FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7694421
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG6486048.42953615353254No Hit
CAACGGAATCCCAAAAGCAGCTG5353726.957924449416011No Hit
AACATTCAACGCTGTCGGTGAGT2943573.825589995660492No Hit
TTCAAGTAATCCAGGATAGGCT2502063.25178463720662No Hit
AAGCTGCCAGTTGAAGAACTGT2339853.04096955443431No Hit
TAGCAGCACGTAAATATTGGCG2149992.79421934411959No Hit
CCCCCACTGCTAAATTTGACTG2040862.6523893090851147No Hit
AACATTCAACGCTGTCGGTGAG1578042.0508885593860797No Hit
TGAGGTAGTAGGTTGTATAGTT1212091.5752842221656442No Hit
TAGCTTATCAGACTGATGTTGAC1182491.5368147908725036No Hit
TATTGCACTTGTCCCGGCCTGTAA1050731.365573835900063No Hit
TAGCTTATCAGACTGATGTTGACT1050111.3647680572716256No Hit
TATTGCACTTGTCCCGGCCTGTAT924431.2014289314296684No Hit
TGTAAACATCCTTGACTGGAAGCT783741.018582164921831No Hit
GCCTGTCTGAGCGTCGCT754980.981204433705928No Hit
TGAGGTAGTAGATTGTATAGTT717360.9323118659610645No Hit
TAGCTTATCAGACTGATGTTGA704900.9161183148153708No Hit
TATTGCACTTGTCCCGGCCTGT637670.8287433193478756No Hit
CGCCTGTCTGAGCGTCGCT635480.8258971012893628No Hit
CATAAAGTAGAAAGCACTACT618630.8039981175971526No Hit
AGCAGCATTGTACAGGGCTATGA503270.6540713069898307No Hit
ACTGGACTTGGAGTCAGAAGGC463300.6021245783145996No Hit
TATTGCACTTGTCCCGGCCTGTA429540.5582486323532335No Hit
TTCAAGTAATTCAGGATAGGTT414720.5389879238476813No Hit
TGTAAACATCCCCGACTGGAAGCT397970.5172189044503803No Hit
CCCATAAAGTAGAAAGCACTAC369790.480594966144951No Hit
CAACGGAATCCCAAAAGCAGCTGT349870.45470607859902645No Hit
GGAATACCGGGTGCTGTAGGCTTT332900.4326511377529252No Hit
TGAGGTAGTAGTTTGTACAGTT317220.41227273631115324No Hit
TTCTCACTACTGCACTTGACTAG287130.37316647997295704No Hit
CTTCTCACTACTGCACTTGACTAG257570.33474903439777987No Hit
CCCCCACTGCTAAATTTGACTGGCTT253390.32931652687057283No Hit
TGTCAGTTTGTCAAATACCCCAA250360.32537860873482227No Hit
CATTGCACTTGTCTCGGTCTGA240080.3120182792181504No Hit
TGTAAACATCCTACACTCTCAGCT237850.3091200754416739No Hit
CTCCCACTGCTTCACTTGACTAG236670.30758649676174465No Hit
CACCCGGCTGTGTGCACATGTGC232950.3027518249911202No Hit
AACATTCAACGCTGTCGGTGAGTT226760.294707035136237No Hit
CCCATAAAGTAGAAAGCACT225870.29355035291154463No Hit
GGAATACCGGGTGCTGTAGGCTT225200.2926795921356526No Hit
CCCCCCACTGCTAAATTTGACTGGCT224160.29132796346859624No Hit
TCGTACGACTCTTAGCGG221160.28742903462131847No Hit
TCCTGTCTGAGCGTCGCT216470.2813337091900742No Hit
TACGCCTGTCTGAGCGTCGCT200320.2603444755622288No Hit
AACCGGGCGGAAACACCA192010.24954444265526932No Hit
CCCCCCACTGCTAAATTTGACTGG185030.240472934870603No Hit
CTACGCCTGTCTGAGCGTCGCT184450.23971914196012928No Hit
TGAGGTAGTAGTTTGTGCTGTT161630.21006128986183625No Hit
CAACACCAGTCGATGGGCTGT159920.20783890041888792No Hit
CTCCCACTGCTTCACTTGACTAGCCT155910.20262733219302662No Hit
ACCCCCCACTGCTAAATTTGACTG150560.19567424241538123No Hit
TGTCAGTTTGTCAAATACCCCA144310.1875514739835525No Hit
TAGCTTATCAGACTGATGTTGACA143360.18631681318191454No Hit
AACGGAATCCCAAAAGCAGCTG143130.18601789530362323No Hit
TTCAAGTAATCCAGGATAGGCTT139340.1810922485265623No Hit
CAAAGAATTCTCCTTTTGGGCT136380.1772453053972482No Hit
ACGCCTGTCTGAGCGTCGCT134410.1746850087875358No Hit
TGAGGTAGTAGGTTGTGTGGTT130680.16983734058742042No Hit
CAACGGAATCCCAAAAGCAGCT125390.1629622293867206No Hit
TAGCAGCACGTAAATATTGGC122670.15942720056518872No Hit
CAAAGAATTCTCCTTTTGGGCTT120700.1568669039554763No Hit
TTATAAAGCAATGAGACTGATT115840.15055063922288628No Hit
TATTGCACTTGTCCCGGCCTGTAG115450.1500437784727402No Hit
TGTAAACATCCCCGACTGGAAGC113750.14783438545928276No Hit
ACTGGACTTGGAGTCAGAAGGCA113270.1472105568437183No Hit
TATTGCACTTGTCCCGGCCTGTT111900.14543004600346146No Hit
AGCTCGGTCTGAGGCCCCTCAGT108310.14076432781621903No Hit
TGAATACCGGGTGCTGTAGGCTTT107160.13926973842476256No Hit
CACTAGATTGTGAGCTCCTGGA107120.13921775270679884No Hit
TTCACAGTGGCTAAGTTCCGC105080.13656648109064998No Hit
CGCGACCTCAGATCAGACGTGGCG102640.13339535229486402No Hit
TGAGGTAGTAGGTTGTATAGT102590.1333303701474094No Hit
ACTGGACTTGGAGTCAGAAGGCT101770.13226466292915348No Hit
AGCAGCATTGTACAGGGCTATGT101640.13209570934577144No Hit
TTCAAGTAATCCAGGATAGGC101390.1317707986084983No Hit
CACTAGATTGTGAGCTCCTGGAA100250.13028920564653274No Hit
GCGTTCGCGCTTTCCCCTG95760.12445380880510698No Hit
AGAGGTAGTAGGTTGCATAGTT95360.12393395162546993No Hit
ACCGGGTGCTGTAGGCTT95360.12393395162546993No Hit
TTCACAGTGGCTAAGTTCTGC92620.12037292994495623No Hit
TGTAAACATCCTACACTCAGCT92340.1200090299192103No Hit
CAGGTCCCTGTTCGGGCGCCA91400.11878736554706325No Hit
CGCGACCTCAGATCAGACGTGG90940.11818952979048065No Hit
TAGCAGCACGTAAATATTGGCGT90380.1174617297389888No Hit
ACCACAGGGTAGAACCACGGAC89500.11631804394378732No Hit
AACATTCAACGCTGTCGGTGA89050.11573320461669566No Hit
ATCCCACCGCTGCCACCA88620.11517435814858584No Hit
CAAAGTGCTGTTCGTGCAGGT86630.11258806867989159No Hit
AACATTCAACGCTGTCGGTGAGA86040.11182127933992694No Hit
ACTGGACTTGGAGTCAGAAGG85120.11062560782676176No Hit
TTCACAGTGGCTAAGTTCCG84970.11043066138439787No Hit
CAAAGTGCTGTTCGTGCAGGTAG84220.1094559291725784No Hit
TGAGGTAGTAAGTTGTATTGTT81250.10559598961377341No Hit
TATTGCACTTGTCCCGGCCTGTAAT81020.1052970717354821No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)