FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6981842
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6419949.195195193474731No Hit
CCCCCCACTGCTAAATTTGACTG5837568.361060018258792No Hit
TTCAAGTAATCCAGGATAGGCT2397523.433936201936395No Hit
CCCCCACTGCTAAATTTGACTG2044912.928897560271344No Hit
AACATTCAACGCTGTCGGTGAGT1750282.5069029061385235No Hit
TAGCAGCACGTAAATATTGGCG1295341.855298358226955No Hit
AAGCTGCCAGTTGAAGAACTGT1157271.6575425224460822No Hit
TCGTACGACTCTTAGCGG1094421.5675232982929146No Hit
AACATTCAACGCTGTCGGTGAG978161.4010056371943107No Hit
CATAAAGTAGAAAGCACTACT703151.0071124496945076No Hit
CGCCTGTCTGAGCGTCGCT678300.9715201231995797No Hit
GCCTGTCTGAGCGTCGCT566950.8120349901931324No Hit
ATCCCGGACGAGCCCCCA447630.6411345315462595No Hit
TAGCTTATCAGACTGATGTTGAC419410.6007153986011141No Hit
ACTGGACTTGGAGTCAGAAGGC418960.6000708695498982No Hit
CCCATAAAGTAGAAAGCACTAC415070.5944992739738311No Hit
TAGCTTATCAGACTGATGTTGA402880.5770396981197798No Hit
CTCCCACTGCTTCACTTGACTAG402050.5758509000919815No Hit
TATTGCACTTGTCCCGGCCTGT400650.5738456985993095No Hit
CAACGGAATCCCAAAAGCAGCTGT376270.5389265468912072No Hit
TATTGCACTTGTCCCGGCCTGTAA374800.5368210853239016No Hit
TTCAAGTAATTCAGGATAGGTT369790.5296453285536968No Hit
AGCAGCATTGTACAGGGCTATGA369700.5295164227434537No Hit
CACCCGGCTGTGTGCACATGTGC368210.5273823154405385No Hit
CATTGCACTTGTCTCGGTCTGA366920.5255346654937193No Hit
TATTGCACTTGTCCCGGCCTGTAT346330.4960438806836362No Hit
TGAGGTAGTAGGTTGTATAGTT346270.49595794347680744No Hit
CCCATAAAGTAGAAAGCACT312240.4472172243370732No Hit
AACCGGGCGGAAACACCA299290.42866911052985734No Hit
CTACGCCTGTCTGAGCGTCGCT294950.42245298590257413No Hit
CAACACCAGTCGATGGGCTGT287900.4123553641001902No Hit
ACCGGGTGCTGTAGGCTTA269620.3861731617530159No Hit
TAGCTTATCAGACTGATGTTGACT266400.3815611983198703No Hit
CAACGGAATCCCAAAAGCAGCT257770.36920056340432794No Hit
TGAGGTAGTAGATTGTATAGTT245880.35217067358442083No Hit
ACCGGGTGCTGTAGGCTT217730.31185180071390906No Hit
TCCTGTCTGAGCGTCGCT216360.30988956782465144No Hit
CACTAGATTGTGAGCTCCTGGA213080.30519166718467705No Hit
TTCTCACTACTGCACTTGACTAG212900.30493385556419067No Hit
CTTCTCACTACTGCACTTGACTAG209070.2994481971949523No Hit
AGCTCGGTCTGAGGCCCCTCAGT207560.2972854441564275No Hit
TACGCCTGTCTGAGCGTCGCT205640.2945354535379059No Hit
CCCCCCACTGCTAAATTTGACTGGCT204200.2924729605740147No Hit
TATTGCACTTGTCCCGGCCTGTA202070.28942218973159234No Hit
CCCCCACTGCTAAATTTGACTGGCTT201550.2886774006057427No Hit
ACCGGGTGCTGTAGGCTTT188820.2704443898902324No Hit
ACGCCTGTCTGAGCGTCGCT184170.26378425636100045No Hit
CCCCCCACTGCTAAATTTGACTGG173510.2485160792810837No Hit
ATCCCACCGCTGCCACCA160130.2293520821582614No Hit
CACTAGATTGTGAGCTCCTGGAA157710.2258859481494998No Hit
TGTAAACATCCTTGACTGGAAGCT146080.20922845289251749No Hit
TGTAAACATCCTACACTCTCAGCT142510.20411518908620388No Hit
AACGGAATCCCAAAAGCAGCTG138690.19864385358477032No Hit
TTCAAGTAATCCAGGATAGGCTT137420.19682484937356073No Hit
AACATTCAACGCTGTCGGTGAGTT126160.18069730022535602No Hit
CAAAGAATTCTCCTTTTGGGCT125210.1793366277839No Hit
TTCACAGTGGCTAAGTTCCG117390.16813614516054645No Hit
ACCCCCCACTGCTAAATTTGACTG116460.16680411845470006No Hit
TTCACAGTGGCTAAGTTCCGC108300.15511665832598331No Hit
CAAAGAATTCTCCTTTTGGGCTT107410.1538419230913561No Hit
ACCACAGGGTAGAACCACGGAC103870.14877162788845694No Hit
CTCCCACTGCTTCACTTGACTAGCCT103700.14852813913577534No Hit
ACTGGACTTGGAGTCAGAAGGCT103410.14811277596943614No Hit
TGTAAACATCCCCGACTGGAAGCT102550.14688100933822335No Hit
TCGTTTCCCGGGCGGCGCACCA99990.14321435518019457No Hit
TTCAAGTAATCCAGGATAGGC99990.14321435518019457No Hit
ACTGGACTTGGAGTCAGAAGG96630.1384018715977818No Hit
CAAAGTGCTGTTCGTGCAGGT96540.1382729657875386No Hit
CCCATAAAGTAGAAAGCACTA92090.13189928961440262No Hit
CGCGACCTCAGATCAGACGT88060.12612717388906824No Hit
TCGTTTCCCGGCCAATGCACCA87320.12506728167151304No Hit
CCCCCCACTGCTAAATTTGACTGT86530.12393577511493384No Hit
ACTGGACTTGGAGTCAGAAGGCA83970.12026912095690508No Hit
CGCGACCTCAGATCAGACGTGGCG82000.11744751599935949No Hit
CGCGACCTCAGATCAGACGTGGC81800.11716105864326348No Hit
CGCGACCTCAGATCAGACGTGG80250.11494101413351948No Hit
AGCAGCATTGTACAGGGCTATGT79760.11423919361108428No Hit
TAGCAGCACGTAAATATTGGC79090.1132795614681627No Hit
TCCCACTGCTTCACTTGACTAG77230.11061550805646991No Hit
ACTTGACTTGGAGTCAGAAGGC74740.10704911397307472No Hit
CAACGGAATCCCAAAAGCAGCTGA73480.10524443262966993No Hit
CAAAGTGCTGTTCGTGCAGGTAG73180.10481474659552593No Hit
TTCACAGTGGCTAAGTTCTGC70560.10106215523066836No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)