FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947696

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947696
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5711424
Sequences flagged as poor quality0
Sequence length18-26
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG3112475.449551635459038No Hit
CCCCCCACTGCTAAATTTGACTG2753474.820986850214588No Hit
AAGCTGCCAGTTGAAGAACTGT2170103.799577828576551No Hit
TAGCAGCACGTAAATATTGGCG1647332.8842719433892494No Hit
TTCAAGTAATCCAGGATAGGCT1636112.8646271052543115No Hit
AACATTCAACGCTGTCGGTGAGT1525282.6705774251745273No Hit
GCCTGTCTGAGCGTCGCT1201432.1035559608251813No Hit
CGCCTGTCTGAGCGTCGCT901491.578397961699219No Hit
CCCCCACTGCTAAATTTGACTG868421.5204964646294865No Hit
AACATTCAACGCTGTCGGTGAG748961.311336717428088No Hit
AACCGGGCGGAAACACCA639911.1204035981219396No Hit
TAGCTTATCAGACTGATGTTGAC633961.1099858809291694No Hit
TGAGGTAGTAGGTTGTATAGTT599571.0497732264317972No Hit
TATTGCACTTGTCCCGGCCTGT591491.035626141571699No Hit
CATAAAGTAGAAAGCACTACT530440.92873511054336No Hit
TAGCTTATCAGACTGATGTTGA514510.9008436424961621No Hit
TCCTGTCTGAGCGTCGCT439300.7691601954258693No Hit
TATTGCACTTGTCCCGGCCTGTAA430100.7530521285059557No Hit
TAGCTTATCAGACTGATGTTGACT404760.7086849094026288No Hit
TATTGCACTTGTCCCGGCCTGTAT396860.6948529823735726No Hit
TTAATGCTAATCGTGATAGGGGTT392400.6870440716710928No Hit
ACCGGGTGCTGTAGGCTT388510.6802331607669121No Hit
TCGTACGACTCTTAGCGG387070.6777118981185778No Hit
TGTAAACATCCTTGACTGGAAGCT357220.625448224470815No Hit
AGCAGCATTGTACAGGGCTATGA319950.5601930446767741No Hit
TGAGGTAGTAGATTGTATAGTT315650.5526642742685537No Hit
TTCAAGTAATTCAGGATAGGTT306720.5370289440952029No Hit
ATCCCGGACGAGCCCCCA302010.5287823141829429No Hit
TATTGCACTTGTCCCGGCCTGTA290740.5090499322060488No Hit
ACCGGGTGCTGTAGGCTTT279310.48903740993489536No Hit
ACGCCTGTCTGAGCGTCGCT256510.4491174180029358No Hit
TACGCCTGTCTGAGCGTCGCT246090.4308732813392947No Hit
CTACGCCTGTCTGAGCGTCGCT243220.42584826481101734No Hit
CCCATAAAGTAGAAAGCACTAC221720.3882044127699152No Hit
ACCGGGTGCTGTAGGCTTA218580.38270665949507515No Hit
CAACGGAATCCCAAAAGCAGCTGT210700.3689097500028014No Hit
ACTGGACTTGGAGTCAGAAGGC210570.36868213601371563No Hit
TTCACAGTGGCTAAGTTCCG201360.35255656032541094No Hit
TGTAAACATCCCCGACTGGAAGCT198170.3469712632086149No Hit
CATTGCACTTGTCTCGGTCTGA177230.31030790219742044No Hit
CCCATAAAGTAGAAAGCACT154980.2713508925269775No Hit
CACCCGGCTGTGTGCACATGTGC153830.2693373841619883No Hit
CAACACCAGTCGATGGGCTGT149410.26159850853307337No Hit
TGTAAACATCCTACACTCTCAGCT146310.2561707903317981No Hit
TGAGGTAGTAGTTTGTACAGTT143430.2511282650351296No Hit
TTCTCACTACTGCACTTGACTAG141380.24753896751493146No Hit
AACATTCAACGCTGTCGGTGAGTT137220.240255319864188No Hit
GCGTTCGCGCTTTCCCCTG130140.2278591118432111No Hit
TGTCAGTTTGTCAAATACCCCAA121880.21339686915207134No Hit
TACCTGGTTGATCCTGCC115050.20143838034087475No Hit
TGAGGTAGTAGTTTGTGCTGTT109590.19187859279927388No Hit
ATCCCACCGCTGCCACCA98280.172076175748815No Hit
TGAGGTAGTAGGTTGTGTGGTT90310.1581216873410204No Hit
CGGCCCCGCCGGGGTCGGC89890.15738631906858955No Hit
AGCTCGGTCTGAGGCCCCTCAGT89160.15610817897603121No Hit
TATTGCACTTGTCCCGGCCTGTT85800.15022523279658453No Hit
TGATGTAGTAGGTTGTATAGTT85660.1499801100391076No Hit
TTCAAGTAATCCAGGATAGGC83470.14614568976143252No Hit
TGTAAACATCCTACACTCAGCT82830.14502512858439506No Hit
AACGGAATCCCAAAAGCAGCTG82230.14397460248092245No Hit
TCCCCGGCACCTCCACCA82170.14386954987057518No Hit
TAGCAGCACGTAAATATTGGC81820.14325674297688282No Hit
TGAGAACTGAATTCCATGGGTT81170.14211867303145415No Hit
TGTCAGTTTGTCAAATACCCCA80560.14105063815959032No Hit
CAACGGAATCCCAAAAGCAGCT79490.13917719994173083No Hit
TTATAAAGCAATGAGACTGATT79310.13886204211068903No Hit
CTCGACACAAGGGTTTGT79210.13868695442677692No Hit
AGCAGCATTGTACAGGGCTATG78550.13753137571295707No Hit
GGAATACCGGGTGCTGTAGGCTTT74670.13073797357716746No Hit
CCTCGACACAAGGGTTTGT74560.13054537712486414No Hit
TAGCAGCACGTAAATATTGGCGT73560.1287945002857431No Hit
CTTCTCACTACTGCACTTGACTAG73550.1287769915173519No Hit
TCGTTTCCCGGCCAATGCACCA73320.12837428984435406No Hit
CGCGACCTCAGATCAGACGTGG73100.12798909693974742No Hit
CAAAGAATTCTCCTTTTGGGCT71320.12487253616611199No Hit
ACCACAGGGTAGAACCACGGAC70780.12392706267298663No Hit
CACTAGATTGTGAGCTCCTGGA70180.12287653656951401No Hit
TGAGGTAGTAGGTTGTATAGT68880.12060039667865669No Hit
GGGCTACGCCTGTCTGAGCGTCGCT68010.11907713382862137No Hit
AGCTACATTGTCTGCTGGGTTT67950.11897208121827413No Hit
CAAAGTGCTGTTCGTGCAGGT67520.11821920417745208No Hit
TTCACAGTGGCTAAGTTCTG67370.11795657265158391No Hit
TTCACAGTGGCTAAGTTCTGC67060.1174138008314564No Hit
TTCACAGTGGCTAAGTTCCGC66070.11568043276072658No Hit
ATCACGTCGGGGTCACCA65730.11508513463542541No Hit
TTCAAGTAATCCAGGATAGGCTT65630.11491004695151333No Hit
GGAATACCGGGTGCTGTAGGCTT63030.11035776716979864No Hit
TTCACCACCTTCTCCACCCAGC62600.1096048901289766No Hit
ACTGGACTTGGAGTCAGAAGG61190.10713615378581594No Hit
CTCCCACTGCTTCACTTGACTAG60220.10543780325186854No Hit
TTAATGCTAATCGTGATAGGGGT59470.10412464562252777No Hit
AGCAGCATTGTACAGGGCTATGT58760.10288152306675183No Hit
TGTAAACATCCCCGACTGGAAGC58620.10263640030927489No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)