FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8268015
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG6547847.919482487634577No Hit
CAACGGAATCCCAAAAGCAGCTG6046637.3132789430111105No Hit
AAGCTGCCAGTTGAAGAACTGT3163103.825706653894556No Hit
AACATTCAACGCTGTCGGTGAGT2586043.127764040099105No Hit
TTCAAGTAATCCAGGATAGGCT2489413.0108919734664243No Hit
TAGCAGCACGTAAATATTGGCG2334822.823918437496787No Hit
CCCCCACTGCTAAATTTGACTG1977662.3919405080905154No Hit
TAGCTTATCAGACTGATGTTGAC1490081.802222177874617No Hit
GCCTGTCTGAGCGTCGCT1330481.6091891463670542No Hit
AACATTCAACGCTGTCGGTGAG1297531.5693367755138325No Hit
TAGCTTATCAGACTGATGTTGACT1217061.4720099080613664No Hit
TGAGGTAGTAGGTTGTATAGTT1077451.303154384698141No Hit
TATTGCACTTGTCCCGGCCTGTAA1071301.2957160817923044No Hit
TATTGCACTTGTCCCGGCCTGTAT945661.1437569960867269No Hit
TAGCTTATCAGACTGATGTTGA941081.1382175770121365No Hit
TATTGCACTTGTCCCGGCCTGT784410.9487283223361351No Hit
CATAAAGTAGAAAGCACTACT765790.9262078019935862No Hit
TGTAAACATCCTTGACTGGAAGCT754190.9121778322850164No Hit
TGAGGTAGTAGATTGTATAGTT682360.8253008733051403No Hit
AGCAGCATTGTACAGGGCTATGA599320.7248656418741378No Hit
TTCAAGTAATTCAGGATAGGTT561290.6788691118726805No Hit
TCCTGTCTGAGCGTCGCT558000.6748899221880946No Hit
TTAATGCTAATCGTGATAGGGGTT548230.6630733011490666No Hit
CGCCTGTCTGAGCGTCGCT503130.6085257465062655No Hit
CCCATAAAGTAGAAAGCACTAC497330.6015107616519805No Hit
ACTGGACTTGGAGTCAGAAGGC463170.5601949198205374No Hit
CAACGGAATCCCAAAAGCAGCTGT441690.5342152862567375No Hit
TATTGCACTTGTCCCGGCCTGTA441130.5335379773742549No Hit
AACCGGGCGGAAACACCA415320.5023212947726873No Hit
TGTAAACATCCCCGACTGGAAGCT398260.4816875634599115No Hit
TCGTACGACTCTTAGCGG366320.44305676755545315No Hit
CACCCGGCTGTGTGCACATGTGC333510.40337372392285203No Hit
TGTAAACATCCTACACTCTCAGCT299260.3619490288781527No Hit
TGAGGTAGTAGTTTGTACAGTT279510.3380617959691655No Hit
CCCATAAAGTAGAAAGCACT272970.3301517958058857No Hit
CATTGCACTTGTCTCGGTCTGA255600.30914312564744983No Hit
ACCGGGTGCTGTAGGCTT254210.30746194824271605No Hit
ACCGGGTGCTGTAGGCTTT214310.2592036903658254No Hit
TACGCCTGTCTGAGCGTCGCT211470.2557687667475204No Hit
CTACGCCTGTCTGAGCGTCGCT192480.23280073875047397No Hit
ACCGGGTGCTGTAGGCTTA191210.23126469896341506No Hit
AACATTCAACGCTGTCGGTGAGTT191150.2311921301545776No Hit
AGCTCGGTCTGAGGCCCCTCAGT185150.22393524927083464No Hit
CAACACCAGTCGATGGGCTGT179100.21661789437972717No Hit
TGAGGTAGTAGTTTGTGCTGTT173190.20946986670924037No Hit
TAGCTTATCAGACTGATGTTGACA169840.2054181082158172No Hit
AACGGAATCCCAAAAGCAGCTG165900.20065275643549266No Hit
TTCTCACTACTGCACTTGACTAG164040.19840312336153235No Hit
CAACGGAATCCCAAAAGCAGCT155800.1884370069478587No Hit
TTCAAGTAATCCAGGATAGGCTT148760.17992226671093364No Hit
TGAGGTAGTAGGTTGTGTGGTT148460.1795594226667465No Hit
CAAAGAATTCTCCTTTTGGGCT145770.17630592107053508No Hit
CACTAGATTGTGAGCTCCTGGA143340.17336688431261918No Hit
TGAGAACTGAATTCCATGGGTT142830.17275004943750102No Hit
ACGCCTGTCTGAGCGTCGCT133550.16152607367064525No Hit
TTATAAAGCAATGAGACTGATT131510.15905873417017263No Hit
CCCCCACTGCTAAATTTGACTGGCTT130090.15734127236102013No Hit
TATTGCACTTGTCCCGGCCTGTT125020.15120920801425736No Hit
CAAAGTGCTGTTCGTGCAGGTAG119450.14447240359384933No Hit
TTCAAGTAATCCAGGATAGGC118580.14342015586570658No Hit
CACTAGATTGTGAGCTCCTGGAA116730.14118261759321918No Hit
CGCGACCTCAGATCAGACGTGG116480.14088024755639653No Hit
ACCACAGGGTAGAACCACGGAC115320.13947725058553959No Hit
TATTGCACTTGTCCCGGCCTGTAG114750.138787846901584No Hit
CAAAGAATTCTCCTTTTGGGCTT114700.13872737289421946No Hit
AGCAGCATTGTACAGGGCTATGT114140.1380500640117368No Hit
TAGCAGCACGTAAATATTGGC113070.13675592025413597No Hit
CTCCCACTGCTTCACTTGACTAG112760.1363809814084759No Hit
ACCCCCCACTGCTAAATTTGACTG105940.12813232680395475No Hit
TGAGGTAGTAGGTTGTATAGT104980.12697122586255588No Hit
CTTCTCACTACTGCACTTGACTAG103230.12485463560479752No Hit
ATCCCGGACGAGCCCCCA101430.12267757133967465No Hit
TGTAAACATCCTACACTCAGCT101380.1226170973323101No Hit
TGTAAACATCCCCGACTGGAAGC98890.11960549176555678No Hit
TCGTTTCCCGGCCAATGCACCA97840.11833553761090176No Hit
TTCACAGTGGCTAAGTTCCG97430.11783965075051267No Hit
CCCCCCACTGCTAAATTTGACTGG93150.11266307572010938No Hit
TTCACAGTGGCTAAGTTCTGC93100.11260260171274483No Hit
TGTCAGTTTGTCAAATACCCCA92850.11230023167592221No Hit
CAAAGTGCTGTTCGTGCAGGT92710.11213090445530154No Hit
TTCACAGTGGCTAAGTTCCGC92000.11127217355072529No Hit
TGTCAGTTTGTCAAATACCCCAA90220.10911929888854821No Hit
CCCCCCACTGCTAAATTTGACTGGCT89300.10800657715304098No Hit
AATGACACGATCACTCCCGTTG85260.10312027735798737No Hit
AGCAGCATTGTACAGGGCTATG84210.10185032320333236No Hit
CGCGACCTCAGATCAGACGTGGCG84120.10174146999007623No Hit
TCGTTTCCCGGGCGGCGCACCA83810.10136653114441617No Hit
AGAGGTAGTAGGTTGCATAGTT83740.10128186753410583No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)