FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7907180
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG112097014.176609107165891No Hit
CAACGGAATCCCAAAAGCAGCTG81985110.368437293700156No Hit
CCCCCACTGCTAAATTTGACTG2459823.1108688559005864No Hit
AACATTCAACGCTGTCGGTGAGT2025572.5616844437587103No Hit
TTCAAGTAATCCAGGATAGGCT1984542.5097948952723983No Hit
AAGCTGCCAGTTGAAGAACTGT1705902.1574063066731757No Hit
TAGCAGCACGTAAATATTGGCG1654622.0925538561155808No Hit
AACATTCAACGCTGTCGGTGAG1154521.4600907023742977No Hit
TATTGCACTTGTCCCGGCCTGTAA1012031.2798873934828852No Hit
TATTGCACTTGTCCCGGCCTGTAT893271.1296947837282065No Hit
TAGCTTATCAGACTGATGTTGAC791641.001166028849729No Hit
TAGCTTATCAGACTGATGTTGACT680840.8610402191426021No Hit
CACCCGGCTGTGTGCACATGTGC592260.749015451779269No Hit
CAACGGAATCCCAAAAGCAGCTGT578800.7319929481812733No Hit
TAGCTTATCAGACTGATGTTGA566930.7169812752460423No Hit
CCCATAAAGTAGAAAGCACTAC539440.6822154042275501No Hit
TATTGCACTTGTCCCGGCCTGT495560.6267215366287349No Hit
AGCAGCATTGTACAGGGCTATGA488870.6182608717646494No Hit
CATAAAGTAGAAAGCACTACT485910.6145174385811376No Hit
ACTGGACTTGGAGTCAGAAGGC472060.5970017123677468No Hit
TGAGGTAGTAGGTTGTATAGTT443330.5606676463669729No Hit
TTCAAGTAATTCAGGATAGGTT367050.4641983614891782No Hit
TATTGCACTTGTCCCGGCCTGTA359790.4550168328025921No Hit
TGAGGTAGTAGATTGTATAGTT344690.43592026487319124No Hit
TGTAAACATCCTTGACTGGAAGCT317390.40139468179553267No Hit
AACCGGGCGGAAACACCA306600.3877488561029343No Hit
GGAATACCGGGTGCTGTAGGCTTT293870.3716495640670884No Hit
AGCTCGGTCTGAGGCCCCTCAGT278240.35188271925009923No Hit
CAAAGAATTCTCCTTTTGGGCT274630.34731724837426237No Hit
CATTGCACTTGTCTCGGTCTGA270790.34246090262267964No Hit
CAACGGAATCCCAAAAGCAGCT270070.34155033779425786No Hit
TGTAAACATCCCCGACTGGAAGCT269990.34144916392443325No Hit
CACTAGATTGTGAGCTCCTGGAA256590.32450254072880597No Hit
TCGTACGACTCTTAGCGG256220.32403461158086705No Hit
CACTAGATTGTGAGCTCCTGGA249730.3158268813913431No Hit
CCCCCACTGCTAAATTTGACTGGCTT239200.30250987077567476No Hit
CTCCCACTGCTTCACTTGACTAG238470.3015866592135249No Hit
CTTCTCACTACTGCACTTGACTAG235400.2977041119590043No Hit
CCCCCCACTGCTAAATTTGACTGGCT222540.2814404123846934No Hit
CCCATAAAGTAGAAAGCACT222440.2813139450474126No Hit
TTCTCACTACTGCACTTGACTAG222260.2810863038403072No Hit
CAAAGAATTCTCCTTTTGGGCTT215660.27273945957977436No Hit
ACCCCCCACTGCTAAATTTGACTG201350.25464198361489176No Hit
TGTAAACATCCTACACTCTCAGCT192050.24288052124777731No Hit
CCCCCCACTGCTAAATTTGACTGG190450.24085704385128454No Hit
CAACACCAGTCGATGGGCTGT178130.22527626789828992No Hit
CGCCTGTCTGAGCGTCGCT173920.2199519929987682No Hit
GGAATACCGGGTGCTGTAGGCTT167900.21233865929446402No Hit
AACATTCAACGCTGTCGGTGAGTT163250.2064579281109068No Hit
TCGTTTCCCGGGCGGCGCACCA158150.20000809390958596No Hit
ATCCCGGACGAGCCCCCA143870.18194855814588765No Hit
AACGGAATCCCAAAAGCAGCTG142960.18079770537663237No Hit
ACTGGACTTGGAGTCAGAAGGCA137740.17419611037057461No Hit
TTCAAGTAATCCAGGATAGGCTT130120.16455929926977758No Hit
CCCCCCACTGCTAAATTTGACTGT129490.16376255504490855No Hit
GCCTGTCTGAGCGTCGCT127870.16171378418095958No Hit
TTCACAGTGGCTAAGTTCCGC122120.15444191228731355No Hit
CTACGCCTGTCTGAGCGTCGCT118260.14956027306827466No Hit
GACCCGCGGGCGCTCTCCAGTCCT117890.14909234392033568No Hit
TATTGCACTTGTCCCGGCCTGTAG117840.1490291102516953No Hit
TCGTTTCCCGGCCAATGCACCA112840.14270574338765527No Hit
CGCGACCTCAGATCAGACGTGGCG111050.14044197805032896No Hit
TGAATACCGGGTGCTGTAGGCTTT106260.1343841925945786No Hit
ACTGGACTTGGAGTCAGAAGGCT104020.13155132423948868No Hit
CAAAGTGCTGTTCGTGCAGGTAG103740.13119721569510243No Hit
ACCGGGTGCTGTAGGCTTA102780.12998312925720676No Hit
CAACGGAATCCCAAAAGCAGCTGA101340.1281619996003632No Hit
TATTGCACTTGTCCCGGCCTGTAAT98780.12492443576597471No Hit
TGAGGTAGTAGTTTGTACAGTT97880.12378622973044752No Hit
CCCCCCACTGCTAAATTTGACTGTA97570.12339418098487705No Hit
CCCATAAAGTAGAAAGCACTA97370.12314124631031545No Hit
TGTCAGTTTGTCAAATACCCCAA96580.12214215434579712No Hit
TATTGCACTTGTCCCGGCCTGTT95790.12114306238127878No Hit
TGTAAACATCCCCGACTGGAAGC95260.12047278549369055No Hit
TAGCAGCACGTAAATATTGGC94300.11925869905579486No Hit
TACGCCTGTCTGAGCGTCGCT93460.11819637342263614No Hit
TATTGCACTTGTCCCGGCCTGTTT93440.11817107995517999No Hit
TTCACAGTGGCTAAGTTCCG92770.11732374879539861No Hit
TTCACAGTGGCTAAGTTCTGC92030.1163878904995207No Hit
AGCAGCATTGTACAGGGCTATGT90850.11489557591960725No Hit
ACCACAGGGTAGAACCACGGAC89990.11380795681899236No Hit
ACTGGACTTGGAGTCAGAAGG87580.11076009399052507No Hit
CCCCCCACTGCTAAATTTGACTGA86660.10959659448754171No Hit
ACTTGACTTGGAGTCAGAAGGC86570.109482773883989No Hit
TAGCTTATCAGACTGATGTTGACA84100.1063590306531532No Hit
ATCACATTGCCAGGGATTTCCA83370.10543581909100337No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)