FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7629886
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG7113929.323756606586258No Hit
CAACGGAATCCCAAAAGCAGCTG4937856.471721857967472No Hit
AAGCTGCCAGTTGAAGAACTGT2275562.9824298816522288No Hit
TTCAAGTAATCCAGGATAGGCT2261392.9638581756005267No Hit
AACATTCAACGCTGTCGGTGAGT2252242.951865860119011No Hit
CCCCCACTGCTAAATTTGACTG2111092.766869649166449No Hit
TAGCAGCACGTAAATATTGGCG2052912.690616871602013No Hit
TAGCTTATCAGACTGATGTTGAC1257751.6484518903690044No Hit
AACATTCAACGCTGTCGGTGAG1212171.5887131210086232No Hit
GCCTGTCTGAGCGTCGCT1024431.3426543987682122No Hit
TAGCTTATCAGACTGATGTTGACT940751.2329804141241427No Hit
TATTGCACTTGTCCCGGCCTGTAA932771.2225215422615752No Hit
TGAGGTAGTAGGTTGTATAGTT858941.1257573179992466No Hit
TATTGCACTTGTCCCGGCCTGTAT826921.0837907669918003No Hit
TGTAAACATCCTTGACTGGAAGCT821911.0772244827773312No Hit
TAGCTTATCAGACTGATGTTGA648810.8503534653073453No Hit
TGAGGTAGTAGATTGTATAGTT554140.7262755957297395No Hit
TATTGCACTTGTCCCGGCCTGT514080.6737715347254205No Hit
AGCAGCATTGTACAGGGCTATGA512880.6721987720393201No Hit
TTCAAGTAATTCAGGATAGGTT440320.5770990549531146No Hit
CATAAAGTAGAAAGCACTACT435400.5706507279401029No Hit
TATTGCACTTGTCCCGGCCTGTA419860.5502834511551025No Hit
TGTAAACATCCCCGACTGGAAGCT373550.4895879178273437No Hit
CGCCTGTCTGAGCGTCGCT371440.4868224767709505No Hit
TTCTCACTACTGCACTTGACTAG366460.48029551162363365No Hit
GGAATACCGGGTGCTGTAGGCTTT360360.4723006346359565No Hit
AACCGGGCGGAAACACCA344990.45215616589815366No Hit
CCCCCACTGCTAAATTTGACTGGCTT338940.444226820689064No Hit
ACTGGACTTGGAGTCAGAAGGC335840.4401638504166379No Hit
CTTCTCACTACTGCACTTGACTAG301550.3952221566613184No Hit
CCCATAAAGTAGAAAGCACTAC299750.3928630126321678No Hit
TTAATGCTAATCGTGATAGGGGTT298240.3908839529188248No Hit
CAACGGAATCCCAAAAGCAGCTGT297300.38965195548137943No Hit
TGTCAGTTTGTCAAATACCCCAA266230.348930508267096No Hit
CTCCCACTGCTTCACTTGACTAG249370.3268331925273851No Hit
GGAATACCGGGTGCTGTAGGCTT248050.3251031535726746No Hit
CCCCCCACTGCTAAATTTGACTGGCT241060.31594181092613965No Hit
TGTAAACATCCTACACTCTCAGCT232880.3052208119492218No Hit
TGAGGTAGTAGTTTGTACAGTT223520.2929532629976385No Hit
CTCCCACTGCTTCACTTGACTAGCCT213450.2797551627901124No Hit
CCCCCCACTGCTAAATTTGACTGG213090.27928333398428234No Hit
AACATTCAACGCTGTCGGTGAGTT206650.27084284090221006No Hit
CATTGCACTTGTCTCGGTCTGA203220.26634736089110633No Hit
CTACGCCTGTCTGAGCGTCGCT191950.2515764979974799No Hit
ACCCCCCACTGCTAAATTTGACTG182430.23909924735441654No Hit
CACCCGGCTGTGTGCACATGTGC180040.23596682833793323No Hit
CAAAGAATTCTCCTTTTGGGCT172900.22660889035563572No Hit
TGTCAGTTTGTCAAATACCCCA171440.22469536242088023No Hit
TACGCCTGTCTGAGCGTCGCT157580.20652995339642033No Hit
TAGCTTATCAGACTGATGTTGACA154010.20185098440527158No Hit
TGATGTAGTAGGTTGTATAGTT152190.19946562766468595No Hit
TAGCAGCACGTAAATATTGGC138030.18090702796870098No Hit
TGAGGTAGTAGTTTGTGCTGTT137860.1806842199215034No Hit
CAAAGAATTCTCCTTTTGGGCTT130870.17152287727496845No Hit
CCCATAAAGTAGAAAGCACT129410.16960934934021296No Hit
TCGTACGACTCTTAGCGG123520.16188970582260337No Hit
TAGCAGCACGTAAATATTGGCGT120850.15839030884602995No Hit
TTCAAGTAATCCAGGATAGGCTT119050.15603116481687931No Hit
TTCACAGTGGCTAAGTTCCG118700.15557244236676668No Hit
ACGCCTGTCTGAGCGTCGCT109770.14386846671103604No Hit
TATTGCACTTGTCCCGGCCTGTAG109020.14288549003222328No Hit
ACCGGGTGCTGTAGGCTT106040.13897979602840724No Hit
TGTAAACATCCCCGACTGGAAGC105380.138114776551052No Hit
CAACGGAATCCCAAAAGCAGCT105240.13793128757100695No Hit
GGGCTACGCCTGTCTGAGCGTCGCT105080.1377215858795269No Hit
GCGTTCGCGCTTTCCCCTG103970.13626678039488402No Hit
TATTGCACTTGTCCCGGCCTGTT103260.13533622913894128No Hit
AACGGAATCCCAAAAGCAGCTG103210.13527069736035374No Hit
CAACACCAGTCGATGGGCTGT101390.1328853406197681No Hit
AGCTCGGTCTGAGGCCCCTCAGT100450.13165334318232277No Hit
TTATAAAGCAATGAGACTGATT100210.1313387906451027No Hit
AGCAGCATTGTACAGGGCTATGT99890.13091938726214258No Hit
ACTGGACTTGGAGTCAGAAGGCA98370.12892722119308206No Hit
TGATGTAGTAGATTGTATAGTT97860.12825879705148938No Hit
GAATACCGGGTGCTGTAGGCTTT96710.12675156614397645No Hit
TGTAAACATCCTACACTCAGCT96460.12642390725103886No Hit
CAAAGTGCTGTTCGTGCAGGTAG96000.1258210148880337No Hit
ACCGGGTGCTGTAGGCTTA95160.12472008100776343No Hit
ACCGGGTGCTGTAGGCTTT91680.1201590692180722No Hit
TGAGGTAGTAGGTTGTGTGGTT89380.11714460740304639No Hit
TTCAAGTAATCCAGGATAGGC89290.11702665020158887No Hit
GACCCGCGGGCGCTCTCCAGTCCT85690.11230836214328759No Hit
TGAGAACTGAATTCCATGGGTT84200.11035551514137958No Hit
ATCACATTGCCAGGGATTTCCA82550.10819296644799148No Hit
CACTAGATTGTGAGCTCCTGGAA82090.10759007408498633No Hit
CCCCCACTGCTAAATTTGACTGGCT81980.10744590417209379No Hit
TTCACAGTGGCTAAGTTCTGC81280.10652845927186855No Hit
CACTAGATTGTGAGCTCCTGGA79980.10482463302859309No Hit
TTCACAGTGGCTAAGTTCCGC79220.10382854999406282No Hit
GTCAGGATGGCCGAGCGGTCTAAGG78610.1030290622952951No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)