FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003947687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5574873
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG91854116.476447086776684No Hit
CCCCCCACTGCTAAATTTGACTG3842186.891959691279066No Hit
TTCAAGTAATCCAGGATAGGCT1766383.1684667973602267No Hit
CCCCCACTGCTAAATTTGACTG1416702.5412238090446184No Hit
TAGCAGCACGTAAATATTGGCG1276212.28921806828604No Hit
AACATTCAACGCTGTCGGTGAG1102591.9777849647875385No Hit
TGAGGTAGTAGATTGTATAGTT1000251.7942112762030633No Hit
TAGCTTATCAGACTGATGTTGAC989531.7749821386065654No Hit
AAGCTGCCAGTTGAAGAACTGT869701.560035538029297No Hit
TCGTACGACTCTTAGCGG810211.4533245869457474No Hit
TAGCTTATCAGACTGATGTTGACT685171.2290324819955538No Hit
TAGCTTATCAGACTGATGTTGA661141.1859283610586286No Hit
TATTGCACTTGTCCCGGCCTGTAA619651.1115051410139747No Hit
TGAGGTAGTAGGTTGTATAGTT596011.069100587582892No Hit
TATTGCACTTGTCCCGGCCTGTAT521180.9348733146028618No Hit
CCCCCACTGCTAAATTTGACTGGCTT431850.7746364805081658No Hit
TATTGCACTTGTCCCGGCCTGT429810.7709772043237577No Hit
TTCAAGTAATTCAGGATAGGTT393400.7056662994834142No Hit
CAACGGAATCCCAAAAGCAGCTGT387720.6954777265778073No Hit
TGTAAACATCCTTGACTGGAAGCT370270.6641765650984337No Hit
CACCCGGCTGTGTGCACATGTGC315360.5656810478014477No Hit
TGAGGTAGTAGTTTGTACAGTT314930.5649097297821852No Hit
AACATTCAACGCTGTCGGTGAGT312710.5609275762873881No Hit
TTCTCACTACTGCACTTGACTAG297770.5341287595251049No Hit
AGCAGCATTGTACAGGGCTATGA287740.5161373182850981No Hit
CAACGGAATCCCAAAAGCAGCT279110.5006571450147833No Hit
TATTGCACTTGTCCCGGCCTGTA274010.4915089545537629No Hit
CATAAAGTAGAAAGCACTACT261960.46989411238605794No Hit
GCCTGTCTGAGCGTCGCT261030.46822591294904836No Hit
TCGTTTCCCGGCCAATGCACCA213770.38345268134359295No Hit
TGTAAACATCCCCGACTGGAAGCT209560.3759009398061624No Hit
AACGGAATCCCAAAAGCAGCTG194440.3487792457334903No Hit
CATTGCACTTGTCTCGGTCTGA191390.343308269085233No Hit
CCCATAAAGTAGAAAGCACTAC187490.33631259402680563No Hit
ACTGGACTTGGAGTCAGAAGGC184120.33026761327119025No Hit
CAAAGAATTCTCCTTTTGGGCT181170.32497601290648237No Hit
TGAGAACTGAATTCCATGGGTT162950.2922936540437782No Hit
GGAATACCGGGTGCTGTAGGCTTT159940.2868944279089407No Hit
TGATGTAGTAGATTGTATAGTT153790.27586278647065143No Hit
CTTCTCACTACTGCACTTGACTAG153400.2751632189648087No Hit
TAGCTTATCAGACTGATGTTGACA152170.27295689067715084No Hit
CTCCCACTGCTTCACTTGACTAG147740.26501052131591163No Hit
ACCGGGTGCTGTAGGCTT140810.2525797448659369No Hit
TGAGAACTGAATTCCATAGGCTGT140260.25159317530641506No Hit
TTCACAGTGGCTAAGTTCCGC136950.24565582032092928No Hit
TAGCAGCACGTAAATATTGGC136490.24483068941660194No Hit
GGAATACCGGGTGCTGTAGGCTT132510.23769151333133506No Hit
CCCATAAAGTAGAAAGCACT132000.23677669428523304No Hit
ACCCCCCACTGCTAAATTTGACTG125190.2245611693755176No Hit
CTCCCACTGCTTCACTTGACTAGCCT116910.2097088130976257No Hit
CCCCCCACTGCTAAATTTGACTGGCT110320.197887916011719No Hit
ACCGGGTGCTGTAGGCTTT106910.19177118474268381No Hit
CACTAGATTGTGAGCTCCTGGAA101850.1826947447950832No Hit
GTCAGGATGGCCGAGCGGTCTAAGG100190.1797170984881629No Hit
CCCATAAAGTAGAAAGCACTA98010.17580669550678552No Hit
TGAGGTAGTAGATTGTATAGT97640.17514300325765267No Hit
TGTCAGTTTGTCAAATACCCCA95340.17101734873601604No Hit
TGTAAACATCCTACACTCTCAGCT94590.1696720266093954No Hit
CACTAGATTGTGAGCTCCTGGA92610.1661203761951169No Hit
TGATGTAGTAGGTTGTATAGTT91200.1635911705970701No Hit
GGGAATACCGGGTGCTGTAGGCTTT90690.16267635155096807No Hit
GGGAATACCGGGTGCTGTAGGCTT90250.16188709590335063No Hit
ACCGGGTGCTGTAGGCTTA86740.155590988350766No Hit
ATGCACCTGGGCAAGGATTCTGA84320.15125008228887007No Hit
CAAAGAATTCTCCTTTTGGGC84140.15092720497848114No Hit
CAACACCAGTCGATGGGCTGT81740.14662217417329507No Hit
TCGTTTCCCGGCCAACGCACCA81480.14615579583606658No Hit
AGCTCGGTCTGAGGCCCCTCAGT81040.14536654018844913No Hit
ATCACATTGCCAGGGATTTCC78160.14020050322222588No Hit
TTCACAGTGGCTAAGTTCTGC75750.13587753478868486No Hit
TTCAAGTAATCCAGGATAGGC75380.13521384253955201No Hit
TATTGCACTTGTCCCGGCCTGTAG73330.13153662872678892No Hit
CAAAGAATTCTCCTTTTGGGCTT72480.13001193031661887No Hit
AACCGGGCGGAAACACCA72160.12943792620926073No Hit
TTAATGCTAATCGTGATAGGGGTT72110.12934823806748602No Hit
AATGCACCCGGGCAAGGATTCT70980.1273212860633776No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC70610.12665759381424474No Hit
TGAGGTAGTAAGTTGTATTGTT69880.12534814694433397No Hit
CCCCCCACTGCTAAATTTGACTGG69750.12511495777571974No Hit
TGTAAACATCCCCGACTGGA68500.12287275423135201No Hit
TAGCAGCACATCATGGTTTACA68250.12242431352247846No Hit
TATTGCACTTGTCCCGGCCTGTAC67460.12100724088243804No Hit
TGAGGTAGTAGTTTGTGCTGTT67190.1205229249168546No Hit
ACTGGACTTGGAGTCAGAAGGCA67130.12041529914672497No Hit
AATCCTTGGAACCTAGGTGTGAGT66770.11976954452594706No Hit
TATTGCACTTGTCCCGGCCTGTT65940.11828072137248687No Hit
ACTGGACTTGGAGTCAGAAGGCT64240.11523132455214674No Hit
TGAGGTAGTAGATTGTATAGTTA63830.11449588178959412No Hit
TTCACAGTGGCTAAGTTCTG63240.11343756171665255No Hit
CAAAGTGCTGTTCGTGCAGGTAG61530.11037022726795749No Hit
TTCACAGTGGCTAAGTTCCG61370.11008322521427842No Hit
CTACGGGGATGATTTTACGAACTGAA60010.10764370775800632No Hit
TGTAAACATCCCCGACTGGAAGC58510.10495306350476505No Hit
TCGATTCCCGGCCAACGCACCA58150.10430730888398712No Hit
TGGCTCAGTTCAGCAGGAAC57670.10344630272294993No Hit
TTCAAGTAATCCAGGATAGGCTT57540.10321311355433568No Hit
TTATAAAGCAATGAGACTGATT57420.10299786201407637No Hit
TATTGCACTTGTCCCGGCCTGTAAA56050.10054040692944935No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)