Hit
ACCACAGGGTAGAACCACGGAC147550.14871064194720537No Hit
CGCGACCTCAGATCAGACGTGGC146190.14733994406141612No Hit
CTTCTCACTACTGCACTTGACTAG143640.14476988552556136No Hit
ACTGGACTTGGAGTCAGAAGG140460.14156487135143656No Hit
CAAAGAATTCTCCTTTTGGGCTT137840.13892426218910733No Hit
CCCCCCACTGCTAAATTTGACTGG131140.13217155936941044No Hit
TCGTTTCCCGGGCGGCGCACCA129830.13085125478824583No Hit
CAAAGTGCTGTTCGTGCAGGT129220.13023645647182566No Hit
CGCGACCTCAGATCAGACGTGG127930.1289363092125109No Hit
TTAATGCTAATCGTGATAGGGGTT125820.12680971175735262No Hit
TTCAAGTAATCCAGGATAGGC123980.12495523814716719No Hit
CGCGACCTCAGATCAGACGTGGCG117360.11828316461486967No Hit
AGCAGCATTGTACAGGGCTATGT116660.11757765835012522No Hit
TGTCAGTTTGTCAAATACCCCAA114680.11558208348699092No Hit
TGTAAACATCCTACACTCAGCT114130.11502775713612028No Hit
ACTGGACTTGGAGTCAGAAGGCA113510.1144028801587752No Hit
AGCTACATTGTCTGCTGGGTTT112730.11361674460663136No Hit
ACCTGGTGCTGTAGGCTT112320.11320351950870962No Hit
ACTTGACTTGGAGTCAGAAGGC110120.11098621410522706No Hit
TAGCAGCACGTAAATATTGGC108850.10970622416776213No Hit
TACCACCCTGAACGCGCCCGA108640.10949457228833881No Hit
ATACCGGGTGCTGTAGGCTTA108210.10906118986856721No Hit
CAACGGAATCCCAAAAGCAGCTGA108030.10887977397191864No Hit
CCCCCCACTGCTAAATTTGACTGGCT105400.10622908614866448No Hit
GGAATACCGGGTGCTGTAGGCTTT105080.10590656899906702No Hit
ACCCCCCACTGCTAAATTTGACTG104010.1048281522801005No Hit
TGTCAGTTTGTCAAATACCCCA102980.10379005020483367No Hit
CACCCGTAGAACCGACCTTGCG102670.10347761171616111No Hit
AGCTACATCTGGCTACTGGGTCTC102560.10336674644598699No Hit
CCACCCTGAACGCGCCCGA101080.10187510462909874No Hit
TGAGAACTGAATTCCATAGGCTGT100820.1016130594450508No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)