FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8929350
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG5832276.531572846847755No Hit
CAACGGAATCCCAAAAGCAGCTG5416116.06551428715416No Hit
AAGCTGCCAGTTGAAGAACTGT3308953.7057008628847568No Hit
TAGCAGCACGTAAATATTGGCG3154553.532787940891554No Hit
TTCAAGTAATCCAGGATAGGCT2627822.9429017789648744No Hit
AACATTCAACGCTGTCGGTGAGT2209872.4748385940745967No Hit
TAGCTTATCAGACTGATGTTGAC1862342.0856389322851046No Hit
CCCCCACTGCTAAATTTGACTG1778631.9918919070257075No Hit
TAGCTTATCAGACTGATGTTGACT1408651.577550437601841No Hit
AACATTCAACGCTGTCGGTGAG1282191.4359275871144037No Hit
TAGCTTATCAGACTGATGTTGA1141071.277886968256368No Hit
TGTAAACATCCTTGACTGGAAGCT995311.1146500025197803No Hit
TGAGGTAGTAGGTTGTATAGTT951501.0655870808065535No Hit
TATTGCACTTGTCCCGGCCTGTAA934841.0469295077469245No Hit
TATTGCACTTGTCCCGGCCTGTAT815690.9134931433978958No Hit
TTAATGCTAATCGTGATAGGGGTT777000.8701641216885887No Hit
CATAAAGTAGAAAGCACTACT682230.7640309764988493No Hit
TATTGCACTTGTCCCGGCCTGT675340.7563148493451372No Hit
AGCAGCATTGTACAGGGCTATGA632890.7087749948204517No Hit
TGAGGTAGTAGATTGTATAGTT626640.7017756051672294No Hit
TTCAAGTAATTCAGGATAGGTT557780.6246591297238882No Hit
TGTAAACATCCCCGACTGGAAGCT515750.5775896341838992No Hit
TATTGCACTTGTCCCGGCCTGTA497860.5575545812405158No Hit
CGCCTGTCTGAGCGTCGCT432810.4847049337297788No Hit
CAACGGAATCCCAAAAGCAGCTGT397310.4449484004994764No Hit
ACTGGACTTGGAGTCAGAAGGC392930.44004322823049835No Hit
TGTAAACATCCTACACTCTCAGCT372660.417342807707168No Hit
GCCTGTCTGAGCGTCGCT360410.40362400398685233No Hit
CCCATAAAGTAGAAAGCACTAC354090.396546221169514No Hit
TGTCAGTTTGTCAAATACCCCAA318590.3567896879392117No Hit
TTCTCACTACTGCACTTGACTAG305420.34204057406194177No Hit
TTCACAGTGGCTAAGTTCCG293190.32834416838851654No Hit
ACCGGGTGCTGTAGGCTT291600.3265635236607368No Hit
CAAAGAATTCTCCTTTTGGGCT280860.3145357724806397No Hit
AACATTCAACGCTGTCGGTGAGTT272910.305632548841741No Hit
TGAGGTAGTAGTTTGTACAGTT258340.28931557168214933No Hit
CATTGCACTTGTCTCGGTCTGA247050.27667187421256867No Hit
TAGCTTATCAGACTGATGTTGACA245130.27452166171109876No Hit
CTACGCCTGTCTGAGCGTCGCT233100.26104923650657663No Hit
CCCCCACTGCTAAATTTGACTGGCTT232840.26075806189700257No Hit
TGTCAGTTTGTCAAATACCCCA232220.2600637224434029No Hit
ACCGGGTGCTGTAGGCTTT218300.24447468180774637No Hit
TCGTACGACTCTTAGCGG214060.2397262958670004No Hit
CCCATAAAGTAGAAAGCACT210610.23586263277842173No Hit
GGGCTACGCCTGTCTGAGCGTCGCT205850.23053189761852766No Hit
ACCGGGTGCTGTAGGCTTA202250.22650024917827163No Hit
TACGCCTGTCTGAGCGTCGCT201170.22529075464619483No Hit
CTTCTCACTACTGCACTTGACTAG193380.21656671538241865No Hit
TGAGAACTGAATTCCATAGGCTGT192230.21527882768622575No Hit
AACCGGGCGGAAACACCA187840.21036245639380244No Hit
TGAGGTAGTAGTTTGTGCTGTT183350.20533409486692764No Hit
TAGCAGCACGTAAATATTGGC182680.20458376029610217No Hit
CAACACCAGTCGATGGGCTGT174920.19589331810266147No Hit
TGTAAACATCCTACACTCAGCT164730.18448151321204792No Hit
CACCCGGCTGTGTGCACATGTGC162790.18230890266368774No Hit
GGAATACCGGGTGCTGTAGGCTTT159520.17864682199712187No Hit
CCCCCCACTGCTAAATTTGACTGG151310.1694524237486491No Hit
CAACGGAATCCCAAAAGCAGCT150360.16838851652135933No Hit
TAGCAGCACGTAAATATTGGCGT149470.16739180343474047No Hit
TGAGAACTGAATTCCATGGGTT148470.16627190109022494No Hit
TTAATGCTAATCGTGATAGGGGTTT146220.16375212081506493No Hit
CAAAGAATTCTCCTTTTGGGCTT146190.16371852374472948No Hit
ACCCCCCACTGCTAAATTTGACTG145410.16284499991600732No Hit
ACGCCTGTCTGAGCGTCGCT141100.15801822081114525No Hit
TGTAAACATCCCCGACTGGAAGC141030.15793982764702919No Hit
TATTGCACTTGTCCCGGCCTGTT135480.15172436963496783No Hit
GCGTTCGCGCTTTCCCCTG130570.14622564912339645No Hit
CCCCCCACTGCTAAATTTGACTGGCT130000.14558730478702256No Hit
TTATAAAGCAATGAGACTGATT129960.14554250869324195No Hit
GGAATACCGGGTGCTGTAGGCTT129300.14480337314586167No Hit
ACTGGACTTGGAGTCAGAAGGCA128410.14380666005924284No Hit
TCGTTTCCCGGCCAATGCACCA128020.14336989814488177No Hit
TTCAAGTAATCCAGGATAGGC124330.13923745849361935No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT124040.13891268681370986No Hit
AACGGAATCCCAAAAGCAGCTG123110.1378711776333104No Hit
AGCTACATTGTCTGCTGGGTTT122550.13724403232038165No Hit
ATCACATTGCCAGGGATTTCCA121420.1359785426710791No Hit
CGCGACCTCAGATCAGACGTGGCG120530.13498182958446023No Hit
CTCCCACTGCTTCACTTGACTAG112860.1263921786020259No Hit
ACTGGACTTGGAGTCAGAAGG111000.12430916024122696No Hit
TTCAAGTAATCCAGGATAGGCTT110690.12396199051442713No Hit
TATTGCACTTGTCCCGGCCTGTAG109830.12299887449814377No Hit
AGCAGCATTGTACAGGGCTATG109530.12266290379478909No Hit
AGCAGCATTGTACAGGGCTATGT107890.12082626394978359No Hit
TAGCAGCACATCATGGTTTACA107680.12059108445743531No Hit
ATCCCGGACGAGCCCCCA107340.12021031766030002No Hit
CGCGACCTCAGATCAGACGTGG105970.11867605144831371No Hit
TTAATGCTAATCGTGATAGGGGT105770.1184520709794106No Hit
CAAAGTGCTGTTCGTGCAGGTAG105590.11825048855739778No Hit
TGAGGTAGTAGGTTGTGTGGTT103870.11632425652483103No Hit
AGCTCGGTCTGAGGCCCCTCAGT101840.11405085476546445No Hit
CACTAGATTGTGAGCTCCTGGAA100300.11232620515491049No Hit
CACTAGATTGTGAGCTCCTGGA99860.11183344812332366No Hit
TGATGTAGTAGGTTGTATAGTT99800.11176625398265272No Hit
ATCACATTGCCAGGGATTTCC96180.10771220749550639No Hit
AGCTACATCTGGCTACTGGGTCT94390.10570758229882354No Hit
TTCACAGTGGCTAAGTTCTG93660.1048900535873272No Hit
CCCATAAAGTAGAAAGCACTA92150.10319900104710869No Hit
TGAGGTAGTAGGTTGTATAGT92000.10303101569543137No Hit
ACTGGACTTGGAGTCAGAAGGCT91730.10272864206241215No Hit
TTCACAGTGGCTAAGTTCCGC91410.10237027331216716No Hit
ACCACAGGGTAGAACCACGGAC90360.10119437585042584No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)