FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003947690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6682127
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG74932111.213809614812769No Hit
CAACGGAATCCCAAAAGCAGCTG4021636.0184878258075605No Hit
AACATTCAACGCTGTCGGTGAGT2191733.279988542570352No Hit
AAGCTGCCAGTTGAAGAACTGT2053703.0734225793673184No Hit
CCCCCACTGCTAAATTTGACTG1972132.9513506702282073No Hit
TAGCAGCACGTAAATATTGGCG1896852.8386919314763097No Hit
TTCAAGTAATCCAGGATAGGCT1822122.726856283934741No Hit
TAGCTTATCAGACTGATGTTGAC1415122.117768788291513No Hit
AACATTCAACGCTGTCGGTGAG1249891.8704972234140416No Hit
TATTGCACTTGTCCCGGCCTGTAA1102121.6493550631408234No Hit
TAGCTTATCAGACTGATGTTGACT1058061.5834179745461288No Hit
TATTGCACTTGTCCCGGCCTGTAT959591.4360547173078273No Hit
TGTAAACATCCTTGACTGGAAGCT931601.3941668573494639No Hit
TGAGGTAGTAGGTTGTATAGTT753531.1276798540345012No Hit
AGCAGCATTGTACAGGGCTATGA519750.7778211937606094No Hit
TAGCTTATCAGACTGATGTTGA514640.7701739281519192No Hit
TGAGGTAGTAGATTGTATAGTT479910.718199459543346No Hit
ACTGGACTTGGAGTCAGAAGGC470760.7045062148624233No Hit
TATTGCACTTGTCCCGGCCTGT458740.6865179305930582No Hit
CGCCTGTCTGAGCGTCGCT434350.6500175767386641No Hit
TGTAAACATCCCCGACTGGAAGCT433180.6482666372548741No Hit
TATTGCACTTGTCCCGGCCTGTA414900.6209100784824952No Hit
TTCAAGTAATTCAGGATAGGTT330430.49449823387074204No Hit
CATAAAGTAGAAAGCACTACT269430.403209935997924No Hit
CTTCTCACTACTGCACTTGACTAG254990.3816000504031127No Hit
CAACGGAATCCCAAAAGCAGCTGT250020.374162298920688No Hit
GGAATACCGGGTGCTGTAGGCTTT244410.36576676857533535No Hit
TGTAAACATCCTACACTCTCAGCT235960.3531210945257401No Hit
TGTCAGTTTGTCAAATACCCCAA235280.35210345448387914No Hit
TACGCCTGTCTGAGCGTCGCT234770.3513402244524835No Hit
CCCCCACTGCTAAATTTGACTGGCTT225300.3371680903400968No Hit
TTCTCACTACTGCACTTGACTAG222810.33344173195151783No Hit
AACATTCAACGCTGTCGGTGAGTT218490.3269767246267543No Hit
CCCATAAAGTAGAAAGCACTAC217930.3261386681216924No Hit
CTACGCCTGTCTGAGCGTCGCT206800.30948229508358643No Hit
CTCCCACTGCTTCACTTGACTAG190910.28570244175245396No Hit
TGAGGTAGTAGTTTGTACAGTT188880.28266448692160445No Hit
CACCCGGCTGTGTGCACATGTGC185380.27742663376496735No Hit
CCCCCCACTGCTAAATTTGACTGGCT185070.27696270962823666No Hit
CCCCCCACTGCTAAATTTGACTGG170020.25443994105469714No Hit
CATTGCACTTGTCTCGGTCTGA169080.2530332033497717No Hit
CTCCCACTGCTTCACTTGACTAGCCT165810.24813955197199936No Hit
GGAATACCGGGTGCTGTAGGCTT157530.23574828793286928No Hit
TTAATGCTAATCGTGATAGGGGTT157290.23538912085927127No Hit
TAGCTTATCAGACTGATGTTGACA156830.23470071730154188No Hit
CAAAGAATTCTCCTTTTGGGCTT151040.22603581165099076No Hit
CAAAGAATTCTCCTTTTGGGCT144760.2166376065585105No Hit
ACCCCCCACTGCTAAATTTGACTG144440.2161587171270465No Hit
TGTCAGTTTGTCAAATACCCCA136100.20367766131951698No Hit
ACTGGACTTGGAGTCAGAAGGCA134280.2009539776780657No Hit
GCGTTCGCGCTTTCCCCTG133780.2002057129414032No Hit
TTCAAGTAATCCAGGATAGGCTT132830.19878400994174458No Hit
CAACGGAATCCCAAAAGCAGCT132410.19815546756294816No Hit
CCAAGTCCTTCTGATCGAGGCCC124210.18588392588168406No Hit
TATTGCACTTGTCCCGGCCTGTAG122670.18357927049276374No Hit
AACCGGGCGGAAACACCA117510.17585717841040732No Hit
TAGCAGCACGTAAATATTGGC117360.17563269898940861No Hit
TGAGGTAGTAGTTTGTGCTGTT114670.17160703470616467No Hit
TGTAAACATCCCCGACTGGAAGC112630.1685541145805819No Hit
ACGCCTGTCTGAGCGTCGCT109400.16372032438174253No Hit
CACTAGATTGTGAGCTCCTGGAA108450.16229862138208387No Hit
AACGGAATCCCAAAAGCAGCTG104240.1559982322993861No Hit
CCCATAAAGTAGAAAGCACT102170.1529004162896036No Hit
TGAATACCGGGTGCTGTAGGCTTT101550.15197256801614217No Hit
AGCAGCATTGTACAGGGCTATGT98960.1480965566802307No Hit
CACTAGATTGTGAGCTCCTGGA98750.1477822854908325No Hit
TAGCAGCACGTAAATATTGGCGT96330.14416068416538627No Hit
TTATAAAGCAATGAGACTGATT95800.14336752354452406No Hit
TGTAAACATCCTACACTCAGCT94250.1410479028608705No Hit
GCCTGTCTGAGCGTCGCT94150.14089824991353803No Hit
ACTGGACTTGGAGTCAGAAGGCT92230.13802491332475422No Hit
TATTGCACTTGTCCCGGCCTGTT91480.13690251621976057No Hit
CGCGACCTCAGATCAGACGTGGCG90300.1351366114412372No Hit
CAAAGTGCTGTTCGTGCAGGTAG89040.13325098430484783No Hit
CAAGTCCTTCTGATCGAGGCCC88670.13269726839971765No Hit
TATTGCACTTGTCCCGGCCTGTAAT87730.13129053069479224No Hit
ATCACATTGCCAGGGATTTCCA85310.127668929369346No Hit
AGCTCGGTCTGAGGCCCCTCAGT85300.12765396407461277No Hit
ACTGGACTTGGAGTCAGAAGG83080.12433166864383152No Hit
CAACACCAGTCGATGGGCTGT82560.12355347331770257No Hit
GGGCTACGCCTGTCTGAGCGTCGCT79190.11851016899259771No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT78660.11771700837173553No Hit
TGAGGTAGTAGGTTGTGTGGTT76270.11414030293048906No Hit
TATTGCACTTGTCCCGGCCTGTAAA74240.11110234809963954No Hit
ACCACAGGGTAGAACCACGGAC72780.10891741506858521No Hit
ATGCACCTGGGCAAGGATTCTGA71950.10767529560572553No Hit
TGTAAACATCCTTGACTGGAAGC70190.10504140373267373No Hit
TTCAAGTAATCCAGGATAGGC69880.10457747959594303No Hit
TATTGCACTTGTCCCGGCCTGTTT69040.10332039483835012No Hit
ATAGGCGATAGAAATTGAAACCT68790.1029462624700189No Hit
CCTCGACACAAGGGTTTGT68490.10249730362802142No Hit
CTGCGTTCGCGCTTTCCCCTG68030.10180890007029199No Hit
AACATTCAACGCTGTCGGTGAGA67900.10161435123875975No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)